Informační systém MU
DONGEN VAN, Jacques J. M., Mirjam BURG VAN DER, Tomas KALINA, Martin PEREZ-ANDRES, Ester MEJSTRIKOVA, Marcela VLKOVÁ, Eduardo LOPEZ-GRANADOS, Marjolein WENTINK, Anne-Kathrin KIENZLER, Jan PHILIPPE, Ana E. SOUSA, Menno C. ZELM VAN, Elena BLANCO a Alberto ORFAO. EuroFlow-Based Flowcytometric Diagnostic Screening and Classification of Primary Immunodeficiencies of the Lymphoid System. Frontiers in Immunology. LAUSANNE: FRONTIERS MEDIA SA, 2019, roč. 10, JUN 13 2019, s. 1-21. ISSN 1664-3224. doi:10.3389/fimmu.2019.01271.
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Základní údaje
Originální název EuroFlow-Based Flowcytometric Diagnostic Screening and Classification of Primary Immunodeficiencies of the Lymphoid System
Autoři DONGEN VAN, Jacques J. M. (528 Nizozemsko, garant), Mirjam BURG VAN DER (528 Nizozemsko), Tomas KALINA (203 Česká republika), Martin PEREZ-ANDRES (724 Španělsko), Ester MEJSTRIKOVA (203 Česká republika), Marcela VLKOVÁ (203 Česká republika, domácí), Eduardo LOPEZ-GRANADOS (724 Španělsko), Marjolein WENTINK (528 Nizozemsko), Anne-Kathrin KIENZLER (826 Velká Británie), Jan PHILIPPE (56 Belgie), Ana E. SOUSA (620 Portugalsko), Menno C. ZELM VAN (528 Nizozemsko), Elena BLANCO (724 Španělsko) a Alberto ORFAO (724 Španělsko).
Vydání Frontiers in Immunology, LAUSANNE, FRONTIERS MEDIA SA, 2019, 1664-3224.
Další údaje
Originální jazyk angličtina
Typ výsledku Článek v odborném periodiku
Obor 30102 Immunology
Stát vydavatele Švýcarsko
Utajení není předmětem státního či obchodního tajemství
Impakt faktor Impact factor: 5.085
Kód RIV RIV/00216224:14110/19:00112952
Organizační jednotka Lékařská fakulta
UT WoS 000471898900001
Klíčová slova anglicky immunodeficiency; immunophenotyping; flow cytometry; diagnosis; classification; EuroFlow; standardization
Štítky 14110114, rivok
Příznaky Mezinárodní význam, Recenzováno
Změnil Změnila: Mgr. Tereza Miškechová, učo 341652. Změněno: 17. 2. 2020 07:57.
Guidelines for screening for primary immunodeficiencies (PID) are well-defined and several consensus diagnostic strategies have been proposed. These consensus proposals have only partially been implemented due to lack of standardization in laboratory procedures, particularly in flow cytometry. The main objectives of the EuroFlow Consortium were to innovate and thoroughly standardize the flowcytometric techniques and strategies for reliable and reproducible diagnosis and classification of PID of the lymphoid system. The proposed EuroFlow antibody panels comprise one orientation tube and seven classification tubes and corresponding databases of normal and PID samples. The 8-color 12-antibody PID Orientation tube (PIDOT) aims at identification and enumeration of the main lymphocyte and leukocyte subsets; this includes naive pre-germinal center (GC) and antigen-experienced post-GC memory B-cells and plasmablasts. The seven additional 8(-12)-color tubes can be used according to the EuroFlow PID algorithm in parallel or subsequently to the PIDOT for more detailed analysis of B-cell and T-cell subsets to further classify PID of the lymphoid system. The Pre-GC, Post-GC, and immunoglobulin heavy chain (IgH)-isotype B-cell tubes aim at identification and enumeration of B-cell subsets for evaluation of B-cell maturation blocks and specific defects in IgH-subclass production. The severe combined immunodeficiency (SCID) tube and T-cell memory/effector subset tube aim at identification and enumeration of T-cell subsets for assessment of T-cell defects, such as SCID. In case of suspicion of antibody deficiency, PIDOT is preferably directly combined with the IgH isotype tube(s) and in case of SCID suspicion (e.g., in newborn screening programs) the PIDOT is preferably directly combined with the SCID T-cell tube. The proposed >= 8-color antibody panels and corresponding reference databases combined with the EuroFlow PID algorithm are designed to provide fast, sensitive and cost-effective flowcytometric diagnosis of PID of the lymphoid system, easily applicable in multicenter diagnostic settings world-wide.
Zobrazeno: 20. 4. 2021 09:23