J 2020

Bacteriocin production by mucosal bacteria in current and previous colorectal neoplasia

KOHOUTOVA, Darina, Miroslava FORSTLOVA, Paula MORAVKOVA, Jiri CYRANY, Juraj BOSÁK et. al.

Basic information

Original name

Bacteriocin production by mucosal bacteria in current and previous colorectal neoplasia

Authors

KOHOUTOVA, Darina (203 Czech Republic, guarantor), Miroslava FORSTLOVA (203 Czech Republic), Paula MORAVKOVA (203 Czech Republic), Jiri CYRANY (203 Czech Republic), Juraj BOSÁK (703 Slovakia, belonging to the institution), David ŠMAJS (203 Czech Republic, belonging to the institution), Stanislav REJCHRT (203 Czech Republic) and Jan BURES (203 Czech Republic)

Edition

BMC Cancer, LONDON, BMC, 2020, 1471-2407

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30204 Oncology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 4.430

RIV identification code

RIV/00216224:14110/20:00115439

Organization unit

Faculty of Medicine

DOI

UT WoS

000513693200001

Keywords in English

Gramnegative bacteria; Colicin; Microcin; Colorectal neoplasia; Colorectal carcinoma

Tags

Tags

International impact, Reviewed
Změněno: 12/5/2021 12:57, Mgr. Tereza Miškechová

Abstract

V originále

Background Optimal therapy for colorectal carcinoma (CRC), a frequently diagnosed malignancy, does not exist. Some of colicins and microcins, ribosomally synthesized peptides by gramnegative bacteria, have shown significant biological activity specifically against different cancer cells in vitro and in vivo conditions. The aim of this prospective study was to evaluate natural colicin and microcin production by large intestinal mucosal bacteria in each stage of colorectal neoplasia and in those with a history of colorectal neoplasia. Methods A total of 21 patients with non-advanced adenoma (non-a-A; 16/21 with current and 5/21 with history of non-a-A), 20 patients with advanced colorectal adenoma (a-A; 11/20 with current and 9/20 with history of a-A), 22 individuals with CRC (9/22 with current and 13/22 with history of CRC) and 20 controls were enrolled. Mucosal biopsies from the caecum, transverse colon and the rectum were taken during colonoscopy in each individual. Microbiological culture followed. Production of colicins and microcins was evaluated by PCR methods. Results A total of 239 mucosal biopsies were taken. Production of colicins and microcins was significantly more frequent in individuals with non-a-A, a-A and CRC compared to controls. No significant difference in colicin and microcin production was found between patients with current and previous non-a-A, a-A and CRC. Significantly more frequent production of colicins was observed in men compared to women at the stage of colorectal carcinoma. A later onset of increased production of microcins during the adenoma-carcinoma sequence has been observed in males compared to females. Conclusions Strains isolated from large intestinal mucosa in patients with colorectal neoplasia produce colicins and microcins more frequently compared to controls. Bacteriocin production does not differ between patients with current and previous colorectal neoplasia. Fundamental differences in bacteriocin production have been confirmed between males and females.