Repeatability and Reproducibility of the RTgill-W1 Cell Line
Assay for Predicting Fish Acute Toxicity

J 2019

Repeatability and Reproducibility of the RTgill-W1 Cell Line Assay for Predicting Fish Acute Toxicity

FISCHER, M., S.E. BELANGER, P. BERCKMANS, M.J. BERNHARD, Luděk BLÁHA et. al.

Basic information

Original name

Repeatability and Reproducibility of the RTgill-W1 Cell Line Assay for Predicting Fish Acute Toxicity

Authors

FISCHER, M. (756 Switzerland), S.E. BELANGER, P. BERCKMANS (840 United States of America), M.J. BERNHARD (56 Belgium), Luděk BLÁHA (203 Czech Republic, guarantor, belonging to the institution), D.E.C. SCHMID (756 Switzerland), S.D. DYER (840 United States of America), T. HAUPT (756 Switzerland), J.L.M. HERMENS, M.T. HULTMAN, H. LAUE, A. LILLICRAP, Marie MLNAŘÍKOVÁ (203 Czech Republic, belonging to the institution), A. NATSCH, Jiří NOVÁK (203 Czech Republic, belonging to the institution), T.L. SINNIGE, K.E. TOLLEFSEN, V. NIEDERHAUSERN VON, H. WITTERS, A. ZUPANIC and K. SCHIRMER

Edition

Toxicological sciences, OXFORD, Academic Press, 2019, 1096-6080

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30108 Toxicology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 3.703

RIV identification code

RIV/00216224:14310/19:00113350

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1093/toxsci/kfz057

UT WoS

000475895000004

Keywords in English

in vitro alternatives; round-robin study; validation

Abstract

V originále

Predicting fish acute toxicity of chemicals in vitro is an attractive alternative method to the conventional approach using juvenile and adult fish. The rainbow trout (Oncorhynchus mykiss) cell line assay with RTgill-W1 cells has been designed for this purpose. It quantifies cell viability using fluorescent measurements for metabolic activity, cell-and lysosomal-membrane integrity on the same set of cells. Results from over 70 organic chemicals attest to the high predictive capacity of this test. We here report on the repeatability (intralaboratory variability) and reproducibility (interlaboratory variability) of the RTgill-W1 cell line assay in a round-robin study focusing on 6 test chemicals involving 6 laboratories from the industrial and academic sector. All participating laboratories were able to establish the assay according to preset quality criteria even though, apart from the lead laboratory, none had previously worked with the RTgill-W1 cell line. Concentration-response modeling, based on either nominal or geometric mean-derived measured concentrations, yielded effect concentrations (EC50) that spanned approximately 4 orders of magnitude over the chemical range, covering all fish acute toxicity categories. Coefficients of variation for intralaboratory and interlaboratory variability for the average of the 3 fluorescent cell viability measurements were 15.5% and 30.8%, respectively, which is comparable to other fish-derived, small-scale bioassays. This study therefore underlines the robustness of the RTgill-W1 cell line assay and its accurate performance when carried out by operators in different laboratory settings.

Links

EF16_013/0001761, research and development project

Name: RECETOX RI

LM2015051, research and development project

Name: Centrum pro výzkum toxických látek v prostředí (Acronym: RECETOX RI)

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

FISCHER, M., S.E. BELANGER, P. BERCKMANS, M.J. BERNHARD, Luděk BLÁHA, D.E.C. SCHMID, S.D. DYER, T. HAUPT, J.L.M. HERMENS, M.T. HULTMAN, H. LAUE, A. LILLICRAP, Marie MLNAŘÍKOVÁ, A. NATSCH, Jiří NOVÁK, T.L. SINNIGE, K.E. TOLLEFSEN, V. NIEDERHAUSERN VON, H. WITTERS, A. ZUPANIC and K. SCHIRMER. Repeatability and Reproducibility of the RTgill-W1 Cell Line Assay for Predicting Fish Acute Toxicity. Toxicological sciences. OXFORD: Academic Press, 2019, vol. 169, No 2, p. 353-364. ISSN 1096-6080. Available from: https://dx.doi.org/10.1093/toxsci/kfz057.

@article{1639238,
   author = {Fischer, M. and Belanger, S.E. and Berckmans, P. and Bernhard, M.J. and Bláha, Luděk and Schmid, D.E.C. and Dyer, S.D. and Haupt, T. and Hermens, J.L.M. and Hultman, M.T. and Laue, H. and Lillicrap, A. and Mlnaříková, Marie and Natsch, A. and Novák, Jiří and Sinnige, T.L. and Tollefsen, K.E. and Niederhausern von, V. and Witters, H. and Zupanic, A. and Schirmer, K.},
   article_location = {OXFORD},
   article_number = {2},
   doi = {http://dx.doi.org/10.1093/toxsci/kfz057},
   keywords = {in vitro alternatives; round-robin study; validation},
   language = {eng},
   issn = {1096-6080},
   journal = {Toxicological sciences},
   title = {Repeatability and Reproducibility of the RTgill-W1 Cell Line Assay for Predicting Fish Acute Toxicity},
   url = {http://dx.doi.org/10.1093/toxsci/kfz057},
   volume = {169},
   year = {2019}
}

TY  - JOUR
ID  - 1639238
AU  - Fischer, M. - Belanger, S.E. - Berckmans, P. - Bernhard, M.J. - Bláha, Luděk - Schmid, D.E.C. - Dyer, S.D. - Haupt, T. - Hermens, J.L.M. - Hultman, M.T. - Laue, H. - Lillicrap, A. - Mlnaříková, Marie - Natsch, A. - Novák, Jiří - Sinnige, T.L. - Tollefsen, K.E. - Niederhausern von, V. - Witters, H. - Zupanic, A. - Schirmer, K.
PY  - 2019
TI  - Repeatability and Reproducibility of the RTgill-W1 Cell Line Assay for Predicting Fish Acute Toxicity
JF  - Toxicological sciences
VL  - 169
IS  - 2
SP  - 353-364
EP  - 353-364
PB  - Academic Press
SN  - 10966080
KW  - in vitro alternatives
KW  - round-robin study
KW  - validation
UR  - http://dx.doi.org/10.1093/toxsci/kfz057
L2  - http://dx.doi.org/10.1093/toxsci/kfz057
N2  - Predicting fish acute toxicity of chemicals in vitro is an attractive alternative method to the conventional approach using juvenile and adult fish. The rainbow trout (Oncorhynchus mykiss) cell line assay with RTgill-W1 cells has been designed for this purpose. It quantifies cell viability using fluorescent measurements for metabolic activity, cell-and lysosomal-membrane integrity on the same set of cells. Results from over 70 organic chemicals attest to the high predictive capacity of this test. We here report on the repeatability (intralaboratory variability) and reproducibility (interlaboratory variability) of the RTgill-W1 cell line assay in a round-robin study focusing on 6 test chemicals involving 6 laboratories from the industrial and academic sector. All participating laboratories were able to establish the assay according to preset quality criteria even though, apart from the lead laboratory, none had previously worked with the RTgill-W1 cell line. Concentration-response modeling, based on either nominal or geometric mean-derived measured concentrations, yielded effect concentrations (EC50) that spanned approximately 4 orders of magnitude over the chemical range, covering all fish acute toxicity categories. Coefficients of variation for intralaboratory and interlaboratory variability for the average of the 3 fluorescent cell viability measurements were 15.5% and 30.8%, respectively, which is comparable to other fish-derived, small-scale bioassays. This study therefore underlines the robustness of the RTgill-W1 cell line assay and its accurate performance when carried out by operators in different laboratory settings.
ER  -

FISCHER, M., S.E. BELANGER, P. BERCKMANS, M.J. BERNHARD, Luděk BLÁHA, D.E.C. SCHMID, S.D. DYER, T. HAUPT, J.L.M. HERMENS, M.T. HULTMAN, H. LAUE, A. LILLICRAP, Marie MLNAŘÍKOVÁ, A. NATSCH, Jiří NOVÁK, T.L. SINNIGE, K.E. TOLLEFSEN, V. NIEDERHAUSERN VON, H. WITTERS, A. ZUPANIC and K. SCHIRMER. Repeatability and Reproducibility of the RTgill-W1 Cell Line Assay for Predicting Fish Acute Toxicity. \textit{Toxicological sciences}. OXFORD: Academic Press, 2019, vol.~169, No~2, p.~353-364. ISSN~1096-6080. Available from: https://dx.doi.org/10.1093/toxsci/kfz057.

Detailed Information on Publication Record