Follicle-stimulating hormone administration affects amino acid
metabolism in mammalian oocytes

J 2019

Follicle-stimulating hormone administration affects amino acid metabolism in mammalian oocytes

TETKOVA, Anna, Andrej SUSOR, Michal KUBELKA, Lucie NEMCOVA, Denisa JANSOVA et. al.

Basic information

Original name

Follicle-stimulating hormone administration affects amino acid metabolism in mammalian oocytes

Authors

TETKOVA, Anna (203 Czech Republic), Andrej SUSOR (203 Czech Republic, guarantor), Michal KUBELKA (203 Czech Republic), Lucie NEMCOVA (203 Czech Republic), Denisa JANSOVA (203 Czech Republic), Michal DVORAN (203 Czech Republic), Edgar DEL LLANO (203 Czech Republic), Zuzana HOLUBCOVÁ (203 Czech Republic, belonging to the institution) and Jaroslav KALOUS (203 Czech Republic)

Edition

Biology of reproduction, CARY, OXFORD UNIV PRESS INC, 2019, 0006-3363

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10604 Reproductive biology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 3.322

RIV identification code

RIV/00216224:14110/19:00112932

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1093/biolre/ioz117

UT WoS

000509503400008

Keywords in English

oocyte; oocyte maturation; FSH/FSH receptor; translation

Abstract

V originále

Culture media used in assisted reproduction are commonly supplemented with gonadotropin hormones to support the nuclear and cytoplasmic maturation of in vitro matured oocytes. However, the effect of gonadotropins on protein synthesis in oocytes is yet to be fully understood. As published data have previously documented a positive in vitro effect of follicle-stimulating hormone (FSH) on cytoplasmic maturation, we exposed mouse denuded oocytes to FSH in order to evaluate the changes in global protein synthesis. We found that dose-dependent administration of FSH resulted in a decrease of methionine incorporation into de novo synthesized proteins in denuded mouse oocytes and oocytes cultured in cumulus-oocyte complexes. Similarly, FSH influenced methionine incorporation in additional mammalian species including human. Furthermore, we showed the expression of FSH-receptor protein in oocytes. We found that major translational regulators were not affected by FSH treatment; however, the amino acid uptake became impaired. We propose that the effect of FSH treatment on amino acid uptake is influenced by FSH receptor with the effect on oocyte metabolism and physiology. Summary Sentence FSH treatment decrease methionine incorporation into de novo synthesized proteins in mouse, porcine, and bovine oocytes, and FSHR protein is expressed in oocytes and 2cell embryo.

Citovat

TETKOVA, Anna, Andrej SUSOR, Michal KUBELKA, Lucie NEMCOVA, Denisa JANSOVA, Michal DVORAN, Edgar DEL LLANO, Zuzana HOLUBCOVÁ and Jaroslav KALOUS. Follicle-stimulating hormone administration affects amino acid metabolism in mammalian oocytes. Biology of reproduction. CARY: OXFORD UNIV PRESS INC, 2019, vol. 101, No 4, p. 719-732. ISSN 0006-3363. Available from: https://dx.doi.org/10.1093/biolre/ioz117.

@article{1621517,
   author = {Tetkova, Anna and Susor, Andrej and Kubelka, Michal and Nemcova, Lucie and Jansova, Denisa and Dvoran, Michal and Del Llano, Edgar and Holubcová, Zuzana and Kalous, Jaroslav},
   article_location = {CARY},
   article_number = {4},
   doi = {http://dx.doi.org/10.1093/biolre/ioz117},
   keywords = {oocyte; oocyte maturation; FSH/FSH receptor; translation},
   language = {eng},
   issn = {0006-3363},
   journal = {Biology of reproduction},
   title = {Follicle-stimulating hormone administration affects amino acid metabolism in mammalian oocytes},
   url = {http://dx.doi.org/10.1093/biolre/ioz117},
   volume = {101},
   year = {2019}
}

TY  - JOUR
ID  - 1621517
AU  - Tetkova, Anna - Susor, Andrej - Kubelka, Michal - Nemcova, Lucie - Jansova, Denisa - Dvoran, Michal - Del Llano, Edgar - Holubcová, Zuzana - Kalous, Jaroslav
PY  - 2019
TI  - Follicle-stimulating hormone administration affects amino acid metabolism in mammalian oocytes
JF  - Biology of reproduction
VL  - 101
IS  - 4
SP  - 719-732
EP  - 719-732
PB  - OXFORD UNIV PRESS INC
SN  - 00063363
KW  - oocyte
KW  - oocyte maturation
KW  - FSH/FSH receptor
KW  - translation
UR  - http://dx.doi.org/10.1093/biolre/ioz117
L2  - http://dx.doi.org/10.1093/biolre/ioz117
N2  - Culture media used in assisted reproduction are commonly supplemented with gonadotropin hormones to support the nuclear and cytoplasmic maturation of in vitro matured oocytes. However, the effect of gonadotropins on protein synthesis in oocytes is yet to be fully understood. As published data have previously documented a positive in vitro effect of follicle-stimulating hormone (FSH) on cytoplasmic maturation, we exposed mouse denuded oocytes to FSH in order to evaluate the changes in global protein synthesis. We found that dose-dependent administration of FSH resulted in a decrease of methionine incorporation into de novo synthesized proteins in denuded mouse oocytes and oocytes cultured in cumulus-oocyte complexes. Similarly, FSH influenced methionine incorporation in additional mammalian species including human. Furthermore, we showed the expression of FSH-receptor protein in oocytes. We found that major translational regulators were not affected by FSH treatment; however, the amino acid uptake became impaired. We propose that the effect of FSH treatment on amino acid uptake is influenced by FSH receptor with the effect on oocyte metabolism and physiology. Summary Sentence FSH treatment decrease methionine incorporation into de novo synthesized proteins in mouse, porcine, and bovine oocytes, and FSHR protein is expressed in oocytes and 2cell embryo.
ER  -

TETKOVA, Anna, Andrej SUSOR, Michal KUBELKA, Lucie NEMCOVA, Denisa JANSOVA, Michal DVORAN, Edgar DEL LLANO, Zuzana HOLUBCOVÁ and Jaroslav KALOUS. Follicle-stimulating hormone administration affects amino acid metabolism in mammalian oocytes. \textit{Biology of reproduction}. CARY: OXFORD UNIV PRESS INC, 2019, vol.~101, No~4, p.~719-732. ISSN~0006-3363. Available from: https://dx.doi.org/10.1093/biolre/ioz117.

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