Detailed Information on Publication Record
2020
Calcium signaling affects migration and proliferation differently in individual cancer cells due to nifedipine treatment
CHOVANCOVÁ, Barbora, Veronika LISKOVÁ, Svetlana MIKLIKOVA, Sona HUDECOVA, Petr BABULA et. al.Basic information
Original name
Calcium signaling affects migration and proliferation differently in individual cancer cells due to nifedipine treatment
Authors
CHOVANCOVÁ, Barbora (703 Slovakia), Veronika LISKOVÁ (703 Slovakia), Svetlana MIKLIKOVA (703 Slovakia), Sona HUDECOVA (703 Slovakia), Petr BABULA (203 Czech Republic, belonging to the institution), Adela PENESOVA (703 Slovakia), Angelika SEVCIKOVA (703 Slovakia), Erika DURINIKOVA (703 Slovakia), Marie NOVÁKOVÁ (203 Czech Republic, belonging to the institution), Miroslava MATUSKOVA (703 Slovakia) and Oľga KRIŽANOVÁ (703 Slovakia, guarantor, belonging to the institution)
Edition
Biochemical Pharmacology, Oxford, Elsevier, 2020, 0006-2952
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
30105 Physiology
Country of publisher
United Kingdom of Great Britain and Northern Ireland
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 5.858
RIV identification code
RIV/00216224:14110/20:00115241
Organization unit
Faculty of Medicine
UT WoS
000519219000014
Keywords in English
Breast cancer; Sodium calcium exchanger 1; Inositol 1_4_5-trisphosphate receptor; Apoptosis Migration
Tags
International impact, Reviewed
Změněno: 14/7/2020 09:47, Mgr. Tereza Miškechová
Abstract
V originále
Several papers have reported that calcium channel blocking drugs were associated with increased breast cancer risk and worsened prognosis. One of the most common signs of breast tumors is the presence of small deposits of calcium, known as microcalcifications. Therefore, we studied the effect of dihydropyridine nifedipine on selected calcium transport systems in MDA-MB-231 cells, originating from triple negative breast tumor and JIMT1 cells that represent a model of HER2-positive breast cancer, which possesses amplification of HER2 receptor, but cells do not response to HER2 inhibition treatment with trastuzumab. Also, we compared the effect of nifedipine on colorectal DLD1 and ovarian A2780 cancer cells. Both, inositol 1,4,5-trisphosphate receptor type 1 (IP3R1) and type 1 sodium calcium exchanger (NCX1) were upregulated due to nifedipine in DLD1 and A2780 cells, but not in breast cancer MDA-MB-231 and JIMT1 cells. On contrary to MDA-MB-231 and JIMT1 cells, in DLD1 and A2780 cells nifedipine induced apoptosis in a concentration-dependent manner. After NCX1 silencing and subsequent treatment with nifedipine, proliferation was decreased in MDA-MB-231, increased in DLD1 cells, and not changed in JIMT1 cells. Silencing of IP3R1 revealed increase in proliferation in DLD1 and JIMT1 cells, but caused decrease in proliferation in MDA-MB-231 cell line after nifedipine treatment. Interestingly, after nifedipine treatment migration was not significantly affected in any of tested cell lines after NCX1 silencing. Due to IP3R1 silencing, significant decrease in migration occurred in MDA-MB-231 cells after nifedipine treatment, but not in other tested cells. These results support different function of the NCX1 and IP3R1 in the invasiveness of various cancer cells due to nifedipine treatment.
Links
MUNI/A/1255/2018, interní kód MU |
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