Characterization of novel lectins from Burkholderia
pseudomallei and Chromobacterium violaceum with seven-bladed
beta-propeller fold

J 2020

Characterization of novel lectins from Burkholderia pseudomallei and Chromobacterium violaceum with seven-bladed beta-propeller fold

SÝKOROVÁ, Petra, Jitka NOVOTNÁ, Gabriel DEMO, Guillaume POMPIDOR, Eva DUBSKÁ et. al.

Basic information

Original name

Characterization of novel lectins from Burkholderia pseudomallei and Chromobacterium violaceum with seven-bladed beta-propeller fold

Authors

SÝKOROVÁ, Petra (203 Czech Republic, belonging to the institution), Jitka NOVOTNÁ (203 Czech Republic, belonging to the institution), Gabriel DEMO (703 Slovakia, belonging to the institution), Guillaume POMPIDOR (276 Germany), Eva DUBSKÁ (203 Czech Republic, belonging to the institution), Jan KOMÁREK (203 Czech Republic, belonging to the institution), Eva FUJDIAROVÁ (203 Czech Republic, belonging to the institution), Josef HOUSER (203 Czech Republic, belonging to the institution), Lucia HÁRONÍKOVÁ (703 Slovakia, belonging to the institution), Annabelle VARROT (250 France), Nadya SHILOVA (643 Russian Federation), Anne IMBERTY (250 France), Nicolai BOVIN (643 Russian Federation), Martina POKORNÁ (203 Czech Republic, belonging to the institution) and Michaela WIMMEROVÁ (203 Czech Republic, guarantor, belonging to the institution)

Edition

International journal of biological macromolecules, Amsterdam, Elsevier Science BV, 2020, 0141-8130

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 6.953

RIV identification code

RIV/00216224:14740/20:00114036

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1016/j.ijbiomac.2019.10.200

UT WoS

000530068000110

Keywords in English

lectins; pathoges; Burkholderia pseudomallei; Chromobacterium violaceum

Abstract

V originále

Burkholderia pseudomallei and Chromobacterium violaceum are bacteria of tropical and subtropical soil and water that occasionally cause fatal infections in humans and animals. Microbial lectins mediate the adhesion of organisms to host cells, which is the first phase in the development of infection. Here we report the discovery of two novel lectins from the above-mentioned bacteria – BP39L and CV39L. The crystal structures revealed that the lectins possess a seven-bladed beta-propeller fold. Functional studies conducted on a series of oligo- and polysaccharides confirmed the preference of BP39L for mannosylated saccharides and CV39L for rather more complex polysaccharides with a monosaccharide preference for beta-L-fucose. The presented data indicate that the proteins belong to a currently unknown family of lectins.

Links

EF16_013/0001776, research and development project

Name: Česká infrastruktura pro integrativní strukturní biologii pro lidské zdraví

GA18-18964S, research and development project

Name: Lektiny a jejich úloha v interakci patogen/hostitel a buněčném rozpoznávání

Investor: Czech Science Foundation

LTC17076, research and development project

Name: Interdisciplinární přístup ke studiu biologických systémů na molekulární úrovni

Investor: Ministry of Education, Youth and Sports of the CR, Interdisciplinary approach to study biological systems at the molecular level, INTER-COST

MUNI/A/1252/2019, interní kód MU

Name: Podpora biochemického výzkumu v roce 2020

Investor: Masaryk University, Category A

MUNI/A/1575/2018, interní kód MU

Name: Podpora biochemického výzkumu v roce 2019

Investor: Masaryk University, Category A

90043, large research infrastructures

Name: CIISB

Citovat

SÝKOROVÁ, Petra, Jitka NOVOTNÁ, Gabriel DEMO, Guillaume POMPIDOR, Eva DUBSKÁ, Jan KOMÁREK, Eva FUJDIAROVÁ, Josef HOUSER, Lucia HÁRONÍKOVÁ, Annabelle VARROT, Nadya SHILOVA, Anne IMBERTY, Nicolai BOVIN, Martina POKORNÁ and Michaela WIMMEROVÁ. Characterization of novel lectins from Burkholderia pseudomallei and Chromobacterium violaceum with seven-bladed beta-propeller fold. International journal of biological macromolecules. Amsterdam: Elsevier Science BV, 2020, vol. 152, JUN, p. 1113-1124. ISSN 0141-8130. Available from: https://dx.doi.org/10.1016/j.ijbiomac.2019.10.200.

@article{1608903,
   author = {Sýkorová, Petra and Novotná, Jitka and Demo, Gabriel and Pompidor, Guillaume and Dubská, Eva and Komárek, Jan and Fujdiarová, Eva and Houser, Josef and Hároníková, Lucia and Varrot, Annabelle and Shilova, Nadya and Imberty, Anne and Bovin, Nicolai and Pokorná, Martina and Wimmerová, Michaela},
   article_location = {Amsterdam},
   article_number = {JUN},
   doi = {http://dx.doi.org/10.1016/j.ijbiomac.2019.10.200},
   keywords = {lectins; pathoges; Burkholderia pseudomallei; Chromobacterium violaceum},
   language = {eng},
   issn = {0141-8130},
   journal = {International journal of biological macromolecules},
   title = {Characterization of novel lectins from Burkholderia pseudomallei and Chromobacterium violaceum with seven-bladed beta-propeller fold},
   url = {http://dx.doi.org/10.1016/j.ijbiomac.2019.10.200},
   volume = {152},
   year = {2020}
}

TY  - JOUR
ID  - 1608903
AU  - Sýkorová, Petra - Novotná, Jitka - Demo, Gabriel - Pompidor, Guillaume - Dubská, Eva - Komárek, Jan - Fujdiarová, Eva - Houser, Josef - Hároníková, Lucia - Varrot, Annabelle - Shilova, Nadya - Imberty, Anne - Bovin, Nicolai - Pokorná, Martina - Wimmerová, Michaela
PY  - 2020
TI  - Characterization of novel lectins from Burkholderia pseudomallei and Chromobacterium violaceum with seven-bladed beta-propeller fold
JF  - International journal of biological macromolecules
VL  - 152
IS  - JUN
SP  - 1113-1124
EP  - 1113-1124
PB  - Elsevier Science BV
SN  - 01418130
KW  - lectins
KW  - pathoges
KW  - Burkholderia pseudomallei
KW  - Chromobacterium violaceum
UR  - http://dx.doi.org/10.1016/j.ijbiomac.2019.10.200
L2  - http://dx.doi.org/10.1016/j.ijbiomac.2019.10.200
N2  - Burkholderia pseudomallei and Chromobacterium violaceum are bacteria of tropical and subtropical soil and water that occasionally cause fatal infections in humans and animals. Microbial lectins mediate the adhesion of organisms to host cells, which is the first phase in the development of infection. Here we report the discovery of two novel lectins from the above-mentioned bacteria – BP39L and CV39L. The crystal structures revealed that the lectins possess a seven-bladed beta-propeller fold. Functional studies conducted on a series of oligo- and polysaccharides confirmed the preference of BP39L for mannosylated saccharides and CV39L for rather more complex polysaccharides with a monosaccharide preference for beta-L-fucose. The presented data indicate that the proteins belong to a currently unknown family of lectins.
ER  -

SÝKOROVÁ, Petra, Jitka NOVOTNÁ, Gabriel DEMO, Guillaume POMPIDOR, Eva DUBSKÁ, Jan KOMÁREK, Eva FUJDIAROVÁ, Josef HOUSER, Lucia HÁRONÍKOVÁ, Annabelle VARROT, Nadya SHILOVA, Anne IMBERTY, Nicolai BOVIN, Martina POKORNÁ and Michaela WIMMEROVÁ. Characterization of novel lectins from Burkholderia pseudomallei and Chromobacterium violaceum with seven-bladed beta-propeller fold. \textit{International journal of biological macromolecules}. Amsterdam: Elsevier Science BV, 2020, vol.~152, JUN, p.~1113-1124. ISSN~0141-8130. Available from: https://dx.doi.org/10.1016/j.ijbiomac.2019.10.200.

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