| DONGEN VAN, Jacques J. M., Mirjam BURG VAN DER, Tomas KALINA, Martin PEREZ-ANDRES, Ester MEJSTRIKOVA, Marcela VLKOVÁ, Eduardo LOPEZ-GRANADOS, Marjolein WENTINK, Anne-Kathrin KIENZLER, Jan PHILIPPE, Ana E. SOUSA, Menno C. ZELM VAN, Elena BLANCO a Alberto ORFAO. EuroFlow-Based Flowcytometric Diagnostic Screening and Classification of Primary Immunodeficiencies of the Lymphoid System. Online. Frontiers in Immunology. LAUSANNE: FRONTIERS MEDIA SA, 2019, roč. 10, JUN 13 2019, s. 1-21. ISSN 1664-3224. Dostupné z: https://dx.doi.org/10.3389/fimmu.2019.01271. [citováno 2024-04-23] |
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@article{1622836,
author = {Dongen van, Jacques J. M. and Burg van der, Mirjam and Kalina, Tomas and PerezandAndres, Martin and Mejstrikova, Ester and Vlková, Marcela and LopezandGranados, Eduardo and Wentink, Marjolein and Kienzler, AnneandKathrin and Philippe, Jan and Sousa, Ana E. and Zelm van, Menno C. and Blanco, Elena and Orfao, Alberto},
article_location = {LAUSANNE},
article_number = {JUN 13 2019},
doi = {http://dx.doi.org/10.3389/fimmu.2019.01271},
keywords = {immunodeficiency; immunophenotyping; flow cytometry; diagnosis; classification; EuroFlow; standardization},
language = {eng},
issn = {1664-3224},
journal = {Frontiers in Immunology},
title = {EuroFlow-Based Flowcytometric Diagnostic Screening and Classification of Primary Immunodeficiencies of the Lymphoid System},
url = {http://dx.doi.org/10.3389/fimmu.2019.01271},
volume = {10},
year = {2019}
}
TY - JOUR
ID - 1622836
AU - Dongen van, Jacques J. M. - Burg van der, Mirjam - Kalina, Tomas - Perez-Andres, Martin - Mejstrikova, Ester - Vlková, Marcela - Lopez-Granados, Eduardo - Wentink, Marjolein - Kienzler, Anne-Kathrin - Philippe, Jan - Sousa, Ana E. - Zelm van, Menno C. - Blanco, Elena - Orfao, Alberto
PY - 2019
TI - EuroFlow-Based Flowcytometric Diagnostic Screening and Classification of Primary Immunodeficiencies of the Lymphoid System
JF - Frontiers in Immunology
VL - 10
IS - JUN 13 2019
SP - 1-21
EP - 1-21
PB - FRONTIERS MEDIA SA
SN - 16643224
KW - immunodeficiency
KW - immunophenotyping
KW - flow cytometry
KW - diagnosis
KW - classification
KW - EuroFlow
KW - standardization
UR - http://dx.doi.org/10.3389/fimmu.2019.01271
L2 - http://dx.doi.org/10.3389/fimmu.2019.01271
N2 - Guidelines for screening for primary immunodeficiencies (PID) are well-defined and several consensus diagnostic strategies have been proposed. These consensus proposals have only partially been implemented due to lack of standardization in laboratory procedures, particularly in flow cytometry. The main objectives of the EuroFlow Consortium were to innovate and thoroughly standardize the flowcytometric techniques and strategies for reliable and reproducible diagnosis and classification of PID of the lymphoid system. The proposed EuroFlow antibody panels comprise one orientation tube and seven classification tubes and corresponding databases of normal and PID samples. The 8-color 12-antibody PID Orientation tube (PIDOT) aims at identification and enumeration of the main lymphocyte and leukocyte subsets; this includes naive pre-germinal center (GC) and antigen-experienced post-GC memory B-cells and plasmablasts. The seven additional 8(-12)-color tubes can be used according to the EuroFlow PID algorithm in parallel or subsequently to the PIDOT for more detailed analysis of B-cell and T-cell subsets to further classify PID of the lymphoid system. The Pre-GC, Post-GC, and immunoglobulin heavy chain (IgH)-isotype B-cell tubes aim at identification and enumeration of B-cell subsets for evaluation of B-cell maturation blocks and specific defects in IgH-subclass production. The severe combined immunodeficiency (SCID) tube and T-cell memory/effector subset tube aim at identification and enumeration of T-cell subsets for assessment of T-cell defects, such as SCID. In case of suspicion of antibody deficiency, PIDOT is preferably directly combined with the IgH isotype tube(s) and in case of SCID suspicion (e.g., in newborn screening programs) the PIDOT is preferably directly combined with the SCID T-cell tube. The proposed >= 8-color antibody panels and corresponding reference databases combined with the EuroFlow PID algorithm are designed to provide fast, sensitive and cost-effective flowcytometric diagnosis of PID of the lymphoid system, easily applicable in multicenter diagnostic settings world-wide.
ER -
DONGEN VAN, Jacques J. M., Mirjam BURG VAN DER, Tomas KALINA, Martin PEREZ-ANDRES, Ester MEJSTRIKOVA, Marcela VLKOVÁ, Eduardo LOPEZ-GRANADOS, Marjolein WENTINK, Anne-Kathrin KIENZLER, Jan PHILIPPE, Ana E. SOUSA, Menno C. ZELM VAN, Elena BLANCO a Alberto ORFAO. EuroFlow-Based Flowcytometric Diagnostic Screening and Classification of Primary Immunodeficiencies of the Lymphoid System. Online. \textit{Frontiers in Immunology}. LAUSANNE: FRONTIERS MEDIA SA, 2019, roč.~10, JUN 13 2019, s.~1-21. ISSN~1664-3224. Dostupné z: https://dx.doi.org/10.3389/fimmu.2019.01271. [citováno 2024-04-23]
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