Bilateral activation of glial cells and cellular distribution
of the chemokine CCL2 and its receptor CCR2 in the trigeminal
subnucleus caudalis of trigeminal neuropathic pain model

J 2020

Bilateral activation of glial cells and cellular distribution of the chemokine CCL2 and its receptor CCR2 in the trigeminal subnucleus caudalis of trigeminal neuropathic pain model

KUBÍČKOVÁ, Lucie, Ilona KLUSÁKOVÁ and Petr DUBOVÝ

Basic information

Original name

Bilateral activation of glial cells and cellular distribution of the chemokine CCL2 and its receptor CCR2 in the trigeminal subnucleus caudalis of trigeminal neuropathic pain model

Authors

KUBÍČKOVÁ, Lucie (203 Czech Republic, belonging to the institution), Ilona KLUSÁKOVÁ (203 Czech Republic, belonging to the institution) and Petr DUBOVÝ (203 Czech Republic, guarantor, belonging to the institution)

Edition

Histochemistry and Cell Biology, New York, Springer, 2020, 0948-6143

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10601 Cell biology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 4.304

RIV identification code

RIV/00216224:14110/20:00115261

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1007/s00418-020-01850-4

UT WoS

000511060400001

Keywords in English

unilateral nerve injury; infraorbital nerve; microglia; astrocytes

Abstract

V originále

Glial cells activated by peripheral nerve injury contribute to the induction and maintenance of neuropathic pain by releasing neuromodulating cytokines and chemokines. We investigated the activation of microglia and astrocytes as well as the cellular distribution of the chemokine CCL2 and its receptor CCR2 in the trigeminal subnucleus caudalis (TSC) ipsilateral and contralateral to infraorbital nerve ligature (IONL). The left infraorbital nerve was ligated under aseptic conditions, and sham controls were operated without nerve ligature. Tactile hypersensitivity was significantly increased bilaterally in vibrissal pads of both sham- and IONL-operated animals from day 1 to 7 and tended to normalize in sham controls surviving for 14 days. Activated microglial cells significantly increased bilaterally in the TSC of both sham- and IONL-operated animals with a marked but gradual increase in the ipsilateral TSC from 1 to 7 days followed by a decrease by day 14. In contrast, robust activation of astrocytes was found bilaterally in the TSC of IONL-operated rats from 3 to 14 days with a transient activation in the ipsilateral TSC of sham-operated animals. Cellular distribution of CCL2 varied with survival time. CCL2 immunofluorescence was detected in neurons within 3 days and in astrocytes at later time points. In contrast, CCR2 was found only in astrocytes at all time points with CCR2 intensity being dominant in the ipsilateral TSC. In summary, our results reveal bilateral activation of microglial cells and astrocytes as well as changes in the cellular distribution of CCL2 and its receptor CCR2 in the TSC during the development and maintenance of orofacial neuropathic pain.

Links

MUNI/A/1086/2018, interní kód MU

Name: Reakce struktur nervové soustavy na poškození

Investor: Masaryk University, Category A

Citovat

KUBÍČKOVÁ, Lucie, Ilona KLUSÁKOVÁ and Petr DUBOVÝ. Bilateral activation of glial cells and cellular distribution of the chemokine CCL2 and its receptor CCR2 in the trigeminal subnucleus caudalis of trigeminal neuropathic pain model. Histochemistry and Cell Biology. New York: Springer, 2020, vol. 153, No 4, p. 239-255. ISSN 0948-6143. Available from: https://dx.doi.org/10.1007/s00418-020-01850-4.

@article{1613460,
   author = {Kubíčková, Lucie and Klusáková, Ilona and Dubový, Petr},
   article_location = {New York},
   article_number = {4},
   doi = {http://dx.doi.org/10.1007/s00418-020-01850-4},
   keywords = {unilateral nerve injury; infraorbital nerve; microglia; astrocytes},
   language = {eng},
   issn = {0948-6143},
   journal = {Histochemistry and Cell Biology},
   title = {Bilateral activation of glial cells and cellular distribution of the chemokine CCL2 and its receptor CCR2 in the trigeminal subnucleus caudalis of trigeminal neuropathic pain model},
   url = {https://link.springer.com/article/10.1007/s00418-020-01850-4},
   volume = {153},
   year = {2020}
}

TY  - JOUR
ID  - 1613460
AU  - Kubíčková, Lucie - Klusáková, Ilona - Dubový, Petr
PY  - 2020
TI  - Bilateral activation of glial cells and cellular distribution of the chemokine CCL2 and its receptor CCR2 in the trigeminal subnucleus caudalis of trigeminal neuropathic pain model
JF  - Histochemistry and Cell Biology
VL  - 153
IS  - 4
SP  - 239-255
EP  - 239-255
PB  - Springer
SN  - 09486143
KW  - unilateral nerve injury
KW  - infraorbital nerve
KW  - microglia
KW  - astrocytes
UR  - https://link.springer.com/article/10.1007/s00418-020-01850-4
L2  - https://link.springer.com/article/10.1007/s00418-020-01850-4
N2  - Glial cells activated by peripheral nerve injury contribute to the induction and maintenance of neuropathic pain by releasing neuromodulating cytokines and chemokines. We investigated the activation of microglia and astrocytes as well as the cellular distribution of the chemokine CCL2 and its receptor CCR2 in the trigeminal subnucleus caudalis (TSC) ipsilateral and contralateral to infraorbital nerve ligature (IONL). The left infraorbital nerve was ligated under aseptic conditions, and sham controls were operated without nerve ligature. Tactile hypersensitivity was significantly increased bilaterally in vibrissal pads of both sham- and IONL-operated animals from day 1 to 7 and tended to normalize in sham controls surviving for 14 days. Activated microglial cells significantly increased bilaterally in the TSC of both sham- and IONL-operated animals with a marked but gradual increase in the ipsilateral TSC from 1 to 7 days followed by a decrease by day 14. In contrast, robust activation of astrocytes was found bilaterally in the TSC of IONL-operated rats from 3 to 14 days with a transient activation in the ipsilateral TSC of sham-operated animals. Cellular distribution of CCL2 varied with survival time. CCL2 immunofluorescence was detected in neurons within 3 days and in astrocytes at later time points. In contrast, CCR2 was found only in astrocytes at all time points with CCR2 intensity being dominant in the ipsilateral TSC. In summary, our results reveal bilateral activation of microglial cells and astrocytes as well as changes in the cellular distribution of CCL2 and its receptor CCR2 in the TSC during the development and maintenance of orofacial neuropathic pain.
ER  -

KUBÍČKOVÁ, Lucie, Ilona KLUSÁKOVÁ and Petr DUBOVÝ. Bilateral activation of glial cells and cellular distribution of the chemokine CCL2 and its receptor CCR2 in the trigeminal subnucleus caudalis of trigeminal neuropathic pain model. \textit{Histochemistry and Cell Biology}. New York: Springer, 2020, vol.~153, No~4, p.~239-255. ISSN~0948-6143. Available from: https://dx.doi.org/10.1007/s00418-020-01850-4.

Detailed Information on Publication Record