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@article{1624137,
author = {Kranc, Wieslawa and Brazert, Maciej and Celichowski, Piotr and Bryja, Artur and Nawrocki, Mariusz J. and Ozegowska, Katarzyna and Jankowski, Maurycy and Ješeta, Michal and Pawelczyk, Leszek and Breborowicz, Andrzej and Rachon, Dominik and Skowronski, Mariusz T. and Bruska, Malgorzata and Zabel, Maciej and Nowicki, Michal and Kempisty, Bartosz},
article_location = {ATHENS},
article_number = {3},
doi = {http://dx.doi.org/10.3892/mmr.2019.9837},
keywords = {human granulosa cells; in vitro culture; proliferation; differentiation; heart development},
language = {eng},
issn = {1791-2997},
journal = {Molecular Medicine Reports},
title = {"Heart development and morphogenesis' is a novel pathway for human ovarian granulosa cell differentiation during long-term in vitro cultivation-a microarray approach},
url = {https://www.ncbi.nlm.nih.gov/pubmed/30628715},
volume = {19},
year = {2019}
}
TY - JOUR
ID - 1624137
AU - Kranc, Wieslawa - Brazert, Maciej - Celichowski, Piotr - Bryja, Artur - Nawrocki, Mariusz J. - Ozegowska, Katarzyna - Jankowski, Maurycy - Ješeta, Michal - Pawelczyk, Leszek - Breborowicz, Andrzej - Rachon, Dominik - Skowronski, Mariusz T. - Bruska, Malgorzata - Zabel, Maciej - Nowicki, Michal - Kempisty, Bartosz
PY - 2019
TI - "Heart development and morphogenesis' is a novel pathway for human ovarian granulosa cell differentiation during long-term in vitro cultivation-a microarray approach
JF - Molecular Medicine Reports
VL - 19
IS - 3
SP - 1705-1715
EP - 1705-1715
PB - SPANDIDOS PUBL LTD
SN - 17912997
KW - human granulosa cells
KW - in vitro culture
KW - proliferation
KW - differentiation
KW - heart development
UR - https://www.ncbi.nlm.nih.gov/pubmed/30628715
L2 - https://www.ncbi.nlm.nih.gov/pubmed/30628715
N2 - Granulosa cells (GCs) have many functions in the endocrine system. Most notably, they produce progesterone following ovulation. However, it has recently been proven that GCs can change their properties when subjected to long-term culture. In the present study, GCs were collected from hyper-stimulated ovarian follicles during in vitro fertilization procedures. They were grown in vitro, in a long-term manner. RNA was collected following 1, 7, 15 and 30 days of culture. Expression microarrays were used for analysis, which allowed to identify groups of genes characteristic for particular cellular processes. In addition, reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was performed to validate the obtained results. Two ontological groups characteristic for processes associated with the development and morphogenesis of the heart were identified during the analyses: Heart development' and heart morphogenesis'. The results of the microarrays revealed that the highest change in expression was demonstrated by the lysyl Oxidase, oxytocin receptor, nexilin F-actin binding protein, and cysteine-rich protein 3 genes. The lowest change was exhibited by odd-skipped related transcription factor 1, plakophilin 2, transcription growth factor- receptor 1, and kinesin family member 3A. The direction of changes was confirmed by RT-qPCR results. In the present study, it was suggested that GCs may have the potential to differentiate towards other cell types under long-term in vitro culture conditions. Thus, genes belonging to the presented ontological groups can be considered as novel markers of proliferation and differentiation of GCs towards the heart muscle cells.
ER -
KRANC, Wieslawa, Maciej BRAZERT, Piotr CELICHOWSKI, Artur BRYJA, Mariusz J. NAWROCKI, Katarzyna OZEGOWSKA, Maurycy JANKOWSKI, Michal JEŠETA, Leszek PAWELCZYK, Andrzej BREBOROWICZ, Dominik RACHON, Mariusz T. SKOWRONSKI, Malgorzata BRUSKA, Maciej ZABEL, Michal NOWICKI and Bartosz KEMPISTY. ''Heart development and morphogenesis' is a novel pathway for human ovarian granulosa cell differentiation during long-term in vitro cultivation-a microarray approach. \textit{Molecular Medicine Reports}. ATHENS: SPANDIDOS PUBL LTD, 2019, vol.~19, No~3, p.~1705-1715. ISSN~1791-2997. Available from: https://dx.doi.org/10.3892/mmr.2019.9837.
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