| BINÓ, Lucia, Iva VESELÁ, Iva PAPEŽÍKOVÁ, Jiřina PROCHÁZKOVÁ, Ondřej VAŠÍČEK, Kateřina ŠTEFKOVÁ, Jan KUČERA, Markéta HANÁČKOVÁ, Lukáš KUBALA and Jiří PACHERNÍK. The depletion of p38alpha kinase upregulates NADPH oxidase 2/NOX2/gp91 expression and the production of superoxide in mouse embryonic stem cells. Archives of Biochemistry and Biophysics. Elsevier Inc., 2019, vol. 671, August 2019, p. 18-26. ISSN 0003-9861. Available from: https://dx.doi.org/10.1016/j.abb.2019.06.001. |
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@article{1645656,
author = {Binó, Lucia and Veselá, Iva and Papežíková, Iva and Procházková, Jiřina and Vašíček, Ondřej and Štefková, Kateřina and Kučera, Jan and Hanáčková, Markéta and Kubala, Lukáš and Pacherník, Jiří},
article_number = {August 2019},
doi = {http://dx.doi.org/10.1016/j.abb.2019.06.001},
keywords = {Embryonic stem cell; p38 kinase; Reactive oxygen species; NADPH oxidase},
language = {eng},
issn = {0003-9861},
journal = {Archives of Biochemistry and Biophysics},
title = {The depletion of p38alpha kinase upregulates NADPH oxidase 2/NOX2/gp91 expression and the production of superoxide in mouse embryonic stem cells},
url = {https://www.sciencedirect.com/science/article/pii/S0003986118303813},
volume = {671},
year = {2019}
}
TY - JOUR
ID - 1645656
AU - Binó, Lucia - Veselá, Iva - Papežíková, Iva - Procházková, Jiřina - Vašíček, Ondřej - Štefková, Kateřina - Kučera, Jan - Hanáčková, Markéta - Kubala, Lukáš - Pacherník, Jiří
PY - 2019
TI - The depletion of p38alpha kinase upregulates NADPH oxidase 2/NOX2/gp91 expression and the production of superoxide in mouse embryonic stem cells
JF - Archives of Biochemistry and Biophysics
VL - 671
IS - August 2019
SP - 18-26
EP - 18-26
PB - Elsevier Inc.
SN - 00039861
KW - Embryonic stem cell
KW - p38 kinase
KW - Reactive oxygen species
KW - NADPH oxidase
UR - https://www.sciencedirect.com/science/article/pii/S0003986118303813
L2 - https://www.sciencedirect.com/science/article/pii/S0003986118303813
N2 - P38alpha kinase plays an important role in the regulation of both cell stress response and cell fate. In this study, we report that p38alpha kinase-deficient embryonic stem cells exhibit a higher production of reactive oxygen species (ROS) in contrast to their wild-type counterpart. Analysis of the expressions of NADPH oxidases (NOXs) and dual oxidases, crucial enzymes involved in intracellular ROS formation, shows NOX2/gp91phox is over-expressed in p38alpha deficient cells. The particular increase in superoxide formation was confirmed by the specific detection of hydroethidine derivate 2-hydroxyethidium. ROS formation decreased when the level of NOX2 was silenced by siRNA in p38alpha deficient cells. These data suggest the importance of p38alpha kinase in the regulation of ROS metabolism in embryonic stem cells and the significance of the observed phenomena of cancer cell-like phenotypes, which is discussed.
ER -
BINÓ, Lucia, Iva VESELÁ, Iva PAPEŽÍKOVÁ, Jiřina PROCHÁZKOVÁ, Ondřej VAŠÍČEK, Kateřina ŠTEFKOVÁ, Jan KUČERA, Markéta HANÁČKOVÁ, Lukáš KUBALA and Jiří PACHERNÍK. The depletion of p38alpha kinase upregulates NADPH oxidase 2/NOX2/gp91 expression and the production of superoxide in mouse embryonic stem cells. \textit{Archives of Biochemistry and Biophysics}. Elsevier Inc., 2019, vol.~671, August 2019, p.~18-26. ISSN~0003-9861. Available from: https://dx.doi.org/10.1016/j.abb.2019.06.001.
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