Biphasic calcium phosphate scaffolds with controlled pore size
distribution prepared by in-situ foaming

J 2019

Biphasic calcium phosphate scaffolds with controlled pore size distribution prepared by in-situ foaming

NOVOTNA, Lenka, Lukáš KUČERA, Aleš HAMPL, Daniel DRDLIK, Jaroslav CIHLAR et. al.

Basic information

Original name

Biphasic calcium phosphate scaffolds with controlled pore size distribution prepared by in-situ foaming

Authors

NOVOTNA, Lenka (203 Czech Republic, guarantor), Lukáš KUČERA (203 Czech Republic, belonging to the institution), Aleš HAMPL (203 Czech Republic, belonging to the institution), Daniel DRDLIK (203 Czech Republic), Jaroslav CIHLAR (203 Czech Republic) and Jaroslav CIHLAR (203 Czech Republic)

Edition

MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS, AMSTERDAM, ELSEVIER SCIENCE BV, 2019, 0928-4931

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

20902 Bioprocessing technologies biocatalysis, fermentation

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 5.880

RIV identification code

RIV/00216224:14110/19:00112788

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1016/j.msec.2018.03.022

UT WoS

000455858300039

Keywords in English

Calcium phosphate; Scaffold; Porosity; Bioactivity; Biocompatibility

Abstract

V originále

In this study, a reproducible method of fabricating hierarchically 3D porous scaffolds with high porosity and pore interconnectivity is reported. The method is based on in-situ foaming of a dispersion of diisocyanate, polyol, water and hydroxyapatite (HA) to form a hard foamed HA/polyurethane composite which after heat treatment provided a bi-phase calcium phosphate scaffold. This technique, combining the advantages of polymer sponge and direct foaming methods, provides a better control over the macrostructure of the scaffold. A modification of the multi-scaled porous macrostructure of scaffolds produced by changing the ratio of input reactants and by sintering temperature was studied. The pore morphology, size, and distribution were characterized using a scanning electron microscope and mercury porosimetry. The pores were open and interconnected with multi-scale (from several nanometres to millimetres) sizes convenient for using in tissue engineering applications. The bioactivity was confirmed by growing an apatite layer on the surfaces after immersion in simulated body fluid. The material was biocompatible, as shown by using normal human adipose tissue-derived stem cells (ASC). When seeded onto the scaffolds, the ASC adhered and remained healthy while maintaining their typical morphology.

Links

LD15144, research and development project

Name: Buněčné a nebuněčné základy pro regeneraci kostí a zubů (Acronym: TissueENG)

Investor: Ministry of Education, Youth and Sports of the CR

LM2015041, research and development project

Name: CEITEC Nano

Investor: Ministry of Education, Youth and Sports of the CR

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

NOVOTNA, Lenka, Lukáš KUČERA, Aleš HAMPL, Daniel DRDLIK, Jaroslav CIHLAR and Jaroslav CIHLAR. Biphasic calcium phosphate scaffolds with controlled pore size distribution prepared by in-situ foaming. MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS. AMSTERDAM: ELSEVIER SCIENCE BV, 2019, vol. 95, FEB 1 2019, p. 363-370. ISSN 0928-4931. Available from: https://dx.doi.org/10.1016/j.msec.2018.03.022.

@article{1612166,
   author = {Novotna, Lenka and Kučera, Lukáš and Hampl, Aleš and Drdlik, Daniel and Cihlar, Jaroslav and Cihlar, Jaroslav},
   article_location = {AMSTERDAM},
   article_number = {FEB 1 2019},
   doi = {http://dx.doi.org/10.1016/j.msec.2018.03.022},
   keywords = {Calcium phosphate; Scaffold; Porosity; Bioactivity; Biocompatibility},
   language = {eng},
   issn = {0928-4931},
   journal = {MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS},
   title = {Biphasic calcium phosphate scaffolds with controlled pore size distribution prepared by in-situ foaming},
   url = {http://dx.doi.org/10.1016/j.msec.2018.03.022},
   volume = {95},
   year = {2019}
}

TY  - JOUR
ID  - 1612166
AU  - Novotna, Lenka - Kučera, Lukáš - Hampl, Aleš - Drdlik, Daniel - Cihlar, Jaroslav - Cihlar, Jaroslav
PY  - 2019
TI  - Biphasic calcium phosphate scaffolds with controlled pore size distribution prepared by in-situ foaming
JF  - MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
VL  - 95
IS  - FEB 1 2019
SP  - 363-370
EP  - 363-370
PB  - ELSEVIER SCIENCE BV
SN  - 09284931
KW  - Calcium phosphate
KW  - Scaffold
KW  - Porosity
KW  - Bioactivity
KW  - Biocompatibility
UR  - http://dx.doi.org/10.1016/j.msec.2018.03.022
L2  - http://dx.doi.org/10.1016/j.msec.2018.03.022
N2  - In this study, a reproducible method of fabricating hierarchically 3D porous scaffolds with high porosity and pore interconnectivity is reported. The method is based on in-situ foaming of a dispersion of diisocyanate, polyol, water and hydroxyapatite (HA) to form a hard foamed HA/polyurethane composite which after heat treatment provided a bi-phase calcium phosphate scaffold. This technique, combining the advantages of polymer sponge and direct foaming methods, provides a better control over the macrostructure of the scaffold. A modification of the multi-scaled porous macrostructure of scaffolds produced by changing the ratio of input reactants and by sintering temperature was studied. The pore morphology, size, and distribution were characterized using a scanning electron microscope and mercury porosimetry. The pores were open and interconnected with multi-scale (from several nanometres to millimetres) sizes convenient for using in tissue engineering applications. The bioactivity was confirmed by growing an apatite layer on the surfaces after immersion in simulated body fluid. The material was biocompatible, as shown by using normal human adipose tissue-derived stem cells (ASC). When seeded onto the scaffolds, the ASC adhered and remained healthy while maintaining their typical morphology.
ER  -

NOVOTNA, Lenka, Lukáš KUČERA, Aleš HAMPL, Daniel DRDLIK, Jaroslav CIHLAR and Jaroslav CIHLAR. Biphasic calcium phosphate scaffolds with controlled pore size distribution prepared by in-situ foaming. \textit{MATERIALS SCIENCE \&{}amp; ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS}. AMSTERDAM: ELSEVIER SCIENCE BV, 2019, vol.~95, FEB 1 2019, p.~363-370. ISSN~0928-4931. Available from: https://dx.doi.org/10.1016/j.msec.2018.03.022.

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