LC-MS/MS study of in vivo fate of hyaluronan polymeric micelles
carrying doxorubicin

J 2019

LC-MS/MS study of in vivo fate of hyaluronan polymeric micelles carrying doxorubicin

SIMEK, Matej, Martina HERMANNOVA, Daniela SMEJKALOVA, Tereza FOGLOVA, Karel SOUČEK et. al.

Basic information

Original name

LC-MS/MS study of in vivo fate of hyaluronan polymeric micelles carrying doxorubicin

Authors

SIMEK, Matej, Martina HERMANNOVA (guarantor), Daniela SMEJKALOVA, Tereza FOGLOVA, Karel SOUČEK (203 Czech Republic, belonging to the institution), Lucia BINO and Vladimir VELEBNY

Edition

Carbohydrate Polymers, Oxford, ELSEVIER SCI LTD, 2019, 0144-8617

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10400 1.4 Chemical sciences

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 7.182

RIV identification code

RIV/00216224:14310/19:00113513

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1016/j.carbpol.2018.12.104

UT WoS

000457546200018

Keywords in English

Hyaluronan; Doxorubicin; Polymeric micelles; Biodistribution; Pharmacokinetics

Abstract

V originále

A better understanding of in vivo behavior of nanocarriers is necessary for further improvement in their development. Here we present a novel approach, where both the matrix and the drug can be analyzed by LC-MS/MS after one sample handling. The developed method was applied for the comparison of pharmacokinetic profile of free and encapsulated doxorubicin (DOX) in oleyl hyaluronan (HA-C18:1) polymeric micelles. The results indicated that nanocarriers were rapidly dissociated upon in vivo administration. Despite this fact, the administration of encapsulated DOX led to its longer circulation time and enhanced tumor targeting. This effect was not observed injecting blank HA-C18:1 micelles followed by unencapsulated DOX. Biodistribution studies and molecular weight estimation of the carrier matrix indicated relatively high stability of HA-C18:1 ester bond in bloodstream and complete elimination of the derivative within 72 h. The proposed methodology provides a novel strategy to elucidate the pharmacokinetic behavior of polysaccharide-based drug delivery systems.

Citovat

SIMEK, Matej, Martina HERMANNOVA, Daniela SMEJKALOVA, Tereza FOGLOVA, Karel SOUČEK, Lucia BINO and Vladimir VELEBNY. LC-MS/MS study of in vivo fate of hyaluronan polymeric micelles carrying doxorubicin. Carbohydrate Polymers. Oxford: ELSEVIER SCI LTD, 2019, vol. 209, APR 1 2019, p. 181-189. ISSN 0144-8617. Available from: https://dx.doi.org/10.1016/j.carbpol.2018.12.104.

@article{1642136,
   author = {Simek, Matej and Hermannova, Martina and Smejkalova, Daniela and Foglova, Tereza and Souček, Karel and Bino, Lucia and Velebny, Vladimir},
   article_location = {Oxford},
   article_number = {APR 1 2019},
   doi = {http://dx.doi.org/10.1016/j.carbpol.2018.12.104},
   keywords = {Hyaluronan; Doxorubicin; Polymeric micelles; Biodistribution; Pharmacokinetics},
   language = {eng},
   issn = {0144-8617},
   journal = {Carbohydrate Polymers},
   title = {LC-MS/MS study of in vivo fate of hyaluronan polymeric micelles carrying doxorubicin},
   url = {https://www.sciencedirect.com/science/article/pii/S0144861718315492},
   volume = {209},
   year = {2019}
}

TY  - JOUR
ID  - 1642136
AU  - Simek, Matej - Hermannova, Martina - Smejkalova, Daniela - Foglova, Tereza - Souček, Karel - Bino, Lucia - Velebny, Vladimir
PY  - 2019
TI  - LC-MS/MS study of in vivo fate of hyaluronan polymeric micelles carrying doxorubicin
JF  - Carbohydrate Polymers
VL  - 209
IS  - APR 1 2019
SP  - 181-189
EP  - 181-189
PB  - ELSEVIER SCI LTD
SN  - 01448617
KW  - Hyaluronan
KW  - Doxorubicin
KW  - Polymeric micelles
KW  - Biodistribution
KW  - Pharmacokinetics
UR  - https://www.sciencedirect.com/science/article/pii/S0144861718315492
L2  - https://www.sciencedirect.com/science/article/pii/S0144861718315492
N2  - A better understanding of in vivo behavior of nanocarriers is necessary for further improvement in their development. Here we present a novel approach, where both the matrix and the drug can be analyzed by LC-MS/MS after one sample handling. The developed method was applied for the comparison of pharmacokinetic profile of free and encapsulated doxorubicin (DOX) in oleyl hyaluronan (HA-C18:1) polymeric micelles. The results indicated that nanocarriers were rapidly dissociated upon in vivo administration. Despite this fact, the administration of encapsulated DOX led to its longer circulation time and enhanced tumor targeting. This effect was not observed injecting blank HA-C18:1 micelles followed by unencapsulated DOX. Biodistribution studies and molecular weight estimation of the carrier matrix indicated relatively high stability of HA-C18:1 ester bond in bloodstream and complete elimination of the derivative within 72 h. The proposed methodology provides a novel strategy to elucidate the pharmacokinetic behavior of polysaccharide-based drug delivery systems.
ER  -

SIMEK, Matej, Martina HERMANNOVA, Daniela SMEJKALOVA, Tereza FOGLOVA, Karel SOUČEK, Lucia BINO and Vladimir VELEBNY. LC-MS/MS study of in vivo fate of hyaluronan polymeric micelles carrying doxorubicin. \textit{Carbohydrate Polymers}. Oxford: ELSEVIER SCI LTD, 2019, vol.~209, APR 1 2019, p.~181-189. ISSN~0144-8617. Available from: https://dx.doi.org/10.1016/j.carbpol.2018.12.104.

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