Detailed Information on Publication Record
2019
Molecular characterization of Chlamydomonas reinhardtii telomeres and telomerase mutants
EBERHARD, S., Soňa VALUCHOVÁ, J. RAVAT, Jaroslav FULNEČEK, P. JOLIVET et. al.Basic information
Original name
Molecular characterization of Chlamydomonas reinhardtii telomeres and telomerase mutants
Authors
EBERHARD, S., Soňa VALUCHOVÁ (703 Slovakia, belonging to the institution), J. RAVAT, Jaroslav FULNEČEK (203 Czech Republic, belonging to the institution), P. JOLIVET, S. BUJALDON, S.D. LEMAIRE, F.A. WOLLMAN, M.T. TEIXEIRA, Karel ŘÍHA (203 Czech Republic, guarantor, belonging to the institution) and Z. XU
Edition
LIFE SCIENCE ALLIANCE, COLD SPRING HARBOR, LIFE SCIENCE ALLIANCE LLC, 2019, 2575-1077
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10611 Plant sciences, botany
Country of publisher
United States of America
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 2.622
RIV identification code
RIV/00216224:14740/19:00113265
Organization unit
Central European Institute of Technology
UT WoS
000473222200015
Keywords in English
GENOME-WIDE SCREEN; LENGTH REGULATION; CATALYTIC SUBUNIT; ARABIDOPSIS; YEAST; SEQUENCE; ELONGATION; MECHANISM; EVOLUTION; REPEATS
Tags
Tags
International impact, Reviewed
Změněno: 16/3/2020 11:12, Mgr. Pavla Foltynová, Ph.D.
Abstract
V originále
Telomeres are repeated sequences found at the end of the linear chromosomes of most eukaryotes and are required for chromosome integrity. Expression of the reverse-transcriptase telomerase allows for extension of telomeric repeats to counteract natural telomere shortening. Although Chlamydomonas reinhardtii, a photosynthetic unicellular green alga, is widely used as a model organism in photosynthesis and flagella research, and for biotechnological applications, the biology of its telomeres has not been investigated in depth. Here, we show that the C. reinhardtii (TTTTAGGG)(n) telomeric repeats are mostly nondegenerate and that the telomeres form a protective structure, with a subset ending with a 3' overhang and another subset presenting a blunt end. Although telomere size and length distributions are stable under various standard growth conditions, they vary substantially between 12 genetically close reference strains. Finally, we identify CrTERT, the gene encoding the catalytic subunit of telomerase and show that telomeres shorten progressively in mutants of this gene. Telomerase mutants eventually enter replicative senescence, demonstrating that telomerase is required for long-term maintenance of telomeres in C. reinhardtii.
Links
EF15_003/0000479, research and development project |
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