Detailed Information on Publication Record
2019
"Heart development and morphogenesis' is a novel pathway for human ovarian granulosa cell differentiation during long-term in vitro cultivation-a microarray approach
KRANC, Wieslawa, Maciej BRAZERT, Piotr CELICHOWSKI, Artur BRYJA, Mariusz J. NAWROCKI et. al.Basic information
Original name
"Heart development and morphogenesis' is a novel pathway for human ovarian granulosa cell differentiation during long-term in vitro cultivation-a microarray approach
Authors
KRANC, Wieslawa (616 Poland), Maciej BRAZERT (616 Poland), Piotr CELICHOWSKI (616 Poland), Artur BRYJA (616 Poland), Mariusz J. NAWROCKI (616 Poland), Katarzyna OZEGOWSKA (616 Poland), Maurycy JANKOWSKI (616 Poland), Michal JEŠETA (203 Czech Republic, belonging to the institution), Leszek PAWELCZYK (616 Poland), Andrzej BREBOROWICZ (616 Poland), Dominik RACHON (616 Poland), Mariusz T. SKOWRONSKI (616 Poland), Malgorzata BRUSKA (616 Poland), Maciej ZABEL (616 Poland), Michal NOWICKI (616 Poland) and Bartosz KEMPISTY (616 Poland, guarantor)
Edition
Molecular Medicine Reports, ATHENS, SPANDIDOS PUBL LTD, 2019, 1791-2997
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
30214 Obstetrics and gynaecology
Country of publisher
Greece
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 2.100
RIV identification code
RIV/00216224:14110/19:00112995
Organization unit
Faculty of Medicine
UT WoS
000459710100031
Keywords in English
human granulosa cells; in vitro culture; proliferation; differentiation; heart development
Tags
International impact, Reviewed
Změněno: 14/4/2020 14:24, Mgr. Tereza Miškechová
Abstract
V originále
Granulosa cells (GCs) have many functions in the endocrine system. Most notably, they produce progesterone following ovulation. However, it has recently been proven that GCs can change their properties when subjected to long-term culture. In the present study, GCs were collected from hyper-stimulated ovarian follicles during in vitro fertilization procedures. They were grown in vitro, in a long-term manner. RNA was collected following 1, 7, 15 and 30 days of culture. Expression microarrays were used for analysis, which allowed to identify groups of genes characteristic for particular cellular processes. In addition, reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was performed to validate the obtained results. Two ontological groups characteristic for processes associated with the development and morphogenesis of the heart were identified during the analyses: Heart development' and heart morphogenesis'. The results of the microarrays revealed that the highest change in expression was demonstrated by the lysyl Oxidase, oxytocin receptor, nexilin F-actin binding protein, and cysteine-rich protein 3 genes. The lowest change was exhibited by odd-skipped related transcription factor 1, plakophilin 2, transcription growth factor- receptor 1, and kinesin family member 3A. The direction of changes was confirmed by RT-qPCR results. In the present study, it was suggested that GCs may have the potential to differentiate towards other cell types under long-term in vitro culture conditions. Thus, genes belonging to the presented ontological groups can be considered as novel markers of proliferation and differentiation of GCs towards the heart muscle cells.