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@article{1644478,
author = {Nováková, Dana and Švec, Pavel and Zeman, Michal and Busse, HansandJürgen and Mašlaňová, Ivana and Pantůček, Roman and Králová, Stanislava and Krištofová, Lucie and Sedláček, Ivo},
article_location = {London},
article_number = {1},
doi = {http://dx.doi.org/10.1099/ijsem.0.003753},
keywords = {Pseudomonas leptonychotis; Antarctica; Weddell seal; polyphasic taxonomy},
language = {eng},
issn = {1466-5026},
journal = {International Journal of systematic and Evolutionary Microbiology},
title = {Pseudomonas leptonychotis sp. nov., isolated from Weddell seals in Antarctica},
url = {https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.003753},
volume = {70},
year = {2020}
}
TY - JOUR
ID - 1644478
AU - Nováková, Dana - Švec, Pavel - Zeman, Michal - Busse, Hans-Jürgen - Mašlaňová, Ivana - Pantůček, Roman - Králová, Stanislava - Krištofová, Lucie - Sedláček, Ivo
PY - 2020
TI - Pseudomonas leptonychotis sp. nov., isolated from Weddell seals in Antarctica
JF - International Journal of systematic and Evolutionary Microbiology
VL - 70
IS - 1
SP - 302-308
EP - 302-308
PB - Microbiology Society
SN - 14665026
KW - Pseudomonas leptonychotis
KW - Antarctica
KW - Weddell seal
KW - polyphasic taxonomy
UR - https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.003753
L2 - https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.003753
N2 - A taxonomic study was carried out on four Gram-stain-negative strains P5773(T), P6169, P4708 and P6245, isolated from anus or mouth samples of Weddell seals at James Ross Island, Antarctica. The results of initial 16S rRNA gene sequence analysis showed that all four strains formed a group placed in the genus Pseudomonas and found Pseudomonas guineae and Pseudomonas peli to be their closest neighbours with 99.9 and 99.2% sequence similarity, respectively. Sequence analysis of rpoD, rpoB and gyrB housekeeping genes confirmed the highest similarity of isolates to P. peli (rpoD) and to P. guineae (rpoB and gyrB). The average nucleotide identity value below 86%, as calculated from the whole-genome sequence data, showed the low genomic relatedness of P5773(T) to its phylogenetic neighbours. The complete genome of strain P5773(T) was 4.4 Mb long and contained genes encoding proteins with biotechnological potential. The major fatty acids of the seal isolates were summed feature 8 (C-18:1 omega 7c), summed feature 3 (C-16 :1 omega 7c/C-16:1 omega 6c) and C-16:0. The major respiratory quinone was Q9. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. Putrescine and spermidine are predominant in the polyamine pattern. Further characterization performed using repetitive sequence-based PCR fingerprinting and MALDI-TOF MS analysis showed that the studied isolates formed a coherent cluster separated from the remaining Pseudomonas species and confirmed that they represent a novel species within the genus Pseudomonas, for which the name Pseudomonas leptonychotis sp. nov. is suggested. The type strain is P5773(T) (=CCM 8849(T)=LMG 30618(T)).
ER -
NOVÁKOVÁ, Dana, Pavel ŠVEC, Michal ZEMAN, Hans-Jürgen BUSSE, Ivana MAŠLAŇOVÁ, Roman PANTŮČEK, Stanislava KRÁLOVÁ, Lucie KRIŠTOFOVÁ a Ivo SEDLÁČEK. Pseudomonas leptonychotis sp. nov., isolated from Weddell seals in Antarctica. \textit{International Journal of systematic and Evolutionary Microbiology}. London: Microbiology Society, roč.~70, č.~1, s.~302-308. ISSN~1466-5026. doi:10.1099/ijsem.0.003753. 2020.
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