The effects of nano-sized PbO on biomarkers of membrane
disruption and DNA damage in a sub-chronic inhalation study on
mice

J 2020

The effects of nano-sized PbO on biomarkers of membrane disruption and DNA damage in a sub-chronic inhalation study on mice

BLÁHOVÁ, Lucie, Zuzana NOVÁKOVÁ, Zbyněk VEČEŘA, Lucie VRLÍKOVÁ, Bohumil DOČEKAL et. al.

Basic information

Original name

The effects of nano-sized PbO on biomarkers of membrane disruption and DNA damage in a sub-chronic inhalation study on mice

Authors

BLÁHOVÁ, Lucie (203 Czech Republic, belonging to the institution), Zuzana NOVÁKOVÁ (203 Czech Republic, belonging to the institution), Zbyněk VEČEŘA (203 Czech Republic), Lucie VRLÍKOVÁ (203 Czech Republic), Bohumil DOČEKAL (203 Czech Republic), Jana DUMKOVÁ (203 Czech Republic, belonging to the institution), Kamil KRUMAL (203 Czech Republic), Pavel MIKUSKA (203 Czech Republic), Marcela BUCHTOVÁ (203 Czech Republic, belonging to the institution), Aleš HAMPL (203 Czech Republic, belonging to the institution), Klára HILSCHEROVÁ (203 Czech Republic, belonging to the institution) and Luděk BLÁHA (203 Czech Republic, guarantor, belonging to the institution)

Edition

NANOTOXICOLOGY, ABINGDON, TAYLOR & FRANCIS LTD, 2020, 1743-5390

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30108 Toxicology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 5.913

RIV identification code

RIV/00216224:14310/20:00115464

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1080/17435390.2019.1685696

UT WoS

000496628900001

Keywords in English

Oxidative damage; lead; nanoparticle; 8-OHdG; TBARS; 8-isoPGF2(alpha); 8-isoPGE isoprostanes; LC-MS; MS; lipid peroxidation; inhalation

Abstract

V originále

Although the production of engineered nanoparticles increases our knowledge of toxicity and mechanisms of bioactivity during relevant exposures is lacking. In the present study mice were exposed to PbO nanoparticles (PbONP; 192.5 mu g/m(3); 1.93 x 10(6) particles/cm(3)) for 2, 5 and 13 weeks through continuous inhalation. The analyses addressed Pb and PbONP distribution in organs (lung, liver, kidney, brain) using electrothermal atomic absorption spectrometry and transmission electron microscopy, as well as histopathology and analyses of oxidative stress biomarkers. New LC-MS/MS methods were validated for biomarkers of lipid damage F2-isoprostanes (8-iso-prostaglandins F2-alpha and E-2) and hydroxylated deoxoguanosine (8-OHdG, marker of DNA oxidation). Commonly studied malondialdehyde was also measured as TBARS by HPLC-DAD. The study revealed fast blood transport and distribution of Pb from the lung to the kidney and liver. A different Pb accumulation trend was observed in the brain, suggesting transfer of NP along the nasal nerve to the olfactory bulbs. Long-term inhalation of PbONP caused lipid peroxidation in animal brains (increased levels of TBARS and both isoprostanes). Membrane lipid damage was also detected in the kidney after shorter exposures, but not in the liver or lung. On the contrary, longer exposures to PbONP increased levels of 8-OHdG in the lung and temporarily increased lung weight after 2 and 5 weeks of exposure. The histopathological changes observed mainly in the lung and liver indicated inflammation and general toxicity responses. The present long-term inhalation study indicates risks of PbONP to both human health and the environment.

Links

EF16_013/0001761, research and development project

Name: RECETOX RI

LM2015051, research and development project

Name: Centrum pro výzkum toxických látek v prostředí (Acronym: RECETOX RI)

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

BLÁHOVÁ, Lucie, Zuzana NOVÁKOVÁ, Zbyněk VEČEŘA, Lucie VRLÍKOVÁ, Bohumil DOČEKAL, Jana DUMKOVÁ, Kamil KRUMAL, Pavel MIKUSKA, Marcela BUCHTOVÁ, Aleš HAMPL, Klára HILSCHEROVÁ and Luděk BLÁHA. The effects of nano-sized PbO on biomarkers of membrane disruption and DNA damage in a sub-chronic inhalation study on mice. NANOTOXICOLOGY. ABINGDON: TAYLOR & FRANCIS LTD, 2020, vol. 14, No 2, p. 214-231. ISSN 1743-5390. Available from: https://dx.doi.org/10.1080/17435390.2019.1685696.

@article{1640436,
   author = {Bláhová, Lucie and Nováková, Zuzana and Večeřa, Zbyněk and Vrlíková, Lucie and Dočekal, Bohumil and Dumková, Jana and Krumal, Kamil and Mikuska, Pavel and Buchtová, Marcela and Hampl, Aleš and Hilscherová, Klára and Bláha, Luděk},
   article_location = {ABINGDON},
   article_number = {2},
   doi = {http://dx.doi.org/10.1080/17435390.2019.1685696},
   keywords = {Oxidative damage; lead; nanoparticle; 8-OHdG; TBARS; 8-isoPGF2(alpha); 8-isoPGE isoprostanes; LC-MS; MS; lipid peroxidation; inhalation},
   language = {eng},
   issn = {1743-5390},
   journal = {NANOTOXICOLOGY},
   title = {The effects of nano-sized PbO on biomarkers of membrane disruption and DNA damage in a sub-chronic inhalation study on mice},
   url = {https://www.tandfonline.com/doi/abs/10.1080/17435390.2019.1685696?journalCode=inan20},
   volume = {14},
   year = {2020}
}

TY  - JOUR
ID  - 1640436
AU  - Bláhová, Lucie - Nováková, Zuzana - Večeřa, Zbyněk - Vrlíková, Lucie - Dočekal, Bohumil - Dumková, Jana - Krumal, Kamil - Mikuska, Pavel - Buchtová, Marcela - Hampl, Aleš - Hilscherová, Klára - Bláha, Luděk
PY  - 2020
TI  - The effects of nano-sized PbO on biomarkers of membrane disruption and DNA damage in a sub-chronic inhalation study on mice
JF  - NANOTOXICOLOGY
VL  - 14
IS  - 2
SP  - 214-231
EP  - 214-231
PB  - TAYLOR & FRANCIS LTD
SN  - 17435390
KW  - Oxidative damage
KW  - lead
KW  - nanoparticle
KW  - 8-OHdG
KW  - TBARS
KW  - 8-isoPGF2(alpha)
KW  - 8-isoPGE isoprostanes
KW  - LC-MS
KW  - MS
KW  - lipid peroxidation
KW  - inhalation
UR  - https://www.tandfonline.com/doi/abs/10.1080/17435390.2019.1685696?journalCode=inan20
L2  - https://www.tandfonline.com/doi/abs/10.1080/17435390.2019.1685696?journalCode=inan20
N2  - Although the production of engineered nanoparticles increases our knowledge of toxicity and mechanisms of bioactivity during relevant exposures is lacking. In the present study mice were exposed to PbO nanoparticles (PbONP; 192.5 mu g/m(3); 1.93 x 10(6) particles/cm(3)) for 2, 5 and 13 weeks through continuous inhalation. The analyses addressed Pb and PbONP distribution in organs (lung, liver, kidney, brain) using electrothermal atomic absorption spectrometry and transmission electron microscopy, as well as histopathology and analyses of oxidative stress biomarkers. New LC-MS/MS methods were validated for biomarkers of lipid damage F2-isoprostanes (8-iso-prostaglandins F2-alpha and E-2) and hydroxylated deoxoguanosine (8-OHdG, marker of DNA oxidation). Commonly studied malondialdehyde was also measured as TBARS by HPLC-DAD. The study revealed fast blood transport and distribution of Pb from the lung to the kidney and liver. A different Pb accumulation trend was observed in the brain, suggesting transfer of NP along the nasal nerve to the olfactory bulbs. Long-term inhalation of PbONP caused lipid peroxidation in animal brains (increased levels of TBARS and both isoprostanes). Membrane lipid damage was also detected in the kidney after shorter exposures, but not in the liver or lung. On the contrary, longer exposures to PbONP increased levels of 8-OHdG in the lung and temporarily increased lung weight after 2 and 5 weeks of exposure. The histopathological changes observed mainly in the lung and liver indicated inflammation and general toxicity responses. The present long-term inhalation study indicates risks of PbONP to both human health and the environment.
ER  -

BLÁHOVÁ, Lucie, Zuzana NOVÁKOVÁ, Zbyněk VEČEŘA, Lucie VRLÍKOVÁ, Bohumil DOČEKAL, Jana DUMKOVÁ, Kamil KRUMAL, Pavel MIKUSKA, Marcela BUCHTOVÁ, Aleš HAMPL, Klára HILSCHEROVÁ and Luděk BLÁHA. The effects of nano-sized PbO on biomarkers of membrane disruption and DNA damage in a sub-chronic inhalation study on mice. \textit{NANOTOXICOLOGY}. ABINGDON: TAYLOR \&{} FRANCIS LTD, 2020, vol.~14, No~2, p.~214-231. ISSN~1743-5390. Available from: https://dx.doi.org/10.1080/17435390.2019.1685696.

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