SLAVIK, Jan, Josef SKOPALIK, Ivo PROVAZNÍK and Jaromir HUBALEK. Multi-Electrode Array with a Planar Surface for Cell Patterning by Microprinting. Sensors. Basel: MDPI, 2019, vol. 19, No 24, p. 1-11. ISSN 1424-8220. Available from: https://dx.doi.org/10.3390/s19245379. |
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@article{1637257, author = {Slavik, Jan and Skopalik, Josef and Provazník, Ivo and Hubalek, Jaromir}, article_location = {Basel}, article_number = {24}, doi = {http://dx.doi.org/10.3390/s19245379}, keywords = {multielectrode array; microelectrode array; sacrificial layer; peel-off; HL-1 cells; microprinting; antifouling agent}, language = {eng}, issn = {1424-8220}, journal = {Sensors}, title = {Multi-Electrode Array with a Planar Surface for Cell Patterning by Microprinting}, url = {http://dx.doi.org/10.3390/s19245379}, volume = {19}, year = {2019} }
TY - JOUR ID - 1637257 AU - Slavik, Jan - Skopalik, Josef - Provazník, Ivo - Hubalek, Jaromir PY - 2019 TI - Multi-Electrode Array with a Planar Surface for Cell Patterning by Microprinting JF - Sensors VL - 19 IS - 24 SP - 1-11 EP - 1-11 PB - MDPI SN - 14248220 KW - multielectrode array KW - microelectrode array KW - sacrificial layer KW - peel-off KW - HL-1 cells KW - microprinting KW - antifouling agent UR - http://dx.doi.org/10.3390/s19245379 L2 - http://dx.doi.org/10.3390/s19245379 N2 - Multielectrode arrays (MEAs) are devices for non-invasive electrophysiological measurements of cell populations. This paper describes a novel fabrication method of MEAs with a fully planar surface. The surface of the insulation layer and the surface of the electrodes were on one plane; we named this device the planar MEA (pMEA). The main advantage of the pMEA is that it allows uniform contact between the pMEA surface and a substrate for positioning of microfluidic channels or microprinting of a cell adhesive layer. The fabrication of the pMEA is based on a low adhesive Au sacrificial peel-off layer. In divergence from conventional MEAs with recessed electrodes, the electrodes of the pMEA lead across the sloped edge of the insulation layer. To make this, the profile of the edge of the insulation layer was measured and the impedance of the planar electrodes was characterized. The impedance of the pMEA was comparable with the impedance of conventional MEA electrodes. The pMEA was tested for patterning HL-1 cells with a combination of imprinting fibronectin and coating by antifouling poly (l-lysine)-graft-poly(ethylene glycol) (PLL-g-PEG). The HL-1 cells remained patterned even at full confluency and presented spontaneous and synchronous beating activity. ER -
SLAVIK, Jan, Josef SKOPALIK, Ivo PROVAZNÍK and Jaromir HUBALEK. Multi-Electrode Array with a Planar Surface for Cell Patterning by Microprinting. \textit{Sensors}. Basel: MDPI, 2019, vol.~19, No~24, p.~1-11. ISSN~1424-8220. Available from: https://dx.doi.org/10.3390/s19245379.
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