PORUBIAKOVÁ, Otília, Natália BOHÁLOVÁ, Alberto INGA, Natália VADOVIČOVÁ, Jan COUFAL, Miroslav FOJTA and Václav BRÁZDA. The Influence of Quadruplex Structure in Proximity to P53 Target Sequences on the Transactivation Potential of P53 Alpha Isoforms. International Journal of Molecular Sciences. Basel: Molecular Diversity Preservation International, 2020, vol. 21, No 1, p. 1-12. ISSN 1422-0067. Available from: https://dx.doi.org/10.3390/ijms21010127.
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Basic information
Original name The Influence of Quadruplex Structure in Proximity to P53 Target Sequences on the Transactivation Potential of P53 Alpha Isoforms
Authors PORUBIAKOVÁ, Otília (703 Slovakia), Natália BOHÁLOVÁ (703 Slovakia, belonging to the institution), Alberto INGA (380 Italy), Natália VADOVIČOVÁ (203 Czech Republic, belonging to the institution), Jan COUFAL (203 Czech Republic), Miroslav FOJTA (203 Czech Republic) and Václav BRÁZDA (203 Czech Republic, guarantor).
Edition International Journal of Molecular Sciences, Basel, Molecular Diversity Preservation International, 2020, 1422-0067.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10608 Biochemistry and molecular biology
Country of publisher Switzerland
Confidentiality degree is not subject to a state or trade secret
WWW URL
Impact factor Impact factor: 5.923
RIV identification code RIV/00216224:14310/20:00115260
Organization unit Faculty of Science
Doi http://dx.doi.org/10.3390/ijms21010127
UT WoS 000515378000127
Keywords in English p53 protein; protein-DNA interaction; transactivation potential
Tags 14110513, podil, rivok
Tags International impact, Reviewed
Changed by Changed by: Mgr. Marie Šípková, DiS., učo 437722. Changed: 31/8/2021 16:32.
Abstract
p53 is one of the most studied tumor suppressor proteins that plays an important role in basic biological processes including cell cycle, DNA damage response, apoptosis, and senescence. The human TP53 gene contains alternative promoters that produce N-terminally truncated proteins and can produce several isoforms due to alternative splicing. p53 function is realized by binding to a specific DNA response element (RE), resulting in the transactivation of target genes. Here, we evaluated the influence of quadruplex DNA structure on the transactivation potential of full-length and N-terminal truncated p53 alpha isoforms in a panel of S. cerevisiae luciferase reporter strains. Our results show that a G-quadruplex prone sequence is not sufficient for transcription activation by p53 alpha isoforms, but the presence of this feature in proximity to a p53 RE leads to a significant reduction of transcriptional activity and changes the dynamics between co-expressed p53 alpha isoforms.
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