Detailed Information on Publication Record
2020
Administration of the Probiotic Escherichia coli Strain A0 34/86 Resulted in a Stable Colonization of the Human Intestine During the First Year of Life
MICENKOVÁ, Lenka, Juraj BOSÁK, Stanislav SMATANA, Adam NOVOTNÝ, Eva BUDINSKÁ et. al.Basic information
Original name
Administration of the Probiotic Escherichia coli Strain A0 34/86 Resulted in a Stable Colonization of the Human Intestine During the First Year of Life
Authors
MICENKOVÁ, Lenka (703 Slovakia, belonging to the institution), Juraj BOSÁK (703 Slovakia, belonging to the institution), Stanislav SMATANA (703 Slovakia, belonging to the institution), Adam NOVOTNÝ (203 Czech Republic, belonging to the institution), Eva BUDINSKÁ (703 Slovakia, belonging to the institution) and David ŠMAJS (203 Czech Republic, guarantor, belonging to the institution)
Edition
Probiotics and Antimicrobial Proteins, New York, Springer New York LLC, 2020, 1867-1306
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10606 Microbiology
Country of publisher
United States of America
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 4.609
RIV identification code
RIV/00216224:14110/20:00114097
Organization unit
Faculty of Medicine
UT WoS
000541342000003
Keywords in English
Colinfant; E. coli; Enterobacteriaceae; Probiotic; Sequencing
Tags
International impact, Reviewed
Změněno: 17/8/2020 10:26, Mgr. Tereza Miškechová
Abstract
V originále
Colinfant New Born (CNB) is an orally administered probiotic preparation containing theEscherichia colistrain A0 34/86, which is specially marketed for use in newborns and infants. Although the impact of different probiotics on the composition of the human gut microbiota has been previously described, the effects ofE. coliprobiotic consumption during infancy on the development of intestinal microbiota are not known. The effect of oral administration of CNB on theEnterobacteriaceaepopulation was mapped using 16S rRNA gene sequencing in DNA samples isolated from the stools of one infant collected at 177 different time points during the first year of life.E. colistrains turnover was analyzed based on the detection of 26 genetic determinants, phylogroups, and pulsed-field gel electrophoresis (PFGE) analysis. Administration of CNB during the second and third month of life introduced theEscherichiagenus to the infant's intestinal tract, andEscherichiabecame dominant among theEnterobacteriaceaefamily (p < 0.01). Genetic determinants, typical for probioticE. coliA0 34/86 strain, were detected on the first day after application of CNB and persisted all year. In addition, nine transientE. colistrains were identified; these strains harbored different genetic determinants and showed different PFGE profiles. Transient strains were detected from 2 to 24 days in the stool samples. The firstEscherichiacolonizer originated from the application of the CNB probiotic preparation. ProbioticE. coliA0 34/86 successfully colonized the intestinal tract of an infant and became resident during the first year of life.
Links
| EF15_003/0000469, research and development project |
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| EF16_013/0001761, research and development project |
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| GA16-21649S, research and development project |
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| GJ17-24592Y, research and development project |
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| LM2015051, research and development project |
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| LM2015085, research and development project |
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| MUNI/M/1322/2015, interní kód MU |
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