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@article{1635179,
author = {Fischer, C. and Metsger, Maria and Bauch, S. and Vidal, R. and Bottcher, M. and Grote, P. and Kliem, M. and Sauer, S.},
article_location = {WASHINGTON},
article_number = {581},
doi = {http://dx.doi.org/10.1126/scisignal.aao5820},
keywords = {TOLL-LIKE RECEPTORS; RNA-SEQ; GENE-EXPRESSION; R-PACKAGE; REVEALS; MACROPHAGES; ACTIVATION; MECHANISMS; REGULATOR; SPECTRUM},
language = {eng},
issn = {1945-0877},
journal = {SCIENCE SIGNALING},
title = {Signals trigger state-specific transcriptional programs to support diversity and homeostasis in immune cells},
url = {https://stke.sciencemag.org/content/12/581/eaao5820},
volume = {12},
year = {2019}
}
TY - JOUR
ID - 1635179
AU - Fischer, C. - Metsger, Maria - Bauch, S. - Vidal, R. - Bottcher, M. - Grote, P. - Kliem, M. - Sauer, S.
PY - 2019
TI - Signals trigger state-specific transcriptional programs to support diversity and homeostasis in immune cells
JF - SCIENCE SIGNALING
VL - 12
IS - 581
SP - 1-16
EP - 1-16
PB - AMER ASSOC ADVANCEMENT SCIENCE
SN - 19450877
KW - TOLL-LIKE RECEPTORS
KW - RNA-SEQ
KW - GENE-EXPRESSION
KW - R-PACKAGE
KW - REVEALS
KW - MACROPHAGES
KW - ACTIVATION
KW - MECHANISMS
KW - REGULATOR
KW - SPECTRUM
UR - https://stke.sciencemag.org/content/12/581/eaao5820
L2 - https://stke.sciencemag.org/content/12/581/eaao5820
N2 - Macrophages play key roles in the immune systems of humans and other mammals. Here, we performed single-cell analyses of the mRNAs and proteins of human macrophages to compare their responses to the signaling molecules lipopolysaccharide (LPS), a component of Gram-negative bacteria, and palmitate (PAL), a free fatty acid. We found that, although both molecules signal through the cell surface protein Toll-like receptor 4 (TLR4), they stimulated the expression of different genes, resulting in specific pro- and anti-inflammatory cellular states for each signal. The effects of the glucocorticoid receptor, which antagonizes LPS signaling, and cyclic AMP-dependent transcription factor 3, which inhibits PAL-induced inflammation, on inflammatory response seemed largely determined by digital on-off events. Furthermore, the quantification of transcriptional variance and signaling entropy enabled the identification of cell state-specific deregulated molecular pathways. These data suggest that the preservation of signaling in distinct cells might confer diversity on macrophage populations essential to maintaining major cellular functions.
ER -
FISCHER, C., Maria METSGER, S. BAUCH, R. VIDAL, M. BOTTCHER, P. GROTE, M. KLIEM a S. SAUER. Signals trigger state-specific transcriptional programs to support diversity and homeostasis in immune cells. \textit{SCIENCE SIGNALING}. WASHINGTON: AMER ASSOC ADVANCEMENT SCIENCE, 2019, roč.~12, č.~581, s.~1-16. ISSN~1945-0877. Dostupné z: https://dx.doi.org/10.1126/scisignal.aao5820.
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