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@article{1636956,
author = {Vaneckova, T. and Vanickova, L. and Tvrdoňová, Michaela and Pomorski, A. and Krezel, A. and Vaculovič, Tomáš and Kanický, Viktor and Vaculovicova, M. and Adam, V.},
article_location = {AMSTERDAM},
article_number = {JUN},
doi = {http://dx.doi.org/10.1016/j.talanta.2019.01.089},
keywords = {Metallothionein; Molecularly imprinted polymers; Polydopamine; MALDI-TOP-MS; LA-ICP-MS},
language = {eng},
issn = {0039-9140},
journal = {Talanta},
title = {Molecularly imprinted polymers coupled to mass spectrometric detection for metallothionein sensing},
url = {https://www.sciencedirect.com/science/article/pii/S0039914019301109?via%3Dihub},
volume = {198},
year = {2019}
}
TY - JOUR
ID - 1636956
AU - Vaneckova, T. - Vanickova, L. - Tvrdoňová, Michaela - Pomorski, A. - Krezel, A. - Vaculovič, Tomáš - Kanický, Viktor - Vaculovicova, M. - Adam, V.
PY - 2019
TI - Molecularly imprinted polymers coupled to mass spectrometric detection for metallothionein sensing
JF - Talanta
VL - 198
IS - JUN
SP - 224-229
EP - 224-229
PB - Elsevier
SN - 00399140
KW - Metallothionein
KW - Molecularly imprinted polymers
KW - Polydopamine
KW - MALDI-TOP-MS
KW - LA-ICP-MS
UR - https://www.sciencedirect.com/science/article/pii/S0039914019301109?via%3Dihub
L2 - https://www.sciencedirect.com/science/article/pii/S0039914019301109?via%3Dihub
N2 - We report a facile method for detection of metallothionein (MT), a promising clinically relevant biomarker, in spiked plasma samples. This method, for the first time, integrates molecularly imprinted polymers as purification/pretreatment step with matrix assisted laser desorption/ionization time-of-flight mass spectrometric detection and with laser ablation inductively coupled plasma mass spectrometry for analysis of MTs. The prepared MT-imprinted polydopamine layer showed high binding capacity and specific recognition properties toward the template. Optimal monomer (dopamine) concentration was found to be 16 mM of dopamine. This experimental setup allows to measure mu M concentrations of MT that are present in blood as this can be used for clinical studies recognizing MT as marker of various diseases including tumour one. Presented approach not only provides fast sample throughput but also avoids the limitations of methods based on use of antibodies (e.g. high price, cross reactivity, limited availability in some cases, etc.).
ER -
VANECKOVA, T., L. VANICKOVA, Michaela TVRDOŇOVÁ, A. POMORSKI, A. KREZEL, Tomáš VACULOVIČ, Viktor KANICKÝ, M. VACULOVICOVA and V. ADAM. Molecularly imprinted polymers coupled to mass spectrometric detection for metallothionein sensing. \textit{Talanta}. AMSTERDAM: Elsevier, 2019, vol.~198, JUN, p.~224-229. ISSN~0039-9140. Available from: https://dx.doi.org/10.1016/j.talanta.2019.01.089.
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