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@article{1623818,
author = {Salát, Jiří and Mikulášek, Kamil and Larralde, Osmany and Pokorná Formanová, Petra and Chrdle, Aleš and Haviernik, Jan and Elsterová, Jana and Teislerová, Dana and Palus, Martin and Eyer, Luděk and Zdráhal, Zbyněk and Petrik, Juraj and Růžek, Daniel},
article_location = {Basel},
article_number = {1},
doi = {http://dx.doi.org/10.3390/vaccines8010081},
keywords = {NS1; flavivirus; tick-borne encephalitis; vaccination; vaccine},
language = {eng},
issn = {2076-393X},
journal = {Vaccines},
title = {Tick-Borne Encephalitis Virus Vaccines Contain Non-Structural Protein 1 Antigen and may Elicit NS1-Specific Antibody Responses in Vaccinated Individuals},
url = {https://www.mdpi.com/2076-393X/8/1/81},
volume = {8},
year = {2020}
}
TY - JOUR
ID - 1623818
AU - Salát, Jiří - Mikulášek, Kamil - Larralde, Osmany - Pokorná Formanová, Petra - Chrdle, Aleš - Haviernik, Jan - Elsterová, Jana - Teislerová, Dana - Palus, Martin - Eyer, Luděk - Zdráhal, Zbyněk - Petrik, Juraj - Růžek, Daniel
PY - 2020
TI - Tick-Borne Encephalitis Virus Vaccines Contain Non-Structural Protein 1 Antigen and may Elicit NS1-Specific Antibody Responses in Vaccinated Individuals
JF - Vaccines
VL - 8
IS - 1
SP - 1-13
EP - 1-13
PB - MDPI
SN - 2076393X
KW - NS1
KW - flavivirus
KW - tick-borne encephalitis
KW - vaccination
KW - vaccine
UR - https://www.mdpi.com/2076-393X/8/1/81
L2 - https://www.mdpi.com/2076-393X/8/1/81
N2 - Vaccination against tick-borne encephalitis (TBE) is based on the use of formalin-inactivated, culture-derived whole-virus vaccines. Immune response following vaccination is primarily directed to the viral envelope (E) protein, the major viral surface antigen. In Europe, two TBE vaccines are available in adult and pediatric formulations, namely FSME-IMMUN (R) (Pfizer) and Encepur((R)) (GlaxoSmithKline). Herein, we analyzed the content of these vaccines using mass spectrometry (MS). The MS analysis revealed that the Encepur vaccine contains not only proteins of the whole virus particle, but also viral non-structural protein 1 (NS1). MS analysis of the FSME-IMMUN vaccine failed due to the high content of human serum albumin used as a stabilizer in the vaccine. However, the presence of NS1 in FSME-IMMUN was confirmed by immunization of mice with six doses of this vaccine, which led to a robust anti-NS1 antibody response. NS1-specific Western blot analysis also detected anti-NS1 antibodies in sera of humans who received multiple doses of either of these two vaccines; however, most vaccinees who received <= 3 doses were negative for NS1-specific antibodies. The contribution of NS1-specific antibodies to protection against TBE was demonstrated by immunization of mice with purified NS1 antigen, which led to a significant (p < 0.01) prolongation of the mean survival time after lethal virus challenge. This indicates that stimulation of anti-NS1 immunity by the TBE vaccines may increase their protective effect.
ER -
SALÁT, Jiří, Kamil MIKULÁŠEK, Osmany LARRALDE, Petra POKORNÁ FORMANOVÁ, Aleš CHRDLE, Jan HAVIERNIK, Jana ELSTEROVÁ, Dana TEISLEROVÁ, Martin PALUS, Luděk EYER, Zbyněk ZDRÁHAL, Juraj PETRIK and Daniel RŮŽEK. Tick-Borne Encephalitis Virus Vaccines Contain Non-Structural Protein 1 Antigen and may Elicit NS1-Specific Antibody Responses in Vaccinated Individuals. \textit{Vaccines}. Basel: MDPI, 2020, vol.~8, No~1, p.~1-13. ISSN~2076-393X. Available from: https://dx.doi.org/10.3390/vaccines8010081.
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