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@article{1636928, author = {Vaneckova, T. and Bezdekova, J. and Tvrdoňová, Michaela and Vlcnovska, M. and Novotna, V. and Neuman, J. and Štossová, Aneta and Kanický, Viktor and Adam, V. and Vaculovicova, M. and Vaculovič, Tomáš}, article_location = {LONDON}, article_number = {AUG}, doi = {http://dx.doi.org/10.1038/s41598-019-48290-2}, keywords = {MAGNETIC NANOPARTICLES; SENSITIVE DETECTION; SURFACE-CHEMISTRY; RECOGNITION; SENSOR; EXTRACTION; SEPARATION; DNA}, language = {eng}, issn = {2045-2322}, journal = {Scientific reports}, title = {CdS quantum dots-based immunoassay combined with particle imprinted polymer technology and laser ablation ICP-MS as a versatile tool for protein detection}, url = {https://www.nature.com/articles/s41598-019-48290-2#Ack1}, volume = {9}, year = {2019} }
TY - JOUR ID - 1636928 AU - Vaneckova, T. - Bezdekova, J. - Tvrdoňová, Michaela - Vlcnovska, M. - Novotna, V. - Neuman, J. - Štossová, Aneta - Kanický, Viktor - Adam, V. - Vaculovicova, M. - Vaculovič, Tomáš PY - 2019 TI - CdS quantum dots-based immunoassay combined with particle imprinted polymer technology and laser ablation ICP-MS as a versatile tool for protein detection JF - Scientific reports VL - 9 IS - AUG SP - 1-9 EP - 1-9 PB - NATURE PUBLISHING GROUP SN - 20452322 KW - MAGNETIC NANOPARTICLES KW - SENSITIVE DETECTION KW - SURFACE-CHEMISTRY KW - RECOGNITION KW - SENSOR KW - EXTRACTION KW - SEPARATION KW - DNA UR - https://www.nature.com/articles/s41598-019-48290-2#Ack1 L2 - https://www.nature.com/articles/s41598-019-48290-2#Ack1 N2 - For the first time, the combination of molecularly imprinted polymer (MIP) technology with laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) is presented with focus on an optimization of the LA-ICP-MS parameters such as laser beam diameter, laser beam fluence, and scan speed using CdS quantum dots (QDs) as a template and dopamine as a functional monomer. A non-covalent imprinting approach was employed in this study due to the simplicity of preparation. Simple oxidative polymerization of the dopamine that creates the self-assembly monolayer seems to be an ideal choice. The QDs prepared by UV light irradiation synthesis were stabilized by using mercaptosuccinic acid. Formation of a complex of QD-anti body and QD-antibody-antigen was verified by using capillary electrophoresis with laser-induced fluorescence detection. QDs and antibody were connected together via an affinity peptide linker. LA-ICP-MS was employed as a proof-of-concept for detection method of two types of immunoassay: 1) antigen extracted from the sample by MIP and subsequently overlaid/immunoreacted by QD-labelled antibodies, 2) complex of antigen, antibody, and QD formed in the sample and subsequently extracted by MIP. The first approach provided higher sensitivity (MIP/NIP), however, the second demonstrated higher selectivity. A mixture of proteins with size in range 10-250 kDa was used as a model sample to demonstrate the capability of both approaches for detection of IgG in a complex sample. ER -
VANECKOVA, T., J. BEZDEKOVA, Michaela TVRDOŇOVÁ, M. VLCNOVSKA, V. NOVOTNA, J. NEUMAN, Aneta ŠTOSSOVÁ, Viktor KANICKÝ, V. ADAM, M. VACULOVICOVA and Tomáš VACULOVIČ. CdS quantum dots-based immunoassay combined with particle imprinted polymer technology and laser ablation ICP-MS as a versatile tool for protein detection. \textit{Scientific reports}. LONDON: NATURE PUBLISHING GROUP, 2019, vol.~9, AUG, p.~1-9. ISSN~2045-2322. Available from: https://dx.doi.org/10.1038/s41598-019-48290-2.
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