FISCHER, M., S.E. BELANGER, P. BERCKMANS, M.J. BERNHARD, Luděk BLÁHA, D.E.C. SCHMID, S.D. DYER, T. HAUPT, J.L.M. HERMENS, M.T. HULTMAN, H. LAUE, A. LILLICRAP, Marie MLNAŘÍKOVÁ, A. NATSCH, Jiří NOVÁK, T.L. SINNIGE, K.E. TOLLEFSEN, V. NIEDERHAUSERN VON, H. WITTERS, A. ZUPANIC and K. SCHIRMER. Repeatability and Reproducibility of the RTgill-W1 Cell Line Assay for Predicting Fish Acute Toxicity. Toxicological sciences. OXFORD: Academic Press, 2019, vol. 169, No 2, p. 353-364. ISSN 1096-6080. doi:10.1093/toxsci/kfz057.
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Basic information
Original name Repeatability and Reproducibility of the RTgill-W1 Cell Line Assay for Predicting Fish Acute Toxicity
Authors FISCHER, M. (756 Switzerland), S.E. BELANGER, P. BERCKMANS (840 United States of America), M.J. BERNHARD (56 Belgium), Luděk BLÁHA (203 Czech Republic, guarantor, belonging to the institution), D.E.C. SCHMID (756 Switzerland), S.D. DYER (840 United States of America), T. HAUPT (756 Switzerland), J.L.M. HERMENS, M.T. HULTMAN, H. LAUE, A. LILLICRAP, Marie MLNAŘÍKOVÁ (203 Czech Republic, belonging to the institution), A. NATSCH, Jiří NOVÁK (203 Czech Republic, belonging to the institution), T.L. SINNIGE, K.E. TOLLEFSEN, V. NIEDERHAUSERN VON, H. WITTERS, A. ZUPANIC and K. SCHIRMER.
Edition Toxicological sciences, OXFORD, Academic Press, 2019, 1096-6080.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 30108 Toxicology
Country of publisher United Kingdom of Great Britain and Northern Ireland
Confidentiality degree is not subject to a state or trade secret
WWW URL
Impact factor Impact factor: 3.703
RIV identification code RIV/00216224:14310/19:00113350
Organization unit Faculty of Science
Doi http://dx.doi.org/10.1093/toxsci/kfz057
UT WoS 000475895000004
Keywords in English in vitro alternatives; round-robin study; validation
Tags rivok
Tags International impact, Reviewed
Changed by Changed by: Mgr. Marie Šípková, DiS., učo 437722. Changed: 31/3/2020 11:58.
Abstract
Predicting fish acute toxicity of chemicals in vitro is an attractive alternative method to the conventional approach using juvenile and adult fish. The rainbow trout (Oncorhynchus mykiss) cell line assay with RTgill-W1 cells has been designed for this purpose. It quantifies cell viability using fluorescent measurements for metabolic activity, cell-and lysosomal-membrane integrity on the same set of cells. Results from over 70 organic chemicals attest to the high predictive capacity of this test. We here report on the repeatability (intralaboratory variability) and reproducibility (interlaboratory variability) of the RTgill-W1 cell line assay in a round-robin study focusing on 6 test chemicals involving 6 laboratories from the industrial and academic sector. All participating laboratories were able to establish the assay according to preset quality criteria even though, apart from the lead laboratory, none had previously worked with the RTgill-W1 cell line. Concentration-response modeling, based on either nominal or geometric mean-derived measured concentrations, yielded effect concentrations (EC50) that spanned approximately 4 orders of magnitude over the chemical range, covering all fish acute toxicity categories. Coefficients of variation for intralaboratory and interlaboratory variability for the average of the 3 fluorescent cell viability measurements were 15.5% and 30.8%, respectively, which is comparable to other fish-derived, small-scale bioassays. This study therefore underlines the robustness of the RTgill-W1 cell line assay and its accurate performance when carried out by operators in different laboratory settings.
Links
EF16_013/0001761, research and development projectName: RECETOX RI
LM2015051, research and development projectName: Centrum pro výzkum toxických látek v prostředí (Acronym: RECETOX RI)
Investor: Ministry of Education, Youth and Sports of the CR, Large Infrastructures for Research, Development and Innovation
PrintDisplayed: 26/10/2021 11:04