The Influence of Quadruplex Structure in Proximity to P53
Target Sequences on the Transactivation Potential of P53 Alpha
Isoforms

J 2020

The Influence of Quadruplex Structure in Proximity to P53 Target Sequences on the Transactivation Potential of P53 Alpha Isoforms

PORUBIAKOVÁ, Otília, Natália BOHÁLOVÁ, Alberto INGA, Natália VADOVIČOVÁ, Jan COUFAL et. al.

Basic information

Original name

The Influence of Quadruplex Structure in Proximity to P53 Target Sequences on the Transactivation Potential of P53 Alpha Isoforms

Authors

PORUBIAKOVÁ, Otília (703 Slovakia), Natália BOHÁLOVÁ (703 Slovakia, belonging to the institution), Alberto INGA (380 Italy), Natália VADOVIČOVÁ (203 Czech Republic, belonging to the institution), Jan COUFAL (203 Czech Republic), Miroslav FOJTA (203 Czech Republic) and Václav BRÁZDA (203 Czech Republic, guarantor)

Edition

International Journal of Molecular Sciences, Basel, Molecular Diversity Preservation International, 2020, 1422-0067

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 5.923

RIV identification code

RIV/00216224:14310/20:00115260

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.3390/ijms21010127

UT WoS

000515378000127

Keywords in English

p53 protein; protein-DNA interaction; transactivation potential

Abstract

V originále

p53 is one of the most studied tumor suppressor proteins that plays an important role in basic biological processes including cell cycle, DNA damage response, apoptosis, and senescence. The human TP53 gene contains alternative promoters that produce N-terminally truncated proteins and can produce several isoforms due to alternative splicing. p53 function is realized by binding to a specific DNA response element (RE), resulting in the transactivation of target genes. Here, we evaluated the influence of quadruplex DNA structure on the transactivation potential of full-length and N-terminal truncated p53 alpha isoforms in a panel of S. cerevisiae luciferase reporter strains. Our results show that a G-quadruplex prone sequence is not sufficient for transcription activation by p53 alpha isoforms, but the presence of this feature in proximity to a p53 RE leads to a significant reduction of transcriptional activity and changes the dynamics between co-expressed p53 alpha isoforms.

Citovat

PORUBIAKOVÁ, Otília, Natália BOHÁLOVÁ, Alberto INGA, Natália VADOVIČOVÁ, Jan COUFAL, Miroslav FOJTA and Václav BRÁZDA. The Influence of Quadruplex Structure in Proximity to P53 Target Sequences on the Transactivation Potential of P53 Alpha Isoforms. International Journal of Molecular Sciences. Basel: Molecular Diversity Preservation International, 2020, vol. 21, No 1, p. 1-12. ISSN 1422-0067. Available from: https://dx.doi.org/10.3390/ijms21010127.

@article{1612996,
   author = {Porubiaková, Otília and Bohálová, Natália and Inga, Alberto and Vadovičová, Natália and Coufal, Jan and Fojta, Miroslav and Brázda, Václav},
   article_location = {Basel},
   article_number = {1},
   doi = {http://dx.doi.org/10.3390/ijms21010127},
   keywords = {p53 protein; protein-DNA interaction; transactivation potential},
   language = {eng},
   issn = {1422-0067},
   journal = {International Journal of Molecular Sciences},
   title = {The Influence of Quadruplex Structure in Proximity to P53 Target Sequences on the Transactivation Potential of P53 Alpha Isoforms},
   url = {https://www.mdpi.com/1422-0067/21/1/127},
   volume = {21},
   year = {2020}
}

TY  - JOUR
ID  - 1612996
AU  - Porubiaková, Otília - Bohálová, Natália - Inga, Alberto - Vadovičová, Natália - Coufal, Jan - Fojta, Miroslav - Brázda, Václav
PY  - 2020
TI  - The Influence of Quadruplex Structure in Proximity to P53 Target Sequences on the Transactivation Potential of P53 Alpha Isoforms
JF  - International Journal of Molecular Sciences
VL  - 21
IS  - 1
SP  - 1-12
EP  - 1-12
PB  - Molecular Diversity Preservation International
SN  - 14220067
KW  - p53 protein
KW  - protein-DNA interaction
KW  - transactivation potential
UR  - https://www.mdpi.com/1422-0067/21/1/127
L2  - https://www.mdpi.com/1422-0067/21/1/127
N2  - p53 is one of the most studied tumor suppressor proteins that plays an important role in basic biological processes including cell cycle, DNA damage response, apoptosis, and senescence. The human TP53 gene contains alternative promoters that produce N-terminally truncated proteins and can produce several isoforms due to alternative splicing. p53 function is realized by binding to a specific DNA response element (RE), resulting in the transactivation of target genes. Here, we evaluated the influence of quadruplex DNA structure on the transactivation potential of full-length and N-terminal truncated p53 alpha isoforms in a panel of S. cerevisiae luciferase reporter strains. Our results show that a G-quadruplex prone sequence is not sufficient for transcription activation by p53 alpha isoforms, but the presence of this feature in proximity to a p53 RE leads to a significant reduction of transcriptional activity and changes the dynamics between co-expressed p53 alpha isoforms.
ER  -

PORUBIAKOVÁ, Otília, Natália BOHÁLOVÁ, Alberto INGA, Natália VADOVIČOVÁ, Jan COUFAL, Miroslav FOJTA and Václav BRÁZDA. The Influence of Quadruplex Structure in Proximity to P53 Target Sequences on the Transactivation Potential of P53 Alpha Isoforms. \textit{International Journal of Molecular Sciences}. Basel: Molecular Diversity Preservation International, 2020, vol.~21, No~1, p.~1-12. ISSN~1422-0067. Available from: https://dx.doi.org/10.3390/ijms21010127.

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