BUDNA-TUKAN, Joanna, Agata SWIATLY-BLASZKIEWICZ, Piotr CELICHOWSKI, Sandra KALUZNA, Aneta KONWERSKA, Patrycja SUJKA-KORDOWSKA, Maurycy JANKOWSKI, Magdalena KULUS, Michal JEŠETA, Hanna PIOTROWSKA-KEMPISTY, Malgorzata JOZKOWIAK, Pawel ANTOSIK, Dorota BUKOWSKA, Mariusz T. SKOWRONSKI, Jan MATYSIAK, Michal NOWICKI and Bartosz KEMPISTY. "Biological Adhesion" Is a Significantly Regulated Molecular Process during Long-Term Primary In Vitro Culture of Oviductal Epithelial Cells (Oecs): A Transcriptomic and Proteomic Study. Online. International Journal of Molecular Sciences. Basel: Multidisciplinary Digital Publishing Institute, 2019, vol. 20, No 14, p. 1-18. ISSN 1422-0067. Available from: https://dx.doi.org/10.3390/ijms20143387. [citováno 2024-04-23]
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Basic information
Original name "Biological Adhesion" Is a Significantly Regulated Molecular Process during Long-Term Primary In Vitro Culture of Oviductal Epithelial Cells (Oecs): A Transcriptomic and Proteomic Study
Authors BUDNA-TUKAN, Joanna (616 Poland, guarantor), Agata SWIATLY-BLASZKIEWICZ (616 Poland), Piotr CELICHOWSKI (616 Poland), Sandra KALUZNA (616 Poland), Aneta KONWERSKA (616 Poland), Patrycja SUJKA-KORDOWSKA (616 Poland), Maurycy JANKOWSKI (616 Poland), Magdalena KULUS (616 Poland), Michal JEŠETA (203 Czech Republic, belonging to the institution), Hanna PIOTROWSKA-KEMPISTY (616 Poland), Malgorzata JOZKOWIAK (616 Poland), Pawel ANTOSIK (616 Poland), Dorota BUKOWSKA (616 Poland), Mariusz T. SKOWRONSKI (616 Poland), Jan MATYSIAK (616 Poland), Michal NOWICKI (616 Poland) and Bartosz KEMPISTY (616 Poland)
Edition International Journal of Molecular Sciences, Basel, Multidisciplinary Digital Publishing Institute, 2019, 1422-0067.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10608 Biochemistry and molecular biology
Country of publisher Switzerland
Confidentiality degree is not subject to a state or trade secret
WWW URL
Impact factor Impact factor: 4.556
RIV identification code RIV/00216224:14110/19:00112990
Organization unit Faculty of Medicine
Doi http://dx.doi.org/10.3390/ijms20143387
UT WoS 000480449300012
Keywords in English oviductal epithelial cells; biological adhesion; microarray; mass spectrometry; pig
Tags 14110411, rivok
Tags International impact, Reviewed
Changed by Changed by: Mgr. Tereza Miškechová, učo 341652. Changed: 21/7/2021 10:30.
Abstract
Oviductal epithelial cells (OECs) actively produce stimulating and protecting factors, favoring survival and viability of gametes and early embryos. The oviduct participates in the initial reproductive events, which strongly depends on adhesion. The analysis of differential gene expression in OECs, during long-term in vitro culture, enables recognition of new molecular markers regulating several processes, including "biological adhesion". Porcine oviducts were stained with hematoxylin and eosin, as well as with antibodies against epithelial markers. Then, OECs were long-term in vitro cultured and after 24 h, 7, 15, and 30 days of culture were subjected to transcriptomic and proteomic assays. Microarrays were employed to evaluate gene expression, with Matrix-assisted laser desorption/ionization-time of light (MALDI-TOF) mass spectrometry applied to determine the proteome. The results revealed proper morphology of the oviducts and typical epithelial structure of OECs during the culture. From the set of differentially expressed genes (DEGs), we have selected the 130 that encoded proteins detected by MALDI-TOF MS analysis. From this gene pool, 18 significantly enriched gene ontology biological processes (GO BP) terms were extracted. Among them we focused on genes belonging to "biological adhesion" GO BP. It is suggested that increased expression of studied genes can be attributed to the process of intensive secretion of substances that exhibit favorable influence on oviductal environment, which prime gametes adhesion and viability, fertilization, and early embryo journey.
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