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@article{1637377,
author = {Axmann, M. and Sezgin, E. and Karner, A. and Nováček, Jiří and Brodesser, M.D. and Rohrl, C. and Preiner, J. and Stangl, H. and Plochberger, B.},
article_location = {WASHINGTON},
article_number = {4},
doi = {http://dx.doi.org/10.1021/acs.nanolett.9b00319},
keywords = {Low density lipoprotein; (high-speed) atomic force microscopy; fluorescence (cross) correlation spectroscopy; single-molecule-sensitive imaging; cryo-electron microscopy; cholesterol transfer},
language = {eng},
issn = {1530-6984},
journal = {NANO LETTERS},
title = {Receptor-Independent Transfer of Low Density Lipoprotein Cargo to Biomembranes},
url = {https://pubs.acs.org/doi/pdf/10.1021/acs.nanolett.9b00319},
volume = {19},
year = {2019}
}
TY - JOUR
ID - 1637377
AU - Axmann, M. - Sezgin, E. - Karner, A. - Nováček, Jiří - Brodesser, M.D. - Rohrl, C. - Preiner, J. - Stangl, H. - Plochberger, B.
PY - 2019
TI - Receptor-Independent Transfer of Low Density Lipoprotein Cargo to Biomembranes
JF - NANO LETTERS
VL - 19
IS - 4
SP - 2562-2567
EP - 2562-2567
PB - AMER CHEMICAL SOC
SN - 15306984
KW - Low density lipoprotein
KW - (high-speed) atomic force microscopy
KW - fluorescence (cross) correlation spectroscopy
KW - single-molecule-sensitive imaging
KW - cryo-electron microscopy
KW - cholesterol transfer
UR - https://pubs.acs.org/doi/pdf/10.1021/acs.nanolett.9b00319
L2 - https://pubs.acs.org/doi/pdf/10.1021/acs.nanolett.9b00319
N2 - The fundamental task of lipoprotein particles is extracellular transport of cholesterol, lipids, and fatty acids. Besides, cholesterol-rich apoB-containing lipoprotein particles (i.e., low density lipoprotein LDL) are key players in progression of atherosclerotic cardiovascular disease and are associated with familial hypercholesterolemia (FH). So far, lipoprotein particle binding to the cell membrane and subsequent cargo transfer is directly linked to the lipoprotein receptors on the target cell surface. However, our observations showed that lipoprotein particle cargo transport takes place even in the absence of the receptor. This finding suggests that an alternative mechanism for lipoprotein-particle/membrane interaction, besides the receptor-mediated one, exists. Here, we combined several complementary biophysical techniques to obtain a comprehensive view on the nonreceptor mediated LDL-particle/membrane. We applied a combination of atomic force and single-molecule-sensitive fluorescence microscopy (AFM and SMFM) to investigate the LDL particle interaction with membranes of increasing complexity. We observed direct transfer of fluorescently labeled amphiphilic lipid molecules from LDL particles into the pure lipid bilayer. We further confirmed cargo transfer by fluorescence cross-correlation spectroscopy (FCCS) and spectral imaging of environment-sensitive probes. Moreover, the integration of the LDL particle into the membranes was directly visualized by high-speed atomic force microscopy (HS-AFM) and cryo-electron microscopy (cryo-EM). Overall, our data show that lipoprotein particles are able to incorporate into lipid membranes upon contact to transfer their cargo in the absence of specific receptors.
ER -
AXMANN, M., E. SEZGIN, A. KARNER, Jiří NOVÁČEK, M.D. BRODESSER, C. ROHRL, J. PREINER, H. STANGL and B. PLOCHBERGER. Receptor-Independent Transfer of Low Density Lipoprotein Cargo to Biomembranes. \textit{NANO LETTERS}. WASHINGTON: AMER CHEMICAL SOC, 2019, vol.~19, No~4, p.~2562-2567. ISSN~1530-6984. Available from: https://dx.doi.org/10.1021/acs.nanolett.9b00319.
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