Phospho-Protein Arrays as Effective Tools for Screening Possible Targets for Kinase Inhibitors and ...

@article{1623977,
   author = {Neradil, Jakub and Kýr, Michal and Polášková, Kristýna and Křen, Leoš and Macigová, Petra and Škoda, Jan and Štěrba, Jaroslav and Veselská, Renata},
   article_location = {Lausanne},
   article_number = {SEP 20},
   doi = {http://dx.doi.org/10.3389/fonc.2019.00930},
   keywords = {low-molecular-weight inhibitors; pediatric solid tumors; phospho-protein arrays; phosphorylation profiling; receptor tyrosin kinases; signal transduction},
   language = {eng},
   issn = {2234-943X},
   journal = {Frontiers in Oncology},
   title = {Phospho-Protein Arrays as Effective Tools for Screening Possible Targets for Kinase Inhibitors and Their Use in Precision Pediatric Oncology},
   url = {https://www.frontiersin.org/articles/10.3389/fonc.2019.00930/full},
   volume = {9},
   year = {2019}
}

TY  - JOUR
ID  - 1623977
AU  - Neradil, Jakub - Kýr, Michal - Polášková, Kristýna - Křen, Leoš - Macigová, Petra - Škoda, Jan - Štěrba, Jaroslav - Veselská, Renata
PY  - 2019
TI  - Phospho-Protein Arrays as Effective Tools for Screening Possible Targets for Kinase Inhibitors and Their Use in Precision Pediatric Oncology
JF  - Frontiers in Oncology
VL  - 9
IS  - SEP 20
SP  - 1-11
EP  - 1-11
PB  - Frontiers Media SA
SN  - 2234943X
KW  - low-molecular-weight inhibitors
KW  - pediatric solid tumors
KW  - phospho-protein arrays
KW  - phosphorylation profiling
KW  - receptor tyrosin kinases
KW  - signal transduction
UR  - https://www.frontiersin.org/articles/10.3389/fonc.2019.00930/full
L2  - https://www.frontiersin.org/articles/10.3389/fonc.2019.00930/full
N2  - The specific targeting of signal transduction by low-molecular-weight inhibitors or monoclonal antibodies represents a very promising personalized treatment strategy in pediatric oncology. In this study, we present the successful and clinically relevant use of commercially available phospho-protein arrays for analyses of the phosphorylation profiles of a broad spectrum of receptor tyrosine kinases and their downstream signaling proteins in tumor tissue samples. Although these arrays were made for research purposes on human biological samples, they have already been used by several authors to profile various tumor types. Our study performed a systematic analysis of the advantages and pitfalls of the use of this method for personalized clinical medicine. In certain clinical cases and their series, we demonstrated the important aspects of data processing and evaluation, the use of phospho-protein arrays for single sample and serial sample analyses, and the validation of obtained results by immunohistochemistry, as well as the possibilities of this method for the hierarchical clustering of pediatric solid tumors. Our results clearly show that phospho-protein arrays are apparently useful for the clinical consideration of druggable molecular targets within a specific tumor. Thus, their potential validation for diagnostic purposes may substantially improve the personalized approach in the treatment of relapsed or refractory solid tumors.
ER  - 

NERADIL, Jakub, Michal KÝR, Kristýna POLÁŠKOVÁ, Leoš KŘEN, Petra MACIGOVÁ, Jan ŠKODA, Jaroslav ŠTĚRBA and Renata VESELSKÁ. Phospho-Protein Arrays as Effective Tools for Screening Possible Targets for Kinase Inhibitors and Their Use in Precision Pediatric Oncology. \textit{Frontiers in Oncology}. Lausanne: Frontiers Media SA, 2019, vol.~9, SEP 20, p.~1-11. ISSN~2234-943X. Available from: https://dx.doi.org/10.3389/fonc.2019.00930.