Rapid Multiplex Real-Time PCR Method for the Detection and
Quantification of Selected Cariogenic and Periodontal Bacteria

J 2020

Rapid Multiplex Real-Time PCR Method for the Detection and Quantification of Selected Cariogenic and Periodontal Bacteria

LOCHMAN, Jan, Martina ZAPLETALOVÁ, Hana POSKEROVÁ, Lydie IZAKOVIČOVÁ HOLLÁ, Petra BOŘILOVÁ LINHARTOVÁ et. al.

Basic information

Original name

Rapid Multiplex Real-Time PCR Method for the Detection and Quantification of Selected Cariogenic and Periodontal Bacteria

Authors

LOCHMAN, Jan (203 Czech Republic, belonging to the institution), Martina ZAPLETALOVÁ (203 Czech Republic, belonging to the institution), Hana POSKEROVÁ (203 Czech Republic, belonging to the institution), Lydie IZAKOVIČOVÁ HOLLÁ (203 Czech Republic, belonging to the institution) and Petra BOŘILOVÁ LINHARTOVÁ (203 Czech Republic, guarantor, belonging to the institution)

Edition

Diagnostics, Basel, MDPI, 2020, 2075-4418

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30218 General and internal medicine

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 3.706

RIV identification code

RIV/00216224:14110/20:00118596

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.3390/diagnostics10010008

UT WoS

000513129500027

Keywords in English

dental caries; periodontitis; dysbiosis; oral microbiome; risk factors determination

Abstract

V originále

Dental caries and periodontal diseases are associated with a shift from symbiotic microbiota to dysbiosis. The aim of our study was to develop a rapid, sensitive, and economical method for the identification and quantification of selected cariogenic and periodontal oral bacteria. Original protocols were designed for three real-time multiplex PCR assays to detect and quantify the ratio of 10 bacterial species associated with dental caries ("cariogenic" complex) or periodontal diseases (red complex, orange complex, and Aggregatibacter actinomycetemcomitans). A total number of 60 samples from 30 children aged 2-6 years with severe early childhood caries and gingivitis were tested. In multiplex assays, the quantification of total bacterial (TB) content for cariogenic bacteria and red complex to eliminate differences in quantities caused by specimen collection was included. The mean counts for the TB load and that of ten evaluated specimens corresponded to previously published results. We found a significant difference between the microbial compositions obtained from the area of control and the affected teeth (p < 0.05). Based on this comprehensive microbiological examination, the risk of dental caries or periodontal inflammation may be determined. The test could also be used as a tool for behavioral intervention and thus prevention of the above-mentioned diseases.

Links

NV17-30439A, research and development project

Name: Moderní biotechnologické a behaviorální přístupy ve výzkumu zubního kazu a strategie jeho prevence

Citovat

LOCHMAN, Jan, Martina ZAPLETALOVÁ, Hana POSKEROVÁ, Lydie IZAKOVIČOVÁ HOLLÁ and Petra BOŘILOVÁ LINHARTOVÁ. Rapid Multiplex Real-Time PCR Method for the Detection and Quantification of Selected Cariogenic and Periodontal Bacteria. Diagnostics. Basel: MDPI, 2020, vol. 10, No 1, p. 1-14. ISSN 2075-4418. Available from: https://dx.doi.org/10.3390/diagnostics10010008.

@article{1630876,
   author = {Lochman, Jan and Zapletalová, Martina and Poskerová, Hana and Izakovičová Hollá, Lydie and Bořilová Linhartová, Petra},
   article_location = {Basel},
   article_number = {1},
   doi = {http://dx.doi.org/10.3390/diagnostics10010008},
   keywords = {dental caries; periodontitis; dysbiosis; oral microbiome; risk factors determination},
   language = {eng},
   issn = {2075-4418},
   journal = {Diagnostics},
   title = {Rapid Multiplex Real-Time PCR Method for the Detection and Quantification of Selected Cariogenic and Periodontal Bacteria},
   url = {https://www.mdpi.com/2075-4418/10/1/8},
   volume = {10},
   year = {2020}
}

TY  - JOUR
ID  - 1630876
AU  - Lochman, Jan - Zapletalová, Martina - Poskerová, Hana - Izakovičová Hollá, Lydie - Bořilová Linhartová, Petra
PY  - 2020
TI  - Rapid Multiplex Real-Time PCR Method for the Detection and Quantification of Selected Cariogenic and Periodontal Bacteria
JF  - Diagnostics
VL  - 10
IS  - 1
SP  - 1-14
EP  - 1-14
PB  - MDPI
SN  - 20754418
KW  - dental caries
KW  - periodontitis
KW  - dysbiosis
KW  - oral microbiome
KW  - risk factors determination
UR  - https://www.mdpi.com/2075-4418/10/1/8
L2  - https://www.mdpi.com/2075-4418/10/1/8
N2  - Dental caries and periodontal diseases are associated with a shift from symbiotic microbiota to dysbiosis. The aim of our study was to develop a rapid, sensitive, and economical method for the identification and quantification of selected cariogenic and periodontal oral bacteria. Original protocols were designed for three real-time multiplex PCR assays to detect and quantify the ratio of 10 bacterial species associated with dental caries ("cariogenic" complex) or periodontal diseases (red complex, orange complex, and Aggregatibacter actinomycetemcomitans). A total number of 60 samples from 30 children aged 2-6 years with severe early childhood caries and gingivitis were tested. In multiplex assays, the quantification of total bacterial (TB) content for cariogenic bacteria and red complex to eliminate differences in quantities caused by specimen collection was included. The mean counts for the TB load and that of ten evaluated specimens corresponded to previously published results. We found a significant difference between the microbial compositions obtained from the area of control and the affected teeth (p < 0.05). Based on this comprehensive microbiological examination, the risk of dental caries or periodontal inflammation may be determined. The test could also be used as a tool for behavioral intervention and thus prevention of the above-mentioned diseases.
ER  -

LOCHMAN, Jan, Martina ZAPLETALOVÁ, Hana POSKEROVÁ, Lydie IZAKOVIČOVÁ HOLLÁ and Petra BOŘILOVÁ LINHARTOVÁ. Rapid Multiplex Real-Time PCR Method for the Detection and Quantification of Selected Cariogenic and Periodontal Bacteria. \textit{Diagnostics}. Basel: MDPI, 2020, vol.~10, No~1, p.~1-14. ISSN~2075-4418. Available from: https://dx.doi.org/10.3390/diagnostics10010008.

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