Detailed Information on Publication Record
2019
Novel markers of human ovarian granulosa cell differentiation toward osteoblast lineage: A microarray approach
BRAZERT, Maciej, Wieslawa KRANC, Piotr CELICHOWSKI, Katarzyna OZEGOWSKA, Joanna BUDNA-TUKAN et. al.Basic information
Original name
Novel markers of human ovarian granulosa cell differentiation toward osteoblast lineage: A microarray approach
Authors
BRAZERT, Maciej (616 Poland), Wieslawa KRANC (616 Poland), Piotr CELICHOWSKI (616 Poland), Katarzyna OZEGOWSKA (616 Poland), Joanna BUDNA-TUKAN (616 Poland), Michal JEŠETA (203 Czech Republic, belonging to the institution), Leszek PAWELCZYK (616 Poland), Malgorzata BRUSKA (616 Poland), Maciej ZABEL (616 Poland), Michal NOWICKI (616 Poland) and Bartosz KEMPISTY (616 Poland, guarantor)
Edition
Molecular Medicine Reports, ATHENS, SPANDIDOS PUBL LTD, 2019, 1791-2997
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
30214 Obstetrics and gynaecology
Country of publisher
Greece
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 2.100
RIV identification code
RIV/00216224:14110/19:00112986
Organization unit
Faculty of Medicine
UT WoS
000491393100046
Keywords in English
ovarian granulosa; osteoblast; differentiation; microarray; in vitro
Tags
International impact, Reviewed
Změněno: 18/2/2020 11:23, Mgr. Tereza Miškechová
Abstract
V originále
Under physiological conditions, human ovarian granulosa cells (GCs), are responsible for a number of processes associated with folliculogenesis and oogenesis. The primary functions of GCs in the individual phases of follicle growth are: Hormone production in response to follicle stimulating hormone (FSH), induction of ovarian follicle atresia through specific molecular markers and production of nexus cellular connections for communication with the oocyte. In recent years, interest in obtaining stem cells from particular tissues, including the ovary, has increased. Special attention has been paid to the novel properties of GCs during long-term in vitro culture. It has been demonstrated that the usually recycled material in the form of follicular fluid can be a source of cells with stem-like properties. The study group consisted of patients enrolled in the in vitro fertilization procedure. Total RNA was isolated from GCs at 4 time points (after 1, 7, 15 and 30 days of culture) and was used for microarray expression analysis (Affymetrix (R) Human HgU 219 Array). The expression of 22,480 transcripts was examined. The selection of significantly altered genes was based on a P-value <0.05 and expression higher than two-fold. The leucine rich repeat containing 17, collagen type I alpha 1 chain, bone morphogenetic protein 4, twist family bHLH transcription factor 1, insulin like growth factor binding protein 5, GLI family zinc finger 2 and collagen triple helix repeat containing genes exhibited the highest changes in expression. Reverse-transcription-quantitative PCR was performed to validate the results obtained in the analysis of expression microarrays. The direction of expression changes was validated in the majority of cases. The presented results indicated that GCs have the potential of cells that can differentiate towards osteoblasts in long-term in vitro culture conditions. Increased expression of genes associated with the osteogenesis process suggests a potential for uninduced change of GC properties towards the osteoblast phenotype. The present study, therefore, suggests that GCs may become an excellent starting material in obtaining stable osteoblast cultures. GCs differentiated towards osteoblasts may be used in regenerative and reconstructive medicine in the future.