KEZAR, A., L. KAVCIC, Martin POLÁK, Jiří NOVÁČEK, I. GUTIERREZ-AGUIRRE, M.T. ZNIDARIC, A. CO, K. STARE, K. GRUDEN, M. RAVNIKAR, D. PAHOVNIK, E. ZAGAR, F. MERZEL, G. ANDERLUH and M. PODOBNIK. Structural basis for the multitasking nature of the potato virus Y coat protein. Science advances. New York: American Association for the Advancement of Science, 2019, vol. 5, No 7, p. 1-13. ISSN 2375-2548. Available from: https://dx.doi.org/10.1126/sciadv.aaw3808. |
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@article{1637362, author = {Kezar, A. and Kavcic, L. and Polák, Martin and Nováček, Jiří and GutierrezandAguirre, I. and Znidaric, M.T. and Co, A. and Stare, K. and Gruden, K. and Ravnikar, M. and Pahovnik, D. and Zagar, E. and Merzel, F. and Anderluh, G. and Podobnik, M.}, article_location = {New York}, article_number = {7}, doi = {http://dx.doi.org/10.1126/sciadv.aaw3808}, keywords = {REAL-TIME PCR; CRYO-EM; PLANT-VIRUSES; POTYVIRUS; TERMINUS; ELECTROSTATICS; REFINEMENT; RESOLUTION; RESIDUES; PVYNTN}, language = {eng}, issn = {2375-2548}, journal = {Science advances}, title = {Structural basis for the multitasking nature of the potato virus Y coat protein}, url = {https://advances.sciencemag.org/content/advances/5/7/eaaw3808.full.pdf}, volume = {5}, year = {2019} }
TY - JOUR ID - 1637362 AU - Kezar, A. - Kavcic, L. - Polák, Martin - Nováček, Jiří - Gutierrez-Aguirre, I. - Znidaric, M.T. - Co, A. - Stare, K. - Gruden, K. - Ravnikar, M. - Pahovnik, D. - Zagar, E. - Merzel, F. - Anderluh, G. - Podobnik, M. PY - 2019 TI - Structural basis for the multitasking nature of the potato virus Y coat protein JF - Science advances VL - 5 IS - 7 SP - 1-13 EP - 1-13 PB - American Association for the Advancement of Science SN - 23752548 KW - REAL-TIME PCR KW - CRYO-EM KW - PLANT-VIRUSES KW - POTYVIRUS KW - TERMINUS KW - ELECTROSTATICS KW - REFINEMENT KW - RESOLUTION KW - RESIDUES KW - PVYNTN UR - https://advances.sciencemag.org/content/advances/5/7/eaaw3808.full.pdf L2 - https://advances.sciencemag.org/content/advances/5/7/eaaw3808.full.pdf N2 - Potato virus Y (PVY) is among the most economically important plant pathogens. Using cryoelectron microscopy, we determined the near-atomic structure of PVY's flexuous virions, revealing a previously unknown lumenal interplay between extended carboxyl-terminal regions of the coat protein units and viral RNA. RNA-coat protein interactions are crucial for the helical configuration and stability of the virion, as revealed by the unique near-atomic structure of RNA-free virus-like particles. The structures offer the first evidence for plasticity of the coat protein's amino- and carboxyl-terminal regions. Together with mutational analysis and in planta experiments, we show their crucial role in PVY infectivity and explain the ability of the coat protein to perform multiple biological tasks. Moreover, the high modularity of PVY virus-like particles suggests their potential as a new molecular scaffold for nanobiotechnological applications. ER -
KEZAR, A., L. KAVCIC, Martin POLÁK, Jiří NOVÁČEK, I. GUTIERREZ-AGUIRRE, M.T. ZNIDARIC, A. CO, K. STARE, K. GRUDEN, M. RAVNIKAR, D. PAHOVNIK, E. ZAGAR, F. MERZEL, G. ANDERLUH and M. PODOBNIK. Structural basis for the multitasking nature of the potato virus Y coat protein. \textit{Science advances}. New York: American Association for the Advancement of Science, 2019, vol.~5, No~7, p.~1-13. ISSN~2375-2548. Available from: https://dx.doi.org/10.1126/sciadv.aaw3808.
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