The depletion of p38alpha kinase upregulates NADPH oxidase
2/NOX2/gp91 expression and the production of superoxide in
mouse embryonic stem cells

J 2019

The depletion of p38alpha kinase upregulates NADPH oxidase 2/NOX2/gp91 expression and the production of superoxide in mouse embryonic stem cells

BINÓ, Lucia, Iva VESELÁ, Iva PAPEŽÍKOVÁ, Jiřina PROCHÁZKOVÁ, Ondřej VAŠÍČEK et. al.

Basic information

Original name

The depletion of p38alpha kinase upregulates NADPH oxidase 2/NOX2/gp91 expression and the production of superoxide in mouse embryonic stem cells

Authors

BINÓ, Lucia (703 Slovakia, belonging to the institution), Iva VESELÁ (203 Czech Republic, belonging to the institution), Iva PAPEŽÍKOVÁ, Jiřina PROCHÁZKOVÁ (203 Czech Republic), Ondřej VAŠÍČEK, Kateřina ŠTEFKOVÁ (203 Czech Republic, belonging to the institution), Jan KUČERA (203 Czech Republic, belonging to the institution), Markéta HANÁČKOVÁ (203 Czech Republic, belonging to the institution), Lukáš KUBALA (203 Czech Republic) and Jiří PACHERNÍK (203 Czech Republic, guarantor, belonging to the institution)

Edition

Archives of Biochemistry and Biophysics, Elsevier Inc. 2019, 0003-9861

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 3.391

RIV identification code

RIV/00216224:14310/19:00108263

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1016/j.abb.2019.06.001

UT WoS

000525438900003

Keywords in English

Embryonic stem cell; p38 kinase; Reactive oxygen species; NADPH oxidase

Abstract

V originále

P38alpha kinase plays an important role in the regulation of both cell stress response and cell fate. In this study, we report that p38alpha kinase-deficient embryonic stem cells exhibit a higher production of reactive oxygen species (ROS) in contrast to their wild-type counterpart. Analysis of the expressions of NADPH oxidases (NOXs) and dual oxidases, crucial enzymes involved in intracellular ROS formation, shows NOX2/gp91phox is over-expressed in p38alpha deficient cells. The particular increase in superoxide formation was confirmed by the specific detection of hydroethidine derivate 2-hydroxyethidium. ROS formation decreased when the level of NOX2 was silenced by siRNA in p38alpha deficient cells. These data suggest the importance of p38alpha kinase in the regulation of ROS metabolism in embryonic stem cells and the significance of the observed phenomena of cancer cell-like phenotypes, which is discussed.

Links

GA17-05466S, research and development project

Name: Role kanonické signální dráhy Wnt v neurogenezi. (Acronym: WntNeuro)

Investor: Czech Science Foundation

MUNI/A/1145/2017, interní kód MU

Name: Podpora výzkumné činnosti studentů v oblasti fyziologie, vývojové biologie a imunologie živočichů v roce 2018 (Acronym: OFIŽ 2018)

Investor: Masaryk University, Category A

Citovat

BINÓ, Lucia, Iva VESELÁ, Iva PAPEŽÍKOVÁ, Jiřina PROCHÁZKOVÁ, Ondřej VAŠÍČEK, Kateřina ŠTEFKOVÁ, Jan KUČERA, Markéta HANÁČKOVÁ, Lukáš KUBALA and Jiří PACHERNÍK. The depletion of p38alpha kinase upregulates NADPH oxidase 2/NOX2/gp91 expression and the production of superoxide in mouse embryonic stem cells. Archives of Biochemistry and Biophysics. Elsevier Inc., 2019, vol. 671, August 2019, p. 18-26. ISSN 0003-9861. Available from: https://dx.doi.org/10.1016/j.abb.2019.06.001.

@article{1645656,
   author = {Binó, Lucia and Veselá, Iva and Papežíková, Iva and Procházková, Jiřina and Vašíček, Ondřej and Štefková, Kateřina and Kučera, Jan and Hanáčková, Markéta and Kubala, Lukáš and Pacherník, Jiří},
   article_number = {August 2019},
   doi = {http://dx.doi.org/10.1016/j.abb.2019.06.001},
   keywords = {Embryonic stem cell; p38 kinase; Reactive oxygen species; NADPH oxidase},
   language = {eng},
   issn = {0003-9861},
   journal = {Archives of Biochemistry and Biophysics},
   title = {The depletion of p38alpha kinase upregulates NADPH oxidase 2/NOX2/gp91 expression and the production of superoxide in mouse embryonic stem cells},
   url = {https://www.sciencedirect.com/science/article/pii/S0003986118303813},
   volume = {671},
   year = {2019}
}

TY  - JOUR
ID  - 1645656
AU  - Binó, Lucia - Veselá, Iva - Papežíková, Iva - Procházková, Jiřina - Vašíček, Ondřej - Štefková, Kateřina - Kučera, Jan - Hanáčková, Markéta - Kubala, Lukáš - Pacherník, Jiří
PY  - 2019
TI  - The depletion of p38alpha kinase upregulates NADPH oxidase 2/NOX2/gp91 expression and the production of superoxide in mouse embryonic stem cells
JF  - Archives of Biochemistry and Biophysics
VL  - 671
IS  - August 2019
SP  - 18-26
EP  - 18-26
PB  - Elsevier Inc.
SN  - 00039861
KW  - Embryonic stem cell
KW  - p38 kinase
KW  - Reactive oxygen species
KW  - NADPH oxidase
UR  - https://www.sciencedirect.com/science/article/pii/S0003986118303813
L2  - https://www.sciencedirect.com/science/article/pii/S0003986118303813
N2  - P38alpha kinase plays an important role in the regulation of both cell stress response and cell fate. In this study, we report that p38alpha kinase-deficient embryonic stem cells exhibit a higher production of reactive oxygen species (ROS) in contrast to their wild-type counterpart. Analysis of the expressions of NADPH oxidases (NOXs) and dual oxidases, crucial enzymes involved in intracellular ROS formation, shows NOX2/gp91phox is over-expressed in p38alpha deficient cells. The particular increase in superoxide formation was confirmed by the specific detection of hydroethidine derivate 2-hydroxyethidium. ROS formation decreased when the level of NOX2 was silenced by siRNA in p38alpha deficient cells. These data suggest the importance of p38alpha kinase in the regulation of ROS metabolism in embryonic stem cells and the significance of the observed phenomena of cancer cell-like phenotypes, which is discussed.
ER  -

BINÓ, Lucia, Iva VESELÁ, Iva PAPEŽÍKOVÁ, Jiřina PROCHÁZKOVÁ, Ondřej VAŠÍČEK, Kateřina ŠTEFKOVÁ, Jan KUČERA, Markéta HANÁČKOVÁ, Lukáš KUBALA and Jiří PACHERNÍK. The depletion of p38alpha kinase upregulates NADPH oxidase 2/NOX2/gp91 expression and the production of superoxide in mouse embryonic stem cells. \textit{Archives of Biochemistry and Biophysics}. Elsevier Inc., 2019, vol.~671, August 2019, p.~18-26. ISSN~0003-9861. Available from: https://dx.doi.org/10.1016/j.abb.2019.06.001.

Detailed Information on Publication Record