A role for the auxin precursor anthranilic acid in root
gravitropism via regulation of PIN-FORMED protein polarity and
relocalisation in Arabidopsis

J 2019

A role for the auxin precursor anthranilic acid in root gravitropism via regulation of PIN-FORMED protein polarity and relocalisation in Arabidopsis

DOYLE, S.M., A. RIGAL, P. GRONES, M. KARADY, D.K. BARANGE et. al.

Basic information

Original name

A role for the auxin precursor anthranilic acid in root gravitropism via regulation of PIN-FORMED protein polarity and relocalisation in Arabidopsis

Authors

DOYLE, S.M., A. RIGAL, P. GRONES, M. KARADY, D.K. BARANGE, M. MAJDA, B. PARIZKOVA, M. KARAMPELIAS, Marta ZWIEWKA (616 Poland, guarantor, belonging to the institution), A. PENCIK, F. ALMQVIST, K. LJUNG, O. NOVAK and S. ROBERT

Edition

New Phytologist, HOBOKEN, Blackwell Science, 2019, 0028-646X

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10611 Plant sciences, botany

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 8.512

RIV identification code

RIV/00216224:14740/19:00108188

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1111/nph.15877

UT WoS

000475918000032

Keywords in English

anthranilic acid (AA); Arabidopsis thaliana; auxin transport; PIN polarity; PIN-FORMED proteins; root gravitropism

Abstract

V originále

distribution of auxin within plant tissues is of great importance for developmental plasticity, including root gravitropic growth. Auxin flow is directed by the subcellular polar distribution and dynamic relocalisation of auxin transporters such as the PIN-FORMED (PIN) efflux carriers, which can be influenced by the main natural plant auxin indole-3-acetic acid (IAA). Anthranilic acid (AA) is an important early precursor of IAA and previously published studies with AA analogues have suggested that AA may also regulate PIN localisation. Using Arabidopsis thaliana as a model species, we studied an AA-deficient mutant displaying agravitropic root growth, treated seedlings with AA and AA analogues and transformed lines to over-produce AA while inhibiting its conversion to downstream IAA precursors. We showed that AA rescues root gravitropic growth in the AA-deficient mutant at concentrations that do not rescue IAA levels. Overproduction of AA affects root gravitropism without affecting IAA levels. Treatments with, or deficiency in, AA result in defects in PIN polarity and gravistimulus-induced PIN relocalisation in root cells. Our results revealed a previously unknown role for AA in the regulation of PIN subcellular localisation and dynamics involved in root gravitropism, which is independent of its better known role in IAA biosynthesis.

Links

GA13-40637S, research and development project

Name: Genetické studie k identifikaci molekulárních mechanizmů buněčné polarity a auxinového transportu v rostlinách

Investor: Czech Science Foundation

90062, large research infrastructures

Name: Czech-BioImaging

Citovat

DOYLE, S.M., A. RIGAL, P. GRONES, M. KARADY, D.K. BARANGE, M. MAJDA, B. PARIZKOVA, M. KARAMPELIAS, Marta ZWIEWKA, A. PENCIK, F. ALMQVIST, K. LJUNG, O. NOVAK and S. ROBERT. A role for the auxin precursor anthranilic acid in root gravitropism via regulation of PIN-FORMED protein polarity and relocalisation in Arabidopsis. New Phytologist. HOBOKEN: Blackwell Science, 2019, vol. 223, No 3, p. 1420-1432. ISSN 0028-646X. Available from: https://dx.doi.org/10.1111/nph.15877.

@article{1636883,
   author = {Doyle, S.M. and Rigal, A. and Grones, P. and Karady, M. and Barange, D.K. and Majda, M. and Parizkova, B. and Karampelias, M. and Zwiewka, Marta and Pencik, A. and Almqvist, F. and Ljung, K. and Novak, O. and Robert, S.},
   article_location = {HOBOKEN},
   article_number = {3},
   doi = {http://dx.doi.org/10.1111/nph.15877},
   keywords = {anthranilic acid (AA); Arabidopsis thaliana; auxin transport; PIN polarity; PIN-FORMED proteins; root gravitropism},
   language = {eng},
   issn = {0028-646X},
   journal = {New Phytologist},
   title = {A role for the auxin precursor anthranilic acid in root gravitropism via regulation of PIN-FORMED protein polarity and relocalisation in Arabidopsis},
   url = {https://nph.onlinelibrary.wiley.com/doi/full/10.1111/nph.15877},
   volume = {223},
   year = {2019}
}

TY  - JOUR
ID  - 1636883
AU  - Doyle, S.M. - Rigal, A. - Grones, P. - Karady, M. - Barange, D.K. - Majda, M. - Parizkova, B. - Karampelias, M. - Zwiewka, Marta - Pencik, A. - Almqvist, F. - Ljung, K. - Novak, O. - Robert, S.
PY  - 2019
TI  - A role for the auxin precursor anthranilic acid in root gravitropism via regulation of PIN-FORMED protein polarity and relocalisation in Arabidopsis
JF  - New Phytologist
VL  - 223
IS  - 3
SP  - 1420-1432
EP  - 1420-1432
PB  - Blackwell Science
SN  - 0028646X
KW  - anthranilic acid (AA)
KW  - Arabidopsis thaliana
KW  - auxin transport
KW  - PIN polarity
KW  - PIN-FORMED proteins
KW  - root gravitropism
UR  - https://nph.onlinelibrary.wiley.com/doi/full/10.1111/nph.15877
L2  - https://nph.onlinelibrary.wiley.com/doi/full/10.1111/nph.15877
N2  - distribution of auxin within plant tissues is of great importance for developmental plasticity, including root gravitropic growth. Auxin flow is directed by the subcellular polar distribution and dynamic relocalisation of auxin transporters such as the PIN-FORMED (PIN) efflux carriers, which can be influenced by the main natural plant auxin indole-3-acetic acid (IAA). Anthranilic acid (AA) is an important early precursor of IAA and previously published studies with AA analogues have suggested that AA may also regulate PIN localisation. Using Arabidopsis thaliana as a model species, we studied an AA-deficient mutant displaying agravitropic root growth, treated seedlings with AA and AA analogues and transformed lines to over-produce AA while inhibiting its conversion to downstream IAA precursors. We showed that AA rescues root gravitropic growth in the AA-deficient mutant at concentrations that do not rescue IAA levels. Overproduction of AA affects root gravitropism without affecting IAA levels. Treatments with, or deficiency in, AA result in defects in PIN polarity and gravistimulus-induced PIN relocalisation in root cells. Our results revealed a previously unknown role for AA in the regulation of PIN subcellular localisation and dynamics involved in root gravitropism, which is independent of its better known role in IAA biosynthesis.
ER  -

DOYLE, S.M., A. RIGAL, P. GRONES, M. KARADY, D.K. BARANGE, M. MAJDA, B. PARIZKOVA, M. KARAMPELIAS, Marta ZWIEWKA, A. PENCIK, F. ALMQVIST, K. LJUNG, O. NOVAK and S. ROBERT. A role for the auxin precursor anthranilic acid in root gravitropism via regulation of PIN-FORMED protein polarity and relocalisation in Arabidopsis. \textit{New Phytologist}. HOBOKEN: Blackwell Science, 2019, vol.~223, No~3, p.~1420-1432. ISSN~0028-646X. Available from: https://dx.doi.org/10.1111/nph.15877.

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