| KRAFČÍKOVÁ, Michaela, R. HANSEL-HERTSCH, Lukáš TRANTÍREK and Silvie TRANTÍRKOVÁ. In Cell NMR Spectroscopy: Investigation of G-Quadruplex Structures Inside Living Xenopus laevis Oocytes. G-QUADRUPLEX NUCLEIC ACIDS: METHODS AND PROTOCOLS. TOTOWA: HUMANA PRESS INC, 2019, vol. 2035, No 2019, p. 397-405. ISSN 1064-3745. Available from: https://dx.doi.org/10.1007/978-1-4939-9666-7_25. |
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@article{1643296,
author = {Krafčíková, Michaela and HanselandHertsch, R. and Trantírek, Lukáš and Trantírková, Silvie},
article_location = {TOTOWA},
article_number = {2019},
doi = {http://dx.doi.org/10.1007/978-1-4939-9666-7_25},
keywords = {In-cell NMR; Xenopus laevis oocyte; DNA; RNA; G-quadruplex},
language = {eng},
issn = {1064-3745},
journal = {G-QUADRUPLEX NUCLEIC ACIDS: METHODS AND PROTOCOLS},
title = {In Cell NMR Spectroscopy: Investigation of G-Quadruplex Structures Inside Living Xenopus laevis Oocytes},
url = {https://link.springer.com/protocol/10.1007/978-1-4939-9666-7_25},
volume = {2035},
year = {2019}
}
TY - JOUR
ID - 1643296
AU - Krafčíková, Michaela - Hansel-Hertsch, R. - Trantírek, Lukáš - Trantírková, Silvie
PY - 2019
TI - In Cell NMR Spectroscopy: Investigation of G-Quadruplex Structures Inside Living Xenopus laevis Oocytes
JF - G-QUADRUPLEX NUCLEIC ACIDS: METHODS AND PROTOCOLS
VL - 2035
IS - 2019
SP - 397-405
EP - 397-405
PB - HUMANA PRESS INC
SN - 10643745
KW - In-cell NMR
KW - Xenopus laevis oocyte
KW - DNA
KW - RNA
KW - G-quadruplex
UR - https://link.springer.com/protocol/10.1007/978-1-4939-9666-7_25
L2 - https://link.springer.com/protocol/10.1007/978-1-4939-9666-7_25
N2 - G-quadruplexes are inherently polymorphic nucleic acid structures. Their folding topology depends on the nucleic acid primary sequence and on physical-chemical environmental factors. Hence, it remains unclear if a G-quadruplex topology determined in the test tube (in vitro) will also form in vivo. Characterization of G-quadruplexes in their native environment has been proposed as an efficient strategy to tackle this issue. So far, characterization of G-quadruplex structures in living cells has relied exclusively on the use of Xenopus laevis oocytes as a eukaryotic cell model system. Here, we describe the protocol for the preparation of X. laevis oocytes for studies of G-quadruplexes as well as other nucleic acids motifs under native conditions using in-cell NMR spectroscopy.
ER -
KRAFČÍKOVÁ, Michaela, R. HANSEL-HERTSCH, Lukáš TRANTÍREK and Silvie TRANTÍRKOVÁ. In Cell NMR Spectroscopy: Investigation of G-Quadruplex Structures Inside Living Xenopus laevis Oocytes. \textit{G-QUADRUPLEX NUCLEIC ACIDS: METHODS AND PROTOCOLS}. TOTOWA: HUMANA PRESS INC, 2019, vol.~2035, No~2019, p.~397-405. ISSN~1064-3745. Available from: https://dx.doi.org/10.1007/978-1-4939-9666-7\_{}25.
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