| WEBER, J., J. DELA ROSA, C.S. GROVE, M. SCHICK, L. RAD, O. BARANOVT, A. STRONG, A. PFAUS, M.J. FRIEDRICH, T. ENGLEITNER, R. LERSCH, R. OLLINGER, M. GRAU, I.G. MENENDEZ, M. MARTELLA, U. KOHLHOFER, R. BANERJEE, M.A. TURCHANINOVA, A. SCHERGER, G.J. HOFFMAN, J. HESS, L.B. KUH, T. AMMON, J. KIM, G. SCHNEIDER, K. UNGER, U. ZIMBER-STROBL, M. HEIKENWALDER, M. SCHMIDT-SUPPRIAN, F.T. YANG, D. SAUR, P.T. LIU, K. STEIGER, Dmitriy CHUDAKOV, G. LENZ, L. QUINTANILLA-MARTINEZ, U. KELLER, G.S. VASSILIOU, J. CADINANOS, A. BRADLEY and R. RAD. PiggyBac transposon tools for recessive screening identify B-cell lymphoma drivers in mice. Nature Communications. London: Nature Publishing Group, 2019, vol. 10, MAR, p. 1-16. ISSN 2041-1723. Available from: https://dx.doi.org/10.1038/s41467-019-09180-3. |
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@article{1635280,
author = {Weber, J. and dela Rosa, J. and Grove, C.S. and Schick, M. and Rad, L. and Baranovt, O. and Strong, A. and Pfaus, A. and Friedrich, M.J. and Engleitner, T. and Lersch, R. and Ollinger, R. and Grau, M. and Menendez, I.G. and Martella, M. and Kohlhofer, U. and Banerjee, R. and Turchaninova, M.A. and Scherger, A. and Hoffman, G.J. and Hess, J. and Kuh, L.B. and Ammon, T. and Kim, J. and Schneider, G. and Unger, K. and ZimberandStrobl, U. and Heikenwalder, M. and SchmidtandSupprian, M. and Yang, F.T. and Saur, D. and Liu, P.T. and Steiger, K. and Chudakov, Dmitriy and Lenz, G. and QuintanillaandMartinez, L. and Keller, U. and Vassiliou, G.S. and Cadinanos, J. and Bradley, A. and Rad, R.},
article_location = {London},
article_number = {MAR},
doi = {http://dx.doi.org/10.1038/s41467-019-09180-3},
keywords = {CANCER GENE DISCOVERY; SLEEPING-BEAUTY; CHROMOSOMAL TRANSPOSITION; INSERTIONAL MUTAGENESIS; THERAPEUTIC TARGETS; CODING GENOME; MOUSE MODEL; PATHOGENESIS; ACTIVATION; MUTATION},
language = {eng},
issn = {2041-1723},
journal = {Nature Communications},
title = {PiggyBac transposon tools for recessive screening identify B-cell lymphoma drivers in mice},
url = {https://www.nature.com/articles/s41467-019-09180-3},
volume = {10},
year = {2019}
}
TY - JOUR
ID - 1635280
AU - Weber, J. - dela Rosa, J. - Grove, C.S. - Schick, M. - Rad, L. - Baranovt, O. - Strong, A. - Pfaus, A. - Friedrich, M.J. - Engleitner, T. - Lersch, R. - Ollinger, R. - Grau, M. - Menendez, I.G. - Martella, M. - Kohlhofer, U. - Banerjee, R. - Turchaninova, M.A. - Scherger, A. - Hoffman, G.J. - Hess, J. - Kuh, L.B. - Ammon, T. - Kim, J. - Schneider, G. - Unger, K. - Zimber-Strobl, U. - Heikenwalder, M. - Schmidt-Supprian, M. - Yang, F.T. - Saur, D. - Liu, P.T. - Steiger, K. - Chudakov, Dmitriy - Lenz, G. - Quintanilla-Martinez, L. - Keller, U. - Vassiliou, G.S. - Cadinanos, J. - Bradley, A. - Rad, R.
PY - 2019
TI - PiggyBac transposon tools for recessive screening identify B-cell lymphoma drivers in mice
JF - Nature Communications
VL - 10
IS - MAR
SP - 1-16
EP - 1-16
PB - Nature Publishing Group
SN - 20411723
KW - CANCER GENE DISCOVERY
KW - SLEEPING-BEAUTY
KW - CHROMOSOMAL TRANSPOSITION
KW - INSERTIONAL MUTAGENESIS
KW - THERAPEUTIC TARGETS
KW - CODING GENOME
KW - MOUSE MODEL
KW - PATHOGENESIS
KW - ACTIVATION
KW - MUTATION
UR - https://www.nature.com/articles/s41467-019-09180-3
L2 - https://www.nature.com/articles/s41467-019-09180-3
N2 - B-cell lymphoma (BCL) is the most common hematologic malignancy. While sequencing studies gave insights into BCL genetics, identification of non-mutated cancer genes remains challenging. Here, we describe PiggyBac transposon tools and mouse models for recessive screening and show their application to study clonal B-cell lymphomagenesis. In a genome-wide screen, we discover BCL genes related to diverse molecular processes, including signaling, transcriptional regulation, chromatin regulation, or RNA metabolism. Cross-species analyses show the efficiency of the screen to pinpoint human cancer drivers altered by non-genetic mechanisms, including clinically relevant genes dysregulated epigenetically, transcriptionally, or post-transcriptionally in human BCL. We also describe a CRISPR/Cas9-based in vivo platform for BCL functional genomics, and validate discovered genes, such as Rfx7, a transcription factor, and Phip, a chromatin regulator, which suppress lymphomagenesis in mice. Our study gives comprehensive insights into the molecular landscapes of BCL and underlines the power of genome-scale screening to inform biology.
ER -
WEBER, J., J. DELA ROSA, C.S. GROVE, M. SCHICK, L. RAD, O. BARANOVT, A. STRONG, A. PFAUS, M.J. FRIEDRICH, T. ENGLEITNER, R. LERSCH, R. OLLINGER, M. GRAU, I.G. MENENDEZ, M. MARTELLA, U. KOHLHOFER, R. BANERJEE, M.A. TURCHANINOVA, A. SCHERGER, G.J. HOFFMAN, J. HESS, L.B. KUH, T. AMMON, J. KIM, G. SCHNEIDER, K. UNGER, U. ZIMBER-STROBL, M. HEIKENWALDER, M. SCHMIDT-SUPPRIAN, F.T. YANG, D. SAUR, P.T. LIU, K. STEIGER, Dmitriy CHUDAKOV, G. LENZ, L. QUINTANILLA-MARTINEZ, U. KELLER, G.S. VASSILIOU, J. CADINANOS, A. BRADLEY and R. RAD. PiggyBac transposon tools for recessive screening identify B-cell lymphoma drivers in mice. \textit{Nature Communications}. London: Nature Publishing Group, 2019, vol.~10, MAR, p.~1-16. ISSN~2041-1723. Available from: https://dx.doi.org/10.1038/s41467-019-09180-3.
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