Generation of human iPSCs from fetal prostate fibroblasts HPrF

J 2019

Generation of human iPSCs from fetal prostate fibroblasts HPrF

KAHOUNOVA, Zuzana, Eva SLABAKOVA, Lucia BINO, Jan REMSIK, Radek FEDR et. al.

Basic information

Original name

Generation of human iPSCs from fetal prostate fibroblasts HPrF

Authors

KAHOUNOVA, Zuzana (203 Czech Republic), Eva SLABAKOVA (203 Czech Republic), Lucia BINO (203 Czech Republic), Jan REMSIK (203 Czech Republic), Radek FEDR (203 Czech Republic), Jan BOUCHAL (203 Czech Republic), Radek VRTEL (203 Czech Republic), Lucie JUREČKOVÁ (203 Czech Republic, belonging to the institution), Volodymyr POROKH (804 Ukraine, belonging to the institution), Darja PARALOVA (203 Czech Republic), Aleš HAMPL (203 Czech Republic, belonging to the institution) and Karel SOUCEK (203 Czech Republic, guarantor)

Edition

Stem cell research, Amsterdam, Elsevier, 2019, 1873-5061

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10601 Cell biology

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 4.495

RIV identification code

RIV/00216224:14110/19:00108162

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1016/j.scr.2019.101405

UT WoS

000459831100019

Keywords in English

iPSCs; fetal prostate fibroblasts

Abstract

V originále

Human induced pluripotent stem cell line was generated from commercially available primary human prostate fibroblasts HPrF derived from a fetus, aged 18-24 weeks of gestation. The fibroblast cell line was reprogrammed with Yamanaka factors (OCT4, SOX2, c-MYC, KLF4) using CytoTune (TM)-iPS 2.0 Sendai Reprogramming Kit. Pluripotency of the derived transgene-free iPS cell line was confirmed both in vitro by detecting the expression of factors of pluripotency on a single-cell level, and in vivo using teratoma formation assay. This iPS cell line will be a useful tool for studying both normal prostate development and prostate cancer disease.

Links

GA15-11707S, research and development project

Name: Centrosomální abnormality u lidských pluripotentních kmenových buněk.

Investor: Czech Science Foundation

LM2015062, research and development project

Name: Národní infrastruktura pro biologické a medicínské zobrazování

Investor: Ministry of Education, Youth and Sports of the CR

MUNI/C/1418/2017, interní kód MU

Name: Extracelulární faktory ovlivňující metabolismus centrozomů v lidských pluripotentních kmenových buňkách

Investor: Masaryk University, Rector's Program

Citovat

KAHOUNOVA, Zuzana, Eva SLABAKOVA, Lucia BINO, Jan REMSIK, Radek FEDR, Jan BOUCHAL, Radek VRTEL, Lucie JUREČKOVÁ, Volodymyr POROKH, Darja PARALOVA, Aleš HAMPL and Karel SOUCEK. Generation of human iPSCs from fetal prostate fibroblasts HPrF. Stem cell research. Amsterdam: Elsevier, 2019, vol. 35, MAR 2019, p. 1-5. ISSN 1873-5061. Available from: https://dx.doi.org/10.1016/j.scr.2019.101405.

@article{1624637,
   author = {Kahounova, Zuzana and Slabakova, Eva and Bino, Lucia and Remsik, Jan and Fedr, Radek and Bouchal, Jan and Vrtel, Radek and Jurečková, Lucie and Porokh, Volodymyr and Paralova, Darja and Hampl, Aleš and Soucek, Karel},
   article_location = {Amsterdam},
   article_number = {MAR 2019},
   doi = {http://dx.doi.org/10.1016/j.scr.2019.101405},
   keywords = {iPSCs; fetal prostate fibroblasts},
   language = {eng},
   issn = {1873-5061},
   journal = {Stem cell research},
   title = {Generation of human iPSCs from fetal prostate fibroblasts HPrF},
   url = {https://www.sciencedirect.com/science/article/pii/S1873506119300352},
   volume = {35},
   year = {2019}
}

TY  - JOUR
ID  - 1624637
AU  - Kahounova, Zuzana - Slabakova, Eva - Bino, Lucia - Remsik, Jan - Fedr, Radek - Bouchal, Jan - Vrtel, Radek - Jurečková, Lucie - Porokh, Volodymyr - Paralova, Darja - Hampl, Aleš - Soucek, Karel
PY  - 2019
TI  - Generation of human iPSCs from fetal prostate fibroblasts HPrF
JF  - Stem cell research
VL  - 35
IS  - MAR 2019
SP  - 1-5
EP  - 1-5
PB  - Elsevier
SN  - 18735061
KW  - iPSCs
KW  - fetal prostate fibroblasts
UR  - https://www.sciencedirect.com/science/article/pii/S1873506119300352
L2  - https://www.sciencedirect.com/science/article/pii/S1873506119300352
N2  - Human induced pluripotent stem cell line was generated from commercially available primary human prostate fibroblasts HPrF derived from a fetus, aged 18-24 weeks of gestation. The fibroblast cell line was reprogrammed with Yamanaka factors (OCT4, SOX2, c-MYC, KLF4) using CytoTune (TM)-iPS 2.0 Sendai Reprogramming Kit. Pluripotency of the derived transgene-free iPS cell line was confirmed both in vitro by detecting the expression of factors of pluripotency on a single-cell level, and in vivo using teratoma formation assay. This iPS cell line will be a useful tool for studying both normal prostate development and prostate cancer disease.
ER  -

KAHOUNOVA, Zuzana, Eva SLABAKOVA, Lucia BINO, Jan REMSIK, Radek FEDR, Jan BOUCHAL, Radek VRTEL, Lucie JUREČKOVÁ, Volodymyr POROKH, Darja PARALOVA, Aleš HAMPL and Karel SOUCEK. Generation of human iPSCs from fetal prostate fibroblasts HPrF. \textit{Stem cell research}. Amsterdam: Elsevier, 2019, vol.~35, MAR 2019, p.~1-5. ISSN~1873-5061. Available from: https://dx.doi.org/10.1016/j.scr.2019.101405.

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