EXPRESSION OF PTX3, HAS2 AND TNFAIP6 GENES IN RELATION TO
REAL-TIME PROLIFERATION OF PORCINE ENDOMETRIAL LUMINAL
EPITHELIAL CELLS IN PRIMARY CULTIVATION MODEL

J 2019

EXPRESSION OF PTX3, HAS2 AND TNFAIP6 GENES IN RELATION TO REAL-TIME PROLIFERATION OF PORCINE ENDOMETRIAL LUMINAL EPITHELIAL CELLS IN PRIMARY CULTIVATION MODEL

WOJTANOWICZ-MARKIEWICZ, K., I. KOCHEROVA, Michal JEŠETA, H. PIOTROWSKA-KEMPISTY, K. P. BRUSSOW et. al.

Basic information

Original name

EXPRESSION OF PTX3, HAS2 AND TNFAIP6 GENES IN RELATION TO REAL-TIME PROLIFERATION OF PORCINE ENDOMETRIAL LUMINAL EPITHELIAL CELLS IN PRIMARY CULTIVATION MODEL

Authors

WOJTANOWICZ-MARKIEWICZ, K. (616 Poland), I. KOCHEROVA (616 Poland), Michal JEŠETA (203 Czech Republic, belonging to the institution), H. PIOTROWSKA-KEMPISTY (616 Poland), K. P. BRUSSOW (616 Poland), M. T. SKOWRONSKI (616 Poland), M. BRUSKA (616 Poland), D. BUKOWSKA (616 Poland), M. NOWICKI (616 Poland), B. KEMPISTY (616 Poland, guarantor) and P. ANTOSIK (616 Poland)

Edition

Journal of biological regulators and homeostatic agents, Silva Marina, Biolife SAS, 2019, 0393-974X

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30202 Endocrinology and metabolism

Country of publisher

Italy

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 1.506

RIV identification code

RIV/00216224:14110/19:00112993

Organization unit

Faculty of Medicine

UT WoS

000484232000004

Keywords in English

pig; primary culture; genes expression; luminal epithelial cells

Abstract

V originále

Endometrial cells undergo very specific changes associated with reproductive processes. Cells prepare for embryo development by increasing their volume. Then, if fertilization fails, endometrial cells are liable for apoptosis, preparing new cells that are ready for subsequent processes related to the possibility of embryo implantation and the development of pregnancy. PTX3 and TNFAIP6 are absent or reduced in cultured COCs, resulting in a functional change in COC in vitro. In this work, we want to check how PTX3, HAS2 and TNFAIP6 behave in luminal epithelium primary cell culture. Cells obtained during slaughter from porcine specimens were cultured primarily in vitro for 7 days. Their proliferation patterns were then analysed using RTCA, with the expression of genes of interest evaluated with the use of immunofluorescence and RT-qPCR. The results of these changes in the expression of the genes of interest were analysed on each of the seven days of the porcine luminal primary cell culture. Our study showed the increased level of PTX3, HAS2 and TNFAIP6 expression at the same hours of primary culture. Rt-qPCR showed a higher level of expression of the PTX3 gene in the first 72 h, at the end of the lag phase (in the phase of stasis in which the cells adapt to the new environment and often die). In contrast, TNFAIP6 expression increases about 96 hours when the cells are in the full log phase (logarithmic phase growth) and continue this trend in the plateau phase. We did not observe such drastic changes in the HAS2 expression pattern, which leads us to hypothesize that PTX3 and TNFAIP6 are designed to maintain a constant level of HAS2 in the cell throughout its lifetime. The obtained results could become a point of reference for further in vivo and clinical research.

Citovat

WOJTANOWICZ-MARKIEWICZ, K., I. KOCHEROVA, Michal JEŠETA, H. PIOTROWSKA-KEMPISTY, K. P. BRUSSOW, M. T. SKOWRONSKI, M. BRUSKA, D. BUKOWSKA, M. NOWICKI, B. KEMPISTY and P. ANTOSIK. EXPRESSION OF PTX3, HAS2 AND TNFAIP6 GENES IN RELATION TO REAL-TIME PROLIFERATION OF PORCINE ENDOMETRIAL LUMINAL EPITHELIAL CELLS IN PRIMARY CULTIVATION MODEL. Journal of biological regulators and homeostatic agents. Silva Marina: Biolife SAS, 2019, vol. 33, No 3, p. 675-685. ISSN 0393-974X.

@article{1624077,
   author = {WojtanowiczandMarkiewicz, K. and Kocherova, I. and Ješeta, Michal and PiotrowskaandKempisty, H. and Brussow, K. P. and Skowronski, M. T. and Bruska, M. and Bukowska, D. and Nowicki, M. and Kempisty, B. and Antosik, P.},
   article_location = {Silva Marina},
   article_number = {3},
   keywords = {pig; primary culture; genes expression; luminal epithelial cells},
   language = {eng},
   issn = {0393-974X},
   journal = {Journal of biological regulators and homeostatic agents},
   title = {EXPRESSION OF PTX3, HAS2 AND TNFAIP6 GENES IN RELATION TO REAL-TIME PROLIFERATION OF PORCINE ENDOMETRIAL LUMINAL EPITHELIAL CELLS IN PRIMARY CULTIVATION MODEL},
   url = {https://www.ncbi.nlm.nih.gov/pubmed/31189490},
   volume = {33},
   year = {2019}
}

TY  - JOUR
ID  - 1624077
AU  - Wojtanowicz-Markiewicz, K. - Kocherova, I. - Ješeta, Michal - Piotrowska-Kempisty, H. - Brussow, K. P. - Skowronski, M. T. - Bruska, M. - Bukowska, D. - Nowicki, M. - Kempisty, B. - Antosik, P.
PY  - 2019
TI  - EXPRESSION OF PTX3, HAS2 AND TNFAIP6 GENES IN RELATION TO REAL-TIME PROLIFERATION OF PORCINE ENDOMETRIAL LUMINAL EPITHELIAL CELLS IN PRIMARY CULTIVATION MODEL
JF  - Journal of biological regulators and homeostatic agents
VL  - 33
IS  - 3
SP  - 675-685
EP  - 675-685
PB  - Biolife SAS
SN  - 0393974X
KW  - pig
KW  - primary culture
KW  - genes expression
KW  - luminal epithelial cells
UR  - https://www.ncbi.nlm.nih.gov/pubmed/31189490
L2  - https://www.ncbi.nlm.nih.gov/pubmed/31189490
N2  - Endometrial cells undergo very specific changes associated with reproductive processes. Cells prepare for embryo development by increasing their volume. Then, if fertilization fails, endometrial cells are liable for apoptosis, preparing new cells that are ready for subsequent processes related to the possibility of embryo implantation and the development of pregnancy. PTX3 and TNFAIP6 are absent or reduced in cultured COCs, resulting in a functional change in COC in vitro. In this work, we want to check how PTX3, HAS2 and TNFAIP6 behave in luminal epithelium primary cell culture. Cells obtained during slaughter from porcine specimens were cultured primarily in vitro for 7 days. Their proliferation patterns were then analysed using RTCA, with the expression of genes of interest evaluated with the use of immunofluorescence and RT-qPCR. The results of these changes in the expression of the genes of interest were analysed on each of the seven days of the porcine luminal primary cell culture. Our study showed the increased level of PTX3, HAS2 and TNFAIP6 expression at the same hours of primary culture. Rt-qPCR showed a higher level of expression of the PTX3 gene in the first 72 h, at the end of the lag phase (in the phase of stasis in which the cells adapt to the new environment and often die). In contrast, TNFAIP6 expression increases about 96 hours when the cells are in the full log phase (logarithmic phase growth) and continue this trend in the plateau phase. We did not observe such drastic changes in the HAS2 expression pattern, which leads us to hypothesize that PTX3 and TNFAIP6 are designed to maintain a constant level of HAS2 in the cell throughout its lifetime. The obtained results could become a point of reference for further in vivo and clinical research.
ER  -

WOJTANOWICZ-MARKIEWICZ, K., I. KOCHEROVA, Michal JEŠETA, H. PIOTROWSKA-KEMPISTY, K. P. BRUSSOW, M. T. SKOWRONSKI, M. BRUSKA, D. BUKOWSKA, M. NOWICKI, B. KEMPISTY and P. ANTOSIK. EXPRESSION OF PTX3, HAS2 AND TNFAIP6 GENES IN RELATION TO REAL-TIME PROLIFERATION OF PORCINE ENDOMETRIAL LUMINAL EPITHELIAL CELLS IN PRIMARY CULTIVATION MODEL. \textit{Journal of biological regulators and homeostatic agents}. Silva Marina: Biolife SAS, 2019, vol.~33, No~3, p.~675-685. ISSN~0393-974X.

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