Genes Involved in the Processes of Cell Proliferation,
Migration, Adhesion, and Tissue Development as New Potential
Markers of Porcine Granulosa Cellular Processes In Vitro: A
Microarray Approach

J 2019

Genes Involved in the Processes of Cell Proliferation, Migration, Adhesion, and Tissue Development as New Potential Markers of Porcine Granulosa Cellular Processes In Vitro: A Microarray Approach

OZEGOWSKA, Katarzyna, Maciej BRAZERT, Sylwia CIESIOLKA, Mariusz J. NAWROCKI, Wieslawa KRANC et. al.

Basic information

Original name

Genes Involved in the Processes of Cell Proliferation, Migration, Adhesion, and Tissue Development as New Potential Markers of Porcine Granulosa Cellular Processes In Vitro: A Microarray Approach

Authors

OZEGOWSKA, Katarzyna (616 Poland), Maciej BRAZERT (616 Poland), Sylwia CIESIOLKA (616 Poland), Mariusz J. NAWROCKI (616 Poland), Wieslawa KRANC (616 Poland), Piotr CELICHOWSKI (616 Poland), Maurycy JANKOWSKI (616 Poland), Artur BRYJA (616 Poland), Michal JEŠETA (203 Czech Republic, belonging to the institution), Pawel ANTOSIK (616 Poland), Dorota BUKOWSKA (616 Poland), Mariusz T. SKOWRONSKI (616 Poland), Malgorzata BRUSKA (616 Poland), Leszek PAWELCZYK (616 Poland), Maciej ZABEL (616 Poland), Michal NOWICKI (616 Poland) and Bartosz KEMPISTY (616 Poland, guarantor)

Edition

DNA and Cell Biology, New York, Mary Ann Liebert Inc. 2019, 1044-5498

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10601 Cell biology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 3.314

RIV identification code

RIV/00216224:14110/19:00112992

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1089/dna.2018.4467

UT WoS

000473437200006

Keywords in English

porcine granulosa cells; In Vitro Maturation (IVM); cellular migration; proliferation; adhesion

Abstract

V originále

Proper course of folliculogenesis and oogenesis have an enormous impact on female fertility. Both processes take place in the ovary and involve not only the maturing germ cell, but also few types of somatic cells that assist the ovarian processes and mediate the dialog with the oocyte. These cells, granulosa and theca, are heavily involved in essential reproductive processes, such as ovulation, fertilization, and embryo implantation. In this study, we have used the expressive microarray approach to analyze the transcriptome of porcine granulosa cells, during short-term in vitro culture. We have further selected differentially expressed gene ontologies, involved in cell proliferation, migration, adhesion, and tissue development, namely, "cell-cell adhesion," "cell motility," "cell proliferation," "tissue development," and "tissue migration" to screen them for the possibility of discovery of new markers of those processes. A total of 303 genes, expression of which varied significantly in different culture periods and belonged to the analyzed ontology groups, were detected, of which 15 that varied the most (between 0 and 48 h of culture) were selected for validation. As the validation confirmed the transcriptomic patterns, 10 genes of biggest changes in expression (CAV1, IGFBP5, ITGB3, FN1, ITGA2, LAMB1, POSTN, FAM83D, KIF14, and CDK1) were analyzed, described, and referred to the context of the study, with the most promising new markers and further proof for the viability of the currently recognized ones detailed. Overall, the study provided valuable insight into the molecular functioning of in vitro granulosa cell cultures.

Citovat

OZEGOWSKA, Katarzyna, Maciej BRAZERT, Sylwia CIESIOLKA, Mariusz J. NAWROCKI, Wieslawa KRANC, Piotr CELICHOWSKI, Maurycy JANKOWSKI, Artur BRYJA, Michal JEŠETA, Pawel ANTOSIK, Dorota BUKOWSKA, Mariusz T. SKOWRONSKI, Malgorzata BRUSKA, Leszek PAWELCZYK, Maciej ZABEL, Michal NOWICKI and Bartosz KEMPISTY. Genes Involved in the Processes of Cell Proliferation, Migration, Adhesion, and Tissue Development as New Potential Markers of Porcine Granulosa Cellular Processes In Vitro: A Microarray Approach. DNA and Cell Biology. New York: Mary Ann Liebert Inc., 2019, vol. 38, No 6, p. 549-560. ISSN 1044-5498. Available from: https://dx.doi.org/10.1089/dna.2018.4467.

@article{1624056,
   author = {Ozegowska, Katarzyna and Brazert, Maciej and Ciesiolka, Sylwia and Nawrocki, Mariusz J. and Kranc, Wieslawa and Celichowski, Piotr and Jankowski, Maurycy and Bryja, Artur and Ješeta, Michal and Antosik, Pawel and Bukowska, Dorota and Skowronski, Mariusz T. and Bruska, Malgorzata and Pawelczyk, Leszek and Zabel, Maciej and Nowicki, Michal and Kempisty, Bartosz},
   article_location = {New York},
   article_number = {6},
   doi = {http://dx.doi.org/10.1089/dna.2018.4467},
   keywords = {porcine granulosa cells; In Vitro Maturation (IVM); cellular migration; proliferation; adhesion},
   language = {eng},
   issn = {1044-5498},
   journal = {DNA and Cell Biology},
   title = {Genes Involved in the Processes of Cell Proliferation, Migration, Adhesion, and Tissue Development as New Potential Markers of Porcine Granulosa Cellular Processes In Vitro: A Microarray Approach},
   url = {http://dx.doi.org/10.1089/dna.2018.4467},
   volume = {38},
   year = {2019}
}

TY  - JOUR
ID  - 1624056
AU  - Ozegowska, Katarzyna - Brazert, Maciej - Ciesiolka, Sylwia - Nawrocki, Mariusz J. - Kranc, Wieslawa - Celichowski, Piotr - Jankowski, Maurycy - Bryja, Artur - Ješeta, Michal - Antosik, Pawel - Bukowska, Dorota - Skowronski, Mariusz T. - Bruska, Malgorzata - Pawelczyk, Leszek - Zabel, Maciej - Nowicki, Michal - Kempisty, Bartosz
PY  - 2019
TI  - Genes Involved in the Processes of Cell Proliferation, Migration, Adhesion, and Tissue Development as New Potential Markers of Porcine Granulosa Cellular Processes In Vitro: A Microarray Approach
JF  - DNA and Cell Biology
VL  - 38
IS  - 6
SP  - 549-560
EP  - 549-560
PB  - Mary Ann Liebert Inc.
SN  - 10445498
KW  - porcine granulosa cells
KW  - In Vitro Maturation (IVM)
KW  - cellular migration
KW  - proliferation
KW  - adhesion
UR  - http://dx.doi.org/10.1089/dna.2018.4467
L2  - http://dx.doi.org/10.1089/dna.2018.4467
N2  - Proper course of folliculogenesis and oogenesis have an enormous impact on female fertility. Both processes take place in the ovary and involve not only the maturing germ cell, but also few types of somatic cells that assist the ovarian processes and mediate the dialog with the oocyte. These cells, granulosa and theca, are heavily involved in essential reproductive processes, such as ovulation, fertilization, and embryo implantation. In this study, we have used the expressive microarray approach to analyze the transcriptome of porcine granulosa cells, during short-term in vitro culture. We have further selected differentially expressed gene ontologies, involved in cell proliferation, migration, adhesion, and tissue development, namely, "cell-cell adhesion," "cell motility," "cell proliferation," "tissue development," and "tissue migration" to screen them for the possibility of discovery of new markers of those processes. A total of 303 genes, expression of which varied significantly in different culture periods and belonged to the analyzed ontology groups, were detected, of which 15 that varied the most (between 0 and 48 h of culture) were selected for validation. As the validation confirmed the transcriptomic patterns, 10 genes of biggest changes in expression (CAV1, IGFBP5, ITGB3, FN1, ITGA2, LAMB1, POSTN, FAM83D, KIF14, and CDK1) were analyzed, described, and referred to the context of the study, with the most promising new markers and further proof for the viability of the currently recognized ones detailed. Overall, the study provided valuable insight into the molecular functioning of in vitro granulosa cell cultures.
ER  -

OZEGOWSKA, Katarzyna, Maciej BRAZERT, Sylwia CIESIOLKA, Mariusz J. NAWROCKI, Wieslawa KRANC, Piotr CELICHOWSKI, Maurycy JANKOWSKI, Artur BRYJA, Michal JEŠETA, Pawel ANTOSIK, Dorota BUKOWSKA, Mariusz T. SKOWRONSKI, Malgorzata BRUSKA, Leszek PAWELCZYK, Maciej ZABEL, Michal NOWICKI and Bartosz KEMPISTY. Genes Involved in the Processes of Cell Proliferation, Migration, Adhesion, and Tissue Development as New Potential Markers of Porcine Granulosa Cellular Processes In Vitro: A Microarray Approach. \textit{DNA and Cell Biology}. New York: Mary Ann Liebert Inc., 2019, vol.~38, No~6, p.~549-560. ISSN~1044-5498. Available from: https://dx.doi.org/10.1089/dna.2018.4467.

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