TRANSCRIPTOMIC PROFILE OF CELL CYCLE PROGRESSION GENES IN HUMAN OVARIAN GRANULOSA CELLS

BRAZERT, M., D. IZYCKI, W. KRANC, B. BOROWIEC, M. POPIS, P. CELICHOWSKI, K. OZEGOWSKA, M. JANKOWSKI, Michal JEŠETA, L. PAWELCZYK, A. BREBOROWICZ, D. RACHON, M. NOWICKI and B. KEMPISTY. TRANSCRIPTOMIC PROFILE OF CELL CYCLE PROGRESSION GENES IN HUMAN OVARIAN GRANULOSA CELLS. Journal of biological regulators and homeostatic agents. Silva Marina: Biolife SAS, 2019, vol. 33, No 1, p. 39-51. ISSN 0393-974X.

Basic information

Original name

TRANSCRIPTOMIC PROFILE OF CELL CYCLE PROGRESSION GENES IN HUMAN OVARIAN GRANULOSA CELLS

Authors

BRAZERT, M. (616 Poland), D. IZYCKI (616 Poland), W. KRANC (616 Poland), B. BOROWIEC (616 Poland), M. POPIS (616 Poland), P. CELICHOWSKI (616 Poland), K. OZEGOWSKA (616 Poland), M. JANKOWSKI (616 Poland), Michal JEŠETA (203 Czech Republic, belonging to the institution), L. PAWELCZYK (616 Poland), A. BREBOROWICZ (616 Poland), D. RACHON (616 Poland), M. NOWICKI (616 Poland) and B. KEMPISTY (616 Poland, guarantor)

Edition

Journal of biological regulators and homeostatic agents, Silva Marina, Biolife SAS, 2019, 0393-974X

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Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30214 Obstetrics and gynaecology

Country of publisher

Italy

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 1.506

RIV identification code

RIV/00216224:14110/19:00113002

Organization unit

Faculty of Medicine

UT WoS

000464697500005

Keywords in English

human ovarian granulosa cells; cell cycle; progression; in vitro

Tags

14110411, rivok

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Abstract

V originále

The ovarian granulosa cells (GCs) that form the structure of follicle undergo substantial modification during the various stages of human folliculogenesis. These modifications include morphological changes, accompanied by differential expression of genes, encoding proteins which are mainly involved in cell growth, proliferation and differentiation. Recent data bring a new insight into the aspects of GCs' stem-like specificity and plasticity, enabling their prolonged proliferation and differentiation into other cell types. This manuscript focuses attention on emerging alterations during GC cell cycle - a series of biochemical and biophysical changes within the cell. Human GCs were collected from follicles of women set to undergo intracytoplasmic sperm injection procedure, as a part of remnant follicular fluid. The cells were primarily cultured for 30 days. Throughout this time, we observed the prominent change in cell morphology from epithelial-like to fibroblast-like, suggesting differentiation to other cell types. Additionally, at days 1, 7, 15 and 30, the RNA was isolated for molecular assays. Using Affymetrie (R) Human Genome U219 Array, we found 2579 human transcripts that were differentially expressed in GCs. From these genes, we extracted 582 Gene Ontology Biological Process (GO BP) Terms and 45 KEGG pathways, among which we investigated transcripts belonging to four GO BPs associated ith cell proliferation: "cell cycle phase transition", "Gl/S phase transition", G2/M phase transition" and "cell cycle checkpoint". Microarray results were validated by RT-qPCR. Increased expression of all the genes studied indicated that increase in GC proliferation during long-term in vitro culture is orchestrated by the up-regulation of genes related to cell cycle control. Furthermore, observed changes in cell morphology may be regulated by a presented set of genes, leading to the induction of pathways specific for sternness plasticity and transdifferentiation in vitro.