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@article{1621177,
author = {Koutsoulas, Antonios and Čarnecká, Martina and Slanina, Jiří and Toth, Jaroslav and Slaninová, Iva},
article_location = {Basel},
article_number = {16},
doi = {http://dx.doi.org/10.3390/molecules24162921},
keywords = {12-O-methylcarnosic acid; cancer; carnosic acid; cytotoxicity; cell cycle; melanoma; microtubules; LC-MS; Salvia fruticosa; Salvia pomifera},
language = {eng},
issn = {1420-3049},
journal = {Molecules},
title = {Characterization of Phenolic Compounds and Antiproliferative Effects of Salvia pomifera and Salvia fruticosa Extracts},
url = {http://dx.doi.org/10.3390/molecules24162921},
volume = {24},
year = {2019}
}
TY - JOUR
ID - 1621177
AU - Koutsoulas, Antonios - Čarnecká, Martina - Slanina, Jiří - Toth, Jaroslav - Slaninová, Iva
PY - 2019
TI - Characterization of Phenolic Compounds and Antiproliferative Effects of Salvia pomifera and Salvia fruticosa Extracts
JF - Molecules
VL - 24
IS - 16
SP - 1-18
EP - 1-18
PB - MDPI
SN - 14203049
KW - 12-O-methylcarnosic acid
KW - cancer
KW - carnosic acid
KW - cytotoxicity
KW - cell cycle
KW - melanoma
KW - microtubules
KW - LC-MS
KW - Salvia fruticosa
KW - Salvia pomifera
UR - http://dx.doi.org/10.3390/molecules24162921
L2 - http://dx.doi.org/10.3390/molecules24162921
N2 - The phenolic compounds of methanolic extracts of Salvia pomifera and Salvia fruticosa were identified by liquid chromatography tandem mass spectrometry. Carnosic acid and its metabolite carnosol were the most abundant terpene phenolic compounds of S. fruticosa, while they were completely absent in S. pomifera. The main terpene phenolic constituent of S. pomifera was 12-O-methylcarnosic acid and its mass/mass fragmentation pathway was explained. The detailed mechanism of carnosic acid oxidation to carnosol was suggested. The effects of Salvia extracts and/or carnosic acid, the main diterpene phenolic component of S. fruticosa, on the proliferation and cell cycle of two melanoma cell lines (A375, Mel JuSo) and human fibroblast cell line (HFF) were investigated by MTT assay, PI-exclusion assay and flow cytometry cell cycle analysis. Extract of S. fruticosa more efficiently than S. pomifera extract reduced the proliferation of the human melanoma cells. Carnosic acid showed the most significant effect. The first evidence that carnosic acid affects microtubule dynamics and arrests the cell cycle in the G2/M phase was provided. Collectively, our results demonstrate that these two Salvia species are plants of medicinal interest with perspective for further investigation. Carnosic acid could be the compound responsible for the biological activities of S. fruticosa extracts.
ER -
KOUTSOULAS, Antonios, Martina ČARNECKÁ, Jiří SLANINA, Jaroslav TOTH and Iva SLANINOVÁ. Characterization of Phenolic Compounds and Antiproliferative Effects of Salvia pomifera and Salvia fruticosa Extracts. \textit{Molecules}. Basel: MDPI, 2019, vol.~24, No~16, p.~1-18. ISSN~1420-3049. Available from: https://dx.doi.org/10.3390/molecules24162921.
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