Controlling selectivity of polymer-based monolithic stationary
phases

J 2019

Controlling selectivity of polymer-based monolithic stationary phases

KOMENDOVÁ, Martina, Luiz Fernando RIBEIRO and Jiří URBAN

Basic information

Original name

Controlling selectivity of polymer-based monolithic stationary phases

Authors

KOMENDOVÁ, Martina (203 Czech Republic, belonging to the institution), Luiz Fernando RIBEIRO (76 Brazil) and Jiří URBAN (203 Czech Republic, guarantor, belonging to the institution)

Edition

Journal of separation science, Weinheim, WILEY-V C H VERLAG GMBH, 2019, 1615-9306

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10406 Analytical chemistry

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 2.878

RIV identification code

RIV/00216224:14310/19:00108238

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1002/jssc.201801046

UT WoS

000461674900001

Keywords in English

dopamine; metabolism; polymer monoliths; retention; zwitterions

Abstract

V originále

In this work, we aimed to prepare a monolithic capillary column that allowed an isocratic separation of ten dopamine precursors and metabolites in a single run. Segments of five zwitterion sulfobetaine polymer monoliths have been modified by zwitterion phoshorylcholine by using an ultraviolet-initiated two-step photografting. Columns with 0, 33, 50, 66, and 100% of modified length were prepared. Effect of length of the modified segment and mobile phase composition has been tested. All columns provided dual-retention mechanism with reversed-phase retention in highly aqueous mobile phase and hydrophilic interaction mechanism in highly organic mobile phase. The retention mechanism was controlled by the composition of the mobile phase and has been described by a three-parameter model. We have used regression parameters to characterize the retention of analyzed compounds and to study individual pathways of dopamine metabolism. Comprehensive optimization of mobile phase composition allowed to find an optimal composition of the mobile phase and stationary phase surface chemistry arrangement to achieve desired separation. Optimized columns provided an isocratic separation of all tested compounds in less than nine min.

Links

GA17-11252S, research and development project

Name: Polymerní monolitické stacionární fáze s kontrolovanou selektivitou

Investor: Czech Science Foundation

Citovat

KOMENDOVÁ, Martina, Luiz Fernando RIBEIRO and Jiří URBAN. Controlling selectivity of polymer-based monolithic stationary phases. Journal of separation science. Weinheim: WILEY-V C H VERLAG GMBH, 2019, vol. 42, No 5, p. 952-961. ISSN 1615-9306. Available from: https://dx.doi.org/10.1002/jssc.201801046.

@article{1642101,
   author = {Komendová, Martina and Ribeiro, Luiz Fernando and Urban, Jiří},
   article_location = {Weinheim},
   article_number = {5},
   doi = {http://dx.doi.org/10.1002/jssc.201801046},
   keywords = {dopamine; metabolism; polymer monoliths; retention; zwitterions},
   language = {eng},
   issn = {1615-9306},
   journal = {Journal of separation science},
   title = {Controlling selectivity of polymer-based monolithic stationary phases},
   url = {https://onlinelibrary.wiley.com/doi/full/10.1002/jssc.201801046},
   volume = {42},
   year = {2019}
}

TY  - JOUR
ID  - 1642101
AU  - Komendová, Martina - Ribeiro, Luiz Fernando - Urban, Jiří
PY  - 2019
TI  - Controlling selectivity of polymer-based monolithic stationary phases
JF  - Journal of separation science
VL  - 42
IS  - 5
SP  - 952-961
EP  - 952-961
PB  - WILEY-V C H VERLAG GMBH
SN  - 16159306
KW  - dopamine
KW  - metabolism
KW  - polymer monoliths
KW  - retention
KW  - zwitterions
UR  - https://onlinelibrary.wiley.com/doi/full/10.1002/jssc.201801046
L2  - https://onlinelibrary.wiley.com/doi/full/10.1002/jssc.201801046
N2  - In this work, we aimed to prepare a monolithic capillary column that allowed an isocratic separation of ten dopamine precursors and metabolites in a single run. Segments of five zwitterion sulfobetaine polymer monoliths have been modified by zwitterion phoshorylcholine by using an ultraviolet-initiated two-step photografting. Columns with 0, 33, 50, 66, and 100% of modified length were prepared. Effect of length of the modified segment and mobile phase composition has been tested. All columns provided dual-retention mechanism with reversed-phase retention in highly aqueous mobile phase and hydrophilic interaction mechanism in highly organic mobile phase. The retention mechanism was controlled by the composition of the mobile phase and has been described by a three-parameter model. We have used regression parameters to characterize the retention of analyzed compounds and to study individual pathways of dopamine metabolism. Comprehensive optimization of mobile phase composition allowed to find an optimal composition of the mobile phase and stationary phase surface chemistry arrangement to achieve desired separation. Optimized columns provided an isocratic separation of all tested compounds in less than nine min.
ER  -

KOMENDOVÁ, Martina, Luiz Fernando RIBEIRO and Jiří URBAN. Controlling selectivity of polymer-based monolithic stationary phases. \textit{Journal of separation science}. Weinheim: WILEY-V C H VERLAG GMBH, 2019, vol.~42, No~5, p.~952-961. ISSN~1615-9306. Available from: https://dx.doi.org/10.1002/jssc.201801046.

Detailed Information on Publication Record