Utilization of Red Nonionogenic Tenside Labeling, Isoelectric
Focusing, and Matrix-Assisted Laser Desorption/Ionization
Time-of-Flight Mass Spectrometry in the Identification of
Uropathogens in the Presence of a High Level of Albumin

J 2019

Utilization of Red Nonionogenic Tenside Labeling, Isoelectric Focusing, and Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry in the Identification of Uropathogens in the Presence of a High Level of Albumin

HORKA, Marie, Jiri SALPLACHTA, Filip RŮŽIČKA and Karel SLAIS

Basic information

Original name

Utilization of Red Nonionogenic Tenside Labeling, Isoelectric Focusing, and Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry in the Identification of Uropathogens in the Presence of a High Level of Albumin

Authors

HORKA, Marie (203 Czech Republic, guarantor), Jiri SALPLACHTA (203 Czech Republic), Filip RŮŽIČKA (203 Czech Republic, belonging to the institution) and Karel SLAIS (203 Czech Republic)

Edition

ACS INFECTIOUS DISEASES, WASHINGTON, AMER CHEMICAL SOC, 2019, 2373-8227

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10606 Microbiology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 4.614

RIV identification code

RIV/00216224:14110/19:00108321

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1021/acsinfecdis.9b00045

UT WoS

000480672600009

Keywords in English

uropathogens; high level of albumin; red nonionogenic tenside; preconcentration and preseparation; isoelectric focusing; matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

Abstract

V originále

Cellulose-based preparative isoelectric focusing was used for preseparation and concentration of uropathogens Staphylococcus aureus, Escherichia coli, Enterococcus faecalis, Staphylococcus epidermidis, Candida albicans, and Candida parapsilosis in a urine sample containing a high concentration of human serum albumin. For the visibility of the colorless microbial zones in the separation medium, the microbial cells were labeled with red nonionogenic tenside (1-[[4-(phenylazo)-phenyl]azo]-2-hydroxy-3-naphthoic acid polyethylene glycol ester, PAPAN). A very short incubation time, about 2 min, was sufficient for the adsorption of 0.001% (w/v) PAPAN onto the cell surface at the optimized conditions. As low as 10(3) cells of E. coli (pI 4.6) resuspended in 100 mu L of urine sample and spiked with 0.1 mg mL(-1) of human serum albumin (pI 4.8) were successfully preseparated and concentrated using this method. Because the pI values of the labeled microorganisms remained unchanged, the focused red zones of microbial cells were collected from the separation media and further analyzed by either capillary isoelectric focusing or matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The viability of the cells extracted from the collected zones was also confirmed. The proposed method provides reliable, relatively fast, and cost-effective identification of uropathogens in urine specimens with a high level of albumin.

Links

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

MUNI/A/1189/2018, interní kód MU

Name: Původci nozokomiálních nákaz a možnosti jejich diagnostiky a terapie (Acronym: PNNMDT)

Investor: Masaryk University, Category A

NV16-29916A, research and development project

Name: Využití bakteriofágů v léčbě nozokomiálních infekcí spojených s multirezistencí či tvorbou biofilmu

VI20172020069, research and development project

Name: Vývoj moderních instrumentálních metod pro rychlou detekci a identifikaci vybraných B-agens a toxinů (Acronym: VMIMRDIBT)

Investor: Ministry of the Interior of the CR

Citovat

HORKA, Marie, Jiri SALPLACHTA, Filip RŮŽIČKA and Karel SLAIS. Utilization of Red Nonionogenic Tenside Labeling, Isoelectric Focusing, and Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry in the Identification of Uropathogens in the Presence of a High Level of Albumin. ACS INFECTIOUS DISEASES. WASHINGTON: AMER CHEMICAL SOC, 2019, vol. 5, No 8, p. 1348-1356. ISSN 2373-8227. Available from: https://dx.doi.org/10.1021/acsinfecdis.9b00045.

@article{1623016,
   author = {Horka, Marie and Salplachta, Jiri and Růžička, Filip and Slais, Karel},
   article_location = {WASHINGTON},
   article_number = {8},
   doi = {http://dx.doi.org/10.1021/acsinfecdis.9b00045},
   keywords = {uropathogens; high level of albumin; red nonionogenic tenside; preconcentration and preseparation; isoelectric focusing; matrix-assisted laser desorption/ionization time-of-flight mass spectrometry},
   language = {eng},
   issn = {2373-8227},
   journal = {ACS INFECTIOUS DISEASES},
   title = {Utilization of Red Nonionogenic Tenside Labeling, Isoelectric Focusing, and Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry in the Identification of Uropathogens in the Presence of a High Level of Albumin},
   url = {http://dx.doi.org/10.1021/acsinfecdis.9b00045},
   volume = {5},
   year = {2019}
}

TY  - JOUR
ID  - 1623016
AU  - Horka, Marie - Salplachta, Jiri - Růžička, Filip - Slais, Karel
PY  - 2019
TI  - Utilization of Red Nonionogenic Tenside Labeling, Isoelectric Focusing, and Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry in the Identification of Uropathogens in the Presence of a High Level of Albumin
JF  - ACS INFECTIOUS DISEASES
VL  - 5
IS  - 8
SP  - 1348-1356
EP  - 1348-1356
PB  - AMER CHEMICAL SOC
SN  - 23738227
KW  - uropathogens
KW  - high level of albumin
KW  - red nonionogenic tenside
KW  - preconcentration and preseparation
KW  - isoelectric focusing
KW  - matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
UR  - http://dx.doi.org/10.1021/acsinfecdis.9b00045
L2  - http://dx.doi.org/10.1021/acsinfecdis.9b00045
N2  - Cellulose-based preparative isoelectric focusing was used for preseparation and concentration of uropathogens Staphylococcus aureus, Escherichia coli, Enterococcus faecalis, Staphylococcus epidermidis, Candida albicans, and Candida parapsilosis in a urine sample containing a high concentration of human serum albumin. For the visibility of the colorless microbial zones in the separation medium, the microbial cells were labeled with red nonionogenic tenside (1-[[4-(phenylazo)-phenyl]azo]-2-hydroxy-3-naphthoic acid polyethylene glycol ester, PAPAN). A very short incubation time, about 2 min, was sufficient for the adsorption of 0.001% (w/v) PAPAN onto the cell surface at the optimized conditions. As low as 10(3) cells of E. coli (pI 4.6) resuspended in 100 mu L of urine sample and spiked with 0.1 mg mL(-1) of human serum albumin (pI 4.8) were successfully preseparated and concentrated using this method. Because the pI values of the labeled microorganisms remained unchanged, the focused red zones of microbial cells were collected from the separation media and further analyzed by either capillary isoelectric focusing or matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The viability of the cells extracted from the collected zones was also confirmed. The proposed method provides reliable, relatively fast, and cost-effective identification of uropathogens in urine specimens with a high level of albumin.
ER  -

HORKA, Marie, Jiri SALPLACHTA, Filip RŮŽIČKA and Karel SLAIS. Utilization of Red Nonionogenic Tenside Labeling, Isoelectric Focusing, and Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry in the Identification of Uropathogens in the Presence of a High Level of Albumin. \textit{ACS INFECTIOUS DISEASES}. WASHINGTON: AMER CHEMICAL SOC, 2019, vol.~5, No~8, p.~1348-1356. ISSN~2373-8227. Available from: https://dx.doi.org/10.1021/acsinfecdis.9b00045.

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