Application of the Enzymatic Electrochemical Biosensors for
Monitoring Non-Competitive Inhibition of Enzyme Activity by
Heavy Metals

J 2019

Application of the Enzymatic Electrochemical Biosensors for Monitoring Non-Competitive Inhibition of Enzyme Activity by Heavy Metals

ASHRAFI, A.M., M. SYS, E. SEDLACKOVA, A.S. FARAG, V. ADAM et. al.

Basic information

Original name

Application of the Enzymatic Electrochemical Biosensors for Monitoring Non-Competitive Inhibition of Enzyme Activity by Heavy Metals

Authors

ASHRAFI, A.M. (203 Czech Republic), M. SYS (203 Czech Republic), E. SEDLACKOVA (203 Czech Republic), A.S. FARAG (203 Czech Republic), V. ADAM (203 Czech Republic), Jan PŘIBYL (203 Czech Republic, guarantor, belonging to the institution) and L. RICHTERA (203 Czech Republic)

Edition

Sensors, Basel, MDPI AG, 2019, 1424-8220

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10406 Analytical chemistry

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 3.275

RIV identification code

RIV/00216224:14740/19:00112781

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.3390/s19132939

UT WoS

000477045000100

Keywords in English

glucose oxidase; heavy metals; amperometric biosensor; non-competitive inhibition

Abstract

V originále

The inhibition effect of the selected heavy metals (Ag+, Cd2+, Cu2+, and Hg2+) on glucose oxidase (GOx) enzyme from Aspergillus niger (EC 1.1.3.4.) was studied using a new amperometric biosensor with an electrochemical transducer based on a glassy carbon electrode (GCE) covered with a thin layer of multi-wall carbon nanotubes (MWCNTs) incorporated with ruthenium(IV) oxide as a redox mediator. Direct adsorption of multi-wall carbon nanotubes (MWCNTs) and subsequent covering with Nafion((R)) layer was used for immobilization of Gox. The analytical figures of merit of the developed glucose (Glc) biosensor are sufficient for determination of Glc in body fluids in clinical analysis. From all tested heavy metals, mercury(II) has the highest inhibition effect. However, it is necessary to remember that cadmium and silver ions also significantly inhibit the catalytic activity of Gox. Therefore, the development of Gox biosensors for selective indirect determination of each heavy metal still represents a challenge in the field of bioelectroanalysis. It can be concluded that amperometric biosensors, differing in the utilized enzyme, could find their application in the toxicity studies of various poisons.

Links

LM2015043, research and development project

Name: Česká infrastruktura pro integrativní strukturní biologii (Acronym: CIISB)

Investor: Ministry of Education, Youth and Sports of the CR

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

ASHRAFI, A.M., M. SYS, E. SEDLACKOVA, A.S. FARAG, V. ADAM, Jan PŘIBYL and L. RICHTERA. Application of the Enzymatic Electrochemical Biosensors for Monitoring Non-Competitive Inhibition of Enzyme Activity by Heavy Metals. Sensors. Basel: MDPI AG, 2019, vol. 19, No 13, p. 2939-2952. ISSN 1424-8220. Available from: https://dx.doi.org/10.3390/s19132939.

@article{1612117,
   author = {Ashrafi, A.M. and Sys, M. and Sedlackova, E. and Farag, A.S. and Adam, V. and Přibyl, Jan and Richtera, L.},
   article_location = {Basel},
   article_number = {13},
   doi = {http://dx.doi.org/10.3390/s19132939},
   keywords = {glucose oxidase; heavy metals; amperometric biosensor; non-competitive inhibition},
   language = {eng},
   issn = {1424-8220},
   journal = {Sensors},
   title = {Application of the Enzymatic Electrochemical Biosensors for Monitoring Non-Competitive Inhibition of Enzyme Activity by Heavy Metals},
   url = {https://www.mdpi.com/1424-8220/19/13/2939},
   volume = {19},
   year = {2019}
}

TY  - JOUR
ID  - 1612117
AU  - Ashrafi, A.M. - Sys, M. - Sedlackova, E. - Farag, A.S. - Adam, V. - Přibyl, Jan - Richtera, L.
PY  - 2019
TI  - Application of the Enzymatic Electrochemical Biosensors for Monitoring Non-Competitive Inhibition of Enzyme Activity by Heavy Metals
JF  - Sensors
VL  - 19
IS  - 13
SP  - 2939
EP  - 2939
PB  - MDPI AG
SN  - 14248220
KW  - glucose oxidase
KW  - heavy metals
KW  - amperometric biosensor
KW  - non-competitive inhibition
UR  - https://www.mdpi.com/1424-8220/19/13/2939
L2  - https://www.mdpi.com/1424-8220/19/13/2939
N2  - The inhibition effect of the selected heavy metals (Ag+, Cd2+, Cu2+, and Hg2+) on glucose oxidase (GOx) enzyme from Aspergillus niger (EC 1.1.3.4.) was studied using a new amperometric biosensor with an electrochemical transducer based on a glassy carbon electrode (GCE) covered with a thin layer of multi-wall carbon nanotubes (MWCNTs) incorporated with ruthenium(IV) oxide as a redox mediator. Direct adsorption of multi-wall carbon nanotubes (MWCNTs) and subsequent covering with Nafion((R)) layer was used for immobilization of Gox. The analytical figures of merit of the developed glucose (Glc) biosensor are sufficient for determination of Glc in body fluids in clinical analysis. From all tested heavy metals, mercury(II) has the highest inhibition effect. However, it is necessary to remember that cadmium and silver ions also significantly inhibit the catalytic activity of Gox. Therefore, the development of Gox biosensors for selective indirect determination of each heavy metal still represents a challenge in the field of bioelectroanalysis. It can be concluded that amperometric biosensors, differing in the utilized enzyme, could find their application in the toxicity studies of various poisons.
ER  -

ASHRAFI, A.M., M. SYS, E. SEDLACKOVA, A.S. FARAG, V. ADAM, Jan PŘIBYL and L. RICHTERA. Application of the Enzymatic Electrochemical Biosensors for Monitoring Non-Competitive Inhibition of Enzyme Activity by Heavy Metals. \textit{Sensors}. Basel: MDPI AG, 2019, vol.~19, No~13, p.~2939-2952. ISSN~1424-8220. Available from: https://dx.doi.org/10.3390/s19132939.

Detailed Information on Publication Record