ASLAN-UZEL, Askin S., Andy BEIER, David KOVÁŘ, Clemens CZIEGLER, Santosh K. PADHI, Eva D. SCHUITEN, Mark DORR, Dominique BOTTCHER, Frank HOLLMANN, Florian RUDROFF, Marko D. MIHOVILOVIC, Tomáš BURYŠKA, Jiří DAMBORSKÝ, Zbyněk PROKOP, Christoffel P. S. BADENHORST and Uwe T. BORNSCHEUER. An Ultrasensitive Fluorescence Assay for the Detection of Halides and Enzymatic Dehalogenation. ChemCatChem. WEINHEIM: WILEY-V C H VERLAG GMBH, 2020, vol. 12, No 7, p. 2032-2039. ISSN 1867-3880. Available from: https://dx.doi.org/10.1002/cctc.201901891. |
Other formats:
BibTeX
LaTeX
RIS
@article{1640717, author = {AslanandUzel, Askin S. and Beier, Andy and Kovář, David and Cziegler, Clemens and Padhi, Santosh K. and Schuiten, Eva D. and Dorr, Mark and Bottcher, Dominique and Hollmann, Frank and Rudroff, Florian and Mihovilovic, Marko D. and Buryška, Tomáš and Damborský, Jiří and Prokop, Zbyněk and Badenhorst, Christoffel P. S. and Bornscheuer, Uwe T.}, article_location = {WEINHEIM}, article_number = {7}, doi = {http://dx.doi.org/10.1002/cctc.201901891}, keywords = {dehalogenase; fluorescence; halides; haloalkane; haloperoxidase}, language = {eng}, issn = {1867-3880}, journal = {ChemCatChem}, title = {An Ultrasensitive Fluorescence Assay for the Detection of Halides and Enzymatic Dehalogenation}, url = {https://onlinelibrary.wiley.com/doi/full/10.1002/cctc.201901891}, volume = {12}, year = {2020} }
TY - JOUR ID - 1640717 AU - Aslan-Uzel, Askin S. - Beier, Andy - Kovář, David - Cziegler, Clemens - Padhi, Santosh K. - Schuiten, Eva D. - Dorr, Mark - Bottcher, Dominique - Hollmann, Frank - Rudroff, Florian - Mihovilovic, Marko D. - Buryška, Tomáš - Damborský, Jiří - Prokop, Zbyněk - Badenhorst, Christoffel P. S. - Bornscheuer, Uwe T. PY - 2020 TI - An Ultrasensitive Fluorescence Assay for the Detection of Halides and Enzymatic Dehalogenation JF - ChemCatChem VL - 12 IS - 7 SP - 2032-2039 EP - 2032-2039 PB - WILEY-V C H VERLAG GMBH SN - 18673880 KW - dehalogenase KW - fluorescence KW - halides KW - haloalkane KW - haloperoxidase UR - https://onlinelibrary.wiley.com/doi/full/10.1002/cctc.201901891 L2 - https://onlinelibrary.wiley.com/doi/full/10.1002/cctc.201901891 N2 - Halide assays are important for the study of enzymatic dehalogenation, a topic of great industrial and scientific importance. Here we describe the development of a very sensitive halide assay that can detect less than a picomole of bromide ions, making it very useful for quantifying enzymatic dehalogenation products. Halides are oxidised under mild conditions using the vanadium-dependent chloroperoxidase from Curvularia inaequalis, forming hypohalous acids that are detected using aminophenyl fluorescein. The assay is up to three orders of magnitude more sensitive than currently available alternatives, with detection limits of 20 nM for bromide and 1 mu M for chloride and iodide. We demonstrate that the assay can be used to determine specific activities of dehalogenases and validate this by comparison to a well-established GC-MS method. This new assay will facilitate the identification and characterisation of novel dehalogenases and may also be of interest to those studying other halide-producing enzymes. ER -
ASLAN-UZEL, Askin S., Andy BEIER, David KOVÁŘ, Clemens CZIEGLER, Santosh K. PADHI, Eva D. SCHUITEN, Mark DORR, Dominique BOTTCHER, Frank HOLLMANN, Florian RUDROFF, Marko D. MIHOVILOVIC, Tomáš BURYŠKA, Jiří DAMBORSKÝ, Zbyněk PROKOP, Christoffel P. S. BADENHORST and Uwe T. BORNSCHEUER. An Ultrasensitive Fluorescence Assay for the Detection of Halides and Enzymatic Dehalogenation. \textit{ChemCatChem}. WEINHEIM: WILEY-V C H VERLAG GMBH, 2020, vol.~12, No~7, p.~2032-2039. ISSN~1867-3880. Available from: https://dx.doi.org/10.1002/cctc.201901891.
|