Analysis of bicarbonate, phosphate and other anions in saliva
by capillary electrophoresis with capacitively coupled
contactless conductivity detection in diagnostics of
gastroesophageal reflux disease

J 2020

Analysis of bicarbonate, phosphate and other anions in saliva by capillary electrophoresis with capacitively coupled contactless conductivity detection in diagnostics of gastroesophageal reflux disease

DOSEDĚLOVÁ, Věra, Pavol ĎURČ, Jiří DOLINA, Štefan KONEČNÝ, František FORET et. al.

Basic information

Original name

Analysis of bicarbonate, phosphate and other anions in saliva by capillary electrophoresis with capacitively coupled contactless conductivity detection in diagnostics of gastroesophageal reflux disease

Authors

DOSEDĚLOVÁ, Věra (203 Czech Republic, belonging to the institution), Pavol ĎURČ (703 Slovakia, belonging to the institution), Jiří DOLINA (203 Czech Republic, belonging to the institution), Štefan KONEČNÝ (703 Slovakia, belonging to the institution), František FORET (203 Czech Republic, belonging to the institution) and Petr KUBÁŇ (203 Czech Republic, guarantor, belonging to the institution)

Edition

Electrophoresis, HOBOKEN, WILEY-BLACKWELL, 2020, 0173-0835

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10406 Analytical chemistry

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 3.535

RIV identification code

RIV/00216224:14740/20:00115254

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1002/elps.201900319

UT WoS

000495131500001

Keywords in English

Bicarbonate; Capillary electrophoresis; Gastroesophageal reflux disease; Phosphate; Saliva

Abstract

V originále

Bicarbonate and phosphate constitute major salivary buffering components, and their importance consists in the neutralization of acidic gastric contents during reflux episodes. In this work, capillary electrophoresis with capacitively coupled contactless conductivity detector was applied for the analysis of bicarbonate, phosphate, and another inorganic (chloride, nitrite, nitrate, sulfate, thiocyanate) and organic anions (acetate, butyrate) to evaluate their levels in saliva. The background electrolytes of different composition and pH between 6.02-9.41 were assessed for the bicarbonate and phosphate determination by comparison of the real analyses of a model solution with the simulation by PeakMaster software. The optimized background electrolyte was composed of 10 mM 2-(N-morpholino)ethanesulfonic acid, 20 mM arginine, and 30 mu M cetyltrimethylammonium bromide, pH 8.95. Using this BGE, the anion levels were compared in saliva from 20 patients suffering from gastroesophageal reflux disease (GERD) and saliva from 12 healthy subjects. Bicarbonate levels were significantly elevated in saliva from GERD patients suggesting the possible applicability of bicarbonate as a biomarker in non-invasive diagnostics of GERD by CE-(CD)-D-4.

Links

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

NV17-31945A, research and development project

Name: Vývoj neinvazivní diagnostiky extraezofageálních projevů refluxní choroby jícnu

Citovat

DOSEDĚLOVÁ, Věra, Pavol ĎURČ, Jiří DOLINA, Štefan KONEČNÝ, František FORET and Petr KUBÁŇ. Analysis of bicarbonate, phosphate and other anions in saliva by capillary electrophoresis with capacitively coupled contactless conductivity detection in diagnostics of gastroesophageal reflux disease. Electrophoresis. HOBOKEN: WILEY-BLACKWELL, 2020, vol. 41, 1-2, p. 116-122. ISSN 0173-0835. Available from: https://dx.doi.org/10.1002/elps.201900319.

@article{1611436,
   author = {Dosedělová, Věra and Ďurč, Pavol and Dolina, Jiří and Konečný, Štefan and Foret, František and Kubáň, Petr},
   article_location = {HOBOKEN},
   article_number = {1-2},
   doi = {http://dx.doi.org/10.1002/elps.201900319},
   keywords = {Bicarbonate; Capillary electrophoresis; Gastroesophageal reflux disease; Phosphate; Saliva},
   language = {eng},
   issn = {0173-0835},
   journal = {Electrophoresis},
   title = {Analysis of bicarbonate, phosphate and other anions in saliva by capillary electrophoresis with capacitively coupled contactless conductivity detection in diagnostics of gastroesophageal reflux disease},
   url = {https://onlinelibrary.wiley.com/doi/full/10.1002/elps.201900319},
   volume = {41},
   year = {2020}
}

TY  - JOUR
ID  - 1611436
AU  - Dosedělová, Věra - Ďurč, Pavol - Dolina, Jiří - Konečný, Štefan - Foret, František - Kubáň, Petr
PY  - 2020
TI  - Analysis of bicarbonate, phosphate and other anions in saliva by capillary electrophoresis with capacitively coupled contactless conductivity detection in diagnostics of gastroesophageal reflux disease
JF  - Electrophoresis
VL  - 41
IS  - 1-2
SP  - 116-122
EP  - 116-122
PB  - WILEY-BLACKWELL
SN  - 01730835
KW  - Bicarbonate
KW  - Capillary electrophoresis
KW  - Gastroesophageal reflux disease
KW  - Phosphate
KW  - Saliva
UR  - https://onlinelibrary.wiley.com/doi/full/10.1002/elps.201900319
N2  - Bicarbonate and phosphate constitute major salivary buffering components, and their importance consists in the neutralization of acidic gastric contents during reflux episodes. In this work, capillary electrophoresis with capacitively coupled contactless conductivity detector was applied for the analysis of bicarbonate, phosphate, and another inorganic (chloride, nitrite, nitrate, sulfate, thiocyanate) and organic anions (acetate, butyrate) to evaluate their levels in saliva. The background electrolytes of different composition and pH between 6.02-9.41 were assessed for the bicarbonate and phosphate determination by comparison of the real analyses of a model solution with the simulation by PeakMaster software. The optimized background electrolyte was composed of 10 mM 2-(N-morpholino)ethanesulfonic acid, 20 mM arginine, and 30 mu M cetyltrimethylammonium bromide, pH 8.95. Using this BGE, the anion levels were compared in saliva from 20 patients suffering from gastroesophageal reflux disease (GERD) and saliva from 12 healthy subjects. Bicarbonate levels were significantly elevated in saliva from GERD patients suggesting the possible applicability of bicarbonate as a biomarker in non-invasive diagnostics of GERD by CE-(CD)-D-4.
ER  -

DOSEDĚLOVÁ, Věra, Pavol ĎURČ, Jiří DOLINA, Štefan KONEČNÝ, František FORET and Petr KUBÁŇ. Analysis of bicarbonate, phosphate and other anions in saliva by capillary electrophoresis with capacitively coupled contactless conductivity detection in diagnostics of gastroesophageal reflux disease. \textit{Electrophoresis}. HOBOKEN: WILEY-BLACKWELL, 2020, vol.~41, 1-2, p.~116-122. ISSN~0173-0835. Available from: https://dx.doi.org/10.1002/elps.201900319.

Detailed Information on Publication Record