Genes Involved in the Processes of Cell Proliferation, Migration, Adhesion, and Tissue Development as New Potential Markers of Porcine Granulosa Cellular Processes In Vitro: A Microarray Approach

OZEGOWSKA, Katarzyna, Maciej BRAZERT, Sylwia CIESIOLKA, Mariusz J. NAWROCKI, Wieslawa KRANC, Piotr CELICHOWSKI, Maurycy JANKOWSKI, Artur BRYJA, Michal JEŠETA, Pawel ANTOSIK, Dorota BUKOWSKA, Mariusz T. SKOWRONSKI, Malgorzata BRUSKA, Leszek PAWELCZYK, Maciej ZABEL, Michal NOWICKI and Bartosz KEMPISTY. Genes Involved in the Processes of Cell Proliferation, Migration, Adhesion, and Tissue Development as New Potential Markers of Porcine Granulosa Cellular Processes In Vitro: A Microarray Approach. DNA and Cell Biology. New York: Mary Ann Liebert Inc., 2019, vol. 38, No 6, p. 549-560. ISSN 1044-5498. Available from: https://dx.doi.org/10.1089/dna.2018.4467.

Basic information

• Original name: Genes Involved in the Processes of Cell Proliferation, Migration, Adhesion, and Tissue Development as New Potential Markers of Porcine Granulosa Cellular Processes In Vitro: A Microarray Approach
• Authors: OZEGOWSKA, Katarzyna (616 Poland), Maciej BRAZERT (616 Poland), Sylwia CIESIOLKA (616 Poland), Mariusz J. NAWROCKI (616 Poland), Wieslawa KRANC (616 Poland), Piotr CELICHOWSKI (616 Poland), Maurycy JANKOWSKI (616 Poland), Artur BRYJA (616 Poland), Michal JEŠETA (203 Czech Republic, belonging to the institution), Pawel ANTOSIK (616 Poland), Dorota BUKOWSKA (616 Poland), Mariusz T. SKOWRONSKI (616 Poland), Malgorzata BRUSKA (616 Poland), Leszek PAWELCZYK (616 Poland), Maciej ZABEL (616 Poland), Michal NOWICKI (616 Poland) and Bartosz KEMPISTY (616 Poland, guarantor).
• Edition: DNA and Cell Biology, New York, Mary Ann Liebert Inc. 2019, 1044-5498.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 10601 Cell biology
• Country of publisher: United States of America
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• Impact factor: Impact factor: 3.314
• RIV identification code: RIV/00216224:14110/19:00112992
• Organization unit: Faculty of Medicine
• Doi: http://dx.doi.org/10.1089/dna.2018.4467
• UT WoS: 000473437200006
• Keywords in English: porcine granulosa cells; In Vitro Maturation (IVM); cellular migration; proliferation; adhesion
• Tags: 14110411, rivok
• Tags: International impact, Reviewed

Abstract

Proper course of folliculogenesis and oogenesis have an enormous impact on female fertility. Both processes take place in the ovary and involve not only the maturing germ cell, but also few types of somatic cells that assist the ovarian processes and mediate the dialog with the oocyte. These cells, granulosa and theca, are heavily involved in essential reproductive processes, such as ovulation, fertilization, and embryo implantation. In this study, we have used the expressive microarray approach to analyze the transcriptome of porcine granulosa cells, during short-term in vitro culture. We have further selected differentially expressed gene ontologies, involved in cell proliferation, migration, adhesion, and tissue development, namely, "cell-cell adhesion," "cell motility," "cell proliferation," "tissue development," and "tissue migration" to screen them for the possibility of discovery of new markers of those processes. A total of 303 genes, expression of which varied significantly in different culture periods and belonged to the analyzed ontology groups, were detected, of which 15 that varied the most (between 0 and 48 h of culture) were selected for validation. As the validation confirmed the transcriptomic patterns, 10 genes of biggest changes in expression (CAV1, IGFBP5, ITGB3, FN1, ITGA2, LAMB1, POSTN, FAM83D, KIF14, and CDK1) were analyzed, described, and referred to the context of the study, with the most promising new markers and further proof for the viability of the currently recognized ones detailed. Overall, the study provided valuable insight into the molecular functioning of in vitro granulosa cell cultures.