J 2019

INSIGHT INTO "NUCLEAR-CYTOPLASMIC SHUTTLING" AS A DEVELOPMENTAL AND DIFFERENTIATIONAL CAPABILITY OF CELLS IN PRIMARY CULTURE MODELS

KULUS, M., M. BRAZERT, M. POPIS, B. BOROWIEC, D. BUKOWSKA et. al.

Basic information

Original name

INSIGHT INTO "NUCLEAR-CYTOPLASMIC SHUTTLING" AS A DEVELOPMENTAL AND DIFFERENTIATIONAL CAPABILITY OF CELLS IN PRIMARY CULTURE MODELS

Authors

KULUS, M. (616 Poland), M. BRAZERT (616 Poland), M. POPIS (616 Poland), B. BOROWIEC (616 Poland), D. BUKOWSKA (616 Poland), Michal JEŠETA (203 Czech Republic, belonging to the institution), H. PIOTROWSKA-KEMPISTY (616 Poland), M. NOWICKI (616 Poland), B. KEMPISTY (616 Poland, guarantor) and P. ANTOSIK (616 Poland)

Edition

Journal of biological regulators and homeostatic agents, Silva Marina, Biolife SAS, 2019, 0393-974X

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10601 Cell biology

Country of publisher

Italy

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 1.506

RIV identification code

RIV/00216224:14110/19:00113004

Organization unit

Faculty of Medicine

UT WoS

000464697500019

Keywords in English

primary cell model; in vitro growth and development; nuclear-cytoplasmic shuttling

Tags

Tags

International impact, Reviewed
Změněno: 14/4/2020 14:25, Mgr. Tereza Miškechová

Abstract

V originále

Shuttling proteins are molecules that can facilitate transport through the nuclear envelope. A very large number of proteins are involved in this process that includes nuclear pore buildup, signal, receptor and enzyme proteins. There are many examples of proteins whose biological activity depends on nucleocytoplasmic transport. Very often they are largely responsible for the proper occurrence of cell division, maturation, development and differentiation. Thanks to the well mastered methods of in vitro cell culture, it is possible to trace the levels of protein expression and their distribution in cells. Advanced molecular techniques allow for precise determination of their displacement in time. Several studies are still being carried out, using primary cultures, to identify the factors that determine the maturation, development and differentiation of cells. In understanding of the detailed mechanisms controlling cell life, the key is not the level of expression of a specific protein, but its distribution in individual cellular compartments.