Detailed Information on Publication Record
2020
Rapid Multiplex Real-Time PCR Method for the Detection and Quantification of Selected Cariogenic and Periodontal Bacteria
LOCHMAN, Jan, Martina ZAPLETALOVÁ, Hana POSKEROVÁ, Lydie IZAKOVIČOVÁ HOLLÁ, Petra BOŘILOVÁ LINHARTOVÁ et. al.Basic information
Original name
Rapid Multiplex Real-Time PCR Method for the Detection and Quantification of Selected Cariogenic and Periodontal Bacteria
Authors
LOCHMAN, Jan (203 Czech Republic, belonging to the institution), Martina ZAPLETALOVÁ (203 Czech Republic, belonging to the institution), Hana POSKEROVÁ (203 Czech Republic, belonging to the institution), Lydie IZAKOVIČOVÁ HOLLÁ (203 Czech Republic, belonging to the institution) and Petra BOŘILOVÁ LINHARTOVÁ (203 Czech Republic, guarantor, belonging to the institution)
Edition
Diagnostics, Basel, MDPI, 2020, 2075-4418
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
30218 General and internal medicine
Country of publisher
Switzerland
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 3.706
RIV identification code
RIV/00216224:14110/20:00118596
Organization unit
Faculty of Medicine
UT WoS
000513129500027
Keywords in English
dental caries; periodontitis; dysbiosis; oral microbiome; risk factors determination
Tags
International impact, Reviewed
Změněno: 15/9/2020 11:08, Mgr. Tereza Miškechová
Abstract
V originále
Dental caries and periodontal diseases are associated with a shift from symbiotic microbiota to dysbiosis. The aim of our study was to develop a rapid, sensitive, and economical method for the identification and quantification of selected cariogenic and periodontal oral bacteria. Original protocols were designed for three real-time multiplex PCR assays to detect and quantify the ratio of 10 bacterial species associated with dental caries ("cariogenic" complex) or periodontal diseases (red complex, orange complex, and Aggregatibacter actinomycetemcomitans). A total number of 60 samples from 30 children aged 2-6 years with severe early childhood caries and gingivitis were tested. In multiplex assays, the quantification of total bacterial (TB) content for cariogenic bacteria and red complex to eliminate differences in quantities caused by specimen collection was included. The mean counts for the TB load and that of ten evaluated specimens corresponded to previously published results. We found a significant difference between the microbial compositions obtained from the area of control and the affected teeth (p < 0.05). Based on this comprehensive microbiological examination, the risk of dental caries or periodontal inflammation may be determined. The test could also be used as a tool for behavioral intervention and thus prevention of the above-mentioned diseases.
Links
| NV17-30439A, research and development project |
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