Analysis of bicarbonate, phosphate and other anions in saliva by capillary electrophoresis with capacitively coupled contactless conductivity detection in diagnostics of gastroesophageal reflux disease

DOSEDĚLOVÁ, Věra, Pavol ĎURČ, Jiří DOLINA, Štefan KONEČNÝ, František FORET and Petr KUBÁŇ. Analysis of bicarbonate, phosphate and other anions in saliva by capillary electrophoresis with capacitively coupled contactless conductivity detection in diagnostics of gastroesophageal reflux disease. Electrophoresis. HOBOKEN: WILEY-BLACKWELL, 2020, vol. 41, 1-2, p. 116-122. ISSN 0173-0835. Available from: https://dx.doi.org/10.1002/elps.201900319.

Basic information

• Original name: Analysis of bicarbonate, phosphate and other anions in saliva by capillary electrophoresis with capacitively coupled contactless conductivity detection in diagnostics of gastroesophageal reflux disease
• Authors: DOSEDĚLOVÁ, Věra (203 Czech Republic, belonging to the institution), Pavol ĎURČ (703 Slovakia, belonging to the institution), Jiří DOLINA (203 Czech Republic, belonging to the institution), Štefan KONEČNÝ (703 Slovakia, belonging to the institution), František FORET (203 Czech Republic, belonging to the institution) and Petr KUBÁŇ (203 Czech Republic, guarantor, belonging to the institution).
• Edition: Electrophoresis, HOBOKEN, WILEY-BLACKWELL, 2020, 0173-0835.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 10406 Analytical chemistry
• Country of publisher: United States of America
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• Impact factor: Impact factor: 3.535
• RIV identification code: RIV/00216224:14740/20:00115254
• Organization unit: Central European Institute of Technology
• Doi: http://dx.doi.org/10.1002/elps.201900319
• UT WoS: 000495131500001
• Keywords in English: Bicarbonate; Capillary electrophoresis; Gastroesophageal reflux disease; Phosphate; Saliva
• Tags: 14110213, podil, rivok
• Tags: International impact, Reviewed

Abstract

Bicarbonate and phosphate constitute major salivary buffering components, and their importance consists in the neutralization of acidic gastric contents during reflux episodes. In this work, capillary electrophoresis with capacitively coupled contactless conductivity detector was applied for the analysis of bicarbonate, phosphate, and another inorganic (chloride, nitrite, nitrate, sulfate, thiocyanate) and organic anions (acetate, butyrate) to evaluate their levels in saliva. The background electrolytes of different composition and pH between 6.02-9.41 were assessed for the bicarbonate and phosphate determination by comparison of the real analyses of a model solution with the simulation by PeakMaster software. The optimized background electrolyte was composed of 10 mM 2-(N-morpholino)ethanesulfonic acid, 20 mM arginine, and 30 mu M cetyltrimethylammonium bromide, pH 8.95. Using this BGE, the anion levels were compared in saliva from 20 patients suffering from gastroesophageal reflux disease (GERD) and saliva from 12 healthy subjects. Bicarbonate levels were significantly elevated in saliva from GERD patients suggesting the possible applicability of bicarbonate as a biomarker in non-invasive diagnostics of GERD by CE-(CD)-D-4.

Links

• LQ1601, research and development project: Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

• NV17-31945A, research and development project: Name: Vývoj neinvazivní diagnostiky extraezofageálních projevů refluxní choroby jícnu