The frequency and consequences of multipolar mitoses in
undifferentiated embryonic stem cells

J 2019

The frequency and consequences of multipolar mitoses in undifferentiated embryonic stem cells

POSPÍŠILOVÁ, Veronika, Milan EŠNER, Iveta ČERVENKOVÁ, Radek FEDR, Jean-Yvez TINEVEZ et. al.

Basic information

Original name

The frequency and consequences of multipolar mitoses in undifferentiated embryonic stem cells

Authors

POSPÍŠILOVÁ, Veronika (203 Czech Republic, belonging to the institution), Milan EŠNER (203 Czech Republic, belonging to the institution), Iveta ČERVENKOVÁ (703 Slovakia, belonging to the institution), Radek FEDR (203 Czech Republic), Jean-Yvez TINEVEZ (250 France), Aleš HAMPL (203 Czech Republic, belonging to the institution) and Martin ANGER (203 Czech Republic, guarantor, belonging to the institution)

Edition

Journal of applied biomedicine, České Budějovice, Faculty of Health and Social Care, University of South Bohemia, 2019, 1214-021X

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10601 Cell biology

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 1.700

RIV identification code

RIV/00216224:14110/19:00108159

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.32725/jab.2019.018

UT WoS

000510179600002

Keywords in English

Embryonic stem (ES) cells; Mitosis length; Multipolar division; Single-cell tracking; Spindle assembly checkpoint (SAC); Time-lapse microscopy

Abstract

V originále

Embryonic stem (ES) cells are pluripotent cells widely used in cell therapy and tissue engineering. However, the broader clinical applications of ES cells are limited by their genomic instability and karyotypic abnormalities. Thus, understanding the mechanisms underlying ES cell karyotypic abnormalities is critical to optimizing their dinical use. In this study, we focused on proliferating human and mouse ES cells undergoing multipolar divisions. Specifically, we analyzed the frequency and outcomes of such divisions using a combination of time-lapse microscopy and cell tracking. This revealed that cells resulting from multipolar divisions were not only viable, but they also frequently underwent subsequent cell divisions. Our novel data also showed that in human and mouse ES cells, multipolar spindles allowed more robust escape from chromosome segregation control mechanisms than bipolar spindles. Considering the frequency of multipolar divisions in proliferating ES cells, it is conceivable that cell division errors underlie ES cell karyotypic instability.

Links

EF16_013/0001775, research and development project

Name: Modernizace a podpora výzkumných aktivit národní infrastruktury pro biologické a medicínské zobrazování Czech-BioImaging

GA15-04844S, research and development project

Name: Osud aneuploidních buněk ve vyvíjejícím se embryu a populaci kmenových buněk

Investor: Czech Science Foundation

GA15-11707S, research and development project

Name: Centrosomální abnormality u lidských pluripotentních kmenových buněk.

Investor: Czech Science Foundation

LM2015043, research and development project

Name: Česká infrastruktura pro integrativní strukturní biologii (Acronym: CIISB)

Investor: Ministry of Education, Youth and Sports of the CR

LM2015062, research and development project

Name: Národní infrastruktura pro biologické a medicínské zobrazování

Investor: Ministry of Education, Youth and Sports of the CR

90043, large research infrastructures

Name: CIISB

90062, large research infrastructures

Name: Czech-BioImaging

Citovat

POSPÍŠILOVÁ, Veronika, Milan EŠNER, Iveta ČERVENKOVÁ, Radek FEDR, Jean-Yvez TINEVEZ, Aleš HAMPL and Martin ANGER. The frequency and consequences of multipolar mitoses in undifferentiated embryonic stem cells. Journal of applied biomedicine. České Budějovice: Faculty of Health and Social Care, University of South Bohemia, 2019, vol. 17, No 4, p. 209-217. ISSN 1214-021X. Available from: https://dx.doi.org/10.32725/jab.2019.018.

@article{1624157,
   author = {Pospíšilová, Veronika and Ešner, Milan and Červenková, Iveta and Fedr, Radek and Tinevez, JeanandYvez and Hampl, Aleš and Anger, Martin},
   article_location = {České Budějovice},
   article_number = {4},
   doi = {http://dx.doi.org/10.32725/jab.2019.018},
   keywords = {Embryonic stem (ES) cells; Mitosis length; Multipolar division; Single-cell tracking; Spindle assembly checkpoint (SAC); Time-lapse microscopy},
   language = {eng},
   issn = {1214-021X},
   journal = {Journal of applied biomedicine},
   title = {The frequency and consequences of multipolar mitoses in undifferentiated embryonic stem cells},
   url = {http://dx.doi.org/10.32725/jab.2019.018},
   volume = {17},
   year = {2019}
}

TY  - JOUR
ID  - 1624157
AU  - Pospíšilová, Veronika - Ešner, Milan - Červenková, Iveta - Fedr, Radek - Tinevez, Jean-Yvez - Hampl, Aleš - Anger, Martin
PY  - 2019
TI  - The frequency and consequences of multipolar mitoses in undifferentiated embryonic stem cells
JF  - Journal of applied biomedicine
VL  - 17
IS  - 4
SP  - 209-217
EP  - 209-217
PB  - Faculty of Health and Social Care, University of South Bohemia
SN  - 1214021X
KW  - Embryonic stem (ES) cells
KW  - Mitosis length
KW  - Multipolar division
KW  - Single-cell tracking
KW  - Spindle assembly checkpoint (SAC)
KW  - Time-lapse microscopy
UR  - http://dx.doi.org/10.32725/jab.2019.018
L2  - http://dx.doi.org/10.32725/jab.2019.018
N2  - Embryonic stem (ES) cells are pluripotent cells widely used in cell therapy and tissue engineering. However, the broader clinical applications of ES cells are limited by their genomic instability and karyotypic abnormalities. Thus, understanding the mechanisms underlying ES cell karyotypic abnormalities is critical to optimizing their dinical use. In this study, we focused on proliferating human and mouse ES cells undergoing multipolar divisions. Specifically, we analyzed the frequency and outcomes of such divisions using a combination of time-lapse microscopy and cell tracking. This revealed that cells resulting from multipolar divisions were not only viable, but they also frequently underwent subsequent cell divisions. Our novel data also showed that in human and mouse ES cells, multipolar spindles allowed more robust escape from chromosome segregation control mechanisms than bipolar spindles. Considering the frequency of multipolar divisions in proliferating ES cells, it is conceivable that cell division errors underlie ES cell karyotypic instability.
ER  -

POSPÍŠILOVÁ, Veronika, Milan EŠNER, Iveta ČERVENKOVÁ, Radek FEDR, Jean-Yvez TINEVEZ, Aleš HAMPL and Martin ANGER. The frequency and consequences of multipolar mitoses in undifferentiated embryonic stem cells. \textit{Journal of applied biomedicine}. České Budějovice: Faculty of Health and Social Care, University of South Bohemia, 2019, vol.~17, No~4, p.~209-217. ISSN~1214-021X. Available from: https://dx.doi.org/10.32725/jab.2019.018.

Detailed Information on Publication Record